iPS Cells (iPSC from Fibroblasts, Episomal, Male, Control Lines for Genome Editing Services) *Academic Price

SKU :
ASE-9203
Catalog # :
ASE-9203
Size :
2 -5x 10^5 cells/vial
$1,260.00
Overview

Applied StemCell, Inc. provides Induced Pluripotent Stem (iPS) cells with low passages (p12). These pluripotent cells were generated from normal human skin fibroblast using a non-integrating, footprint-free reprogramming method (1).  This method allows the transient expression of human transcription factors (OCT4, SOX2, KLF4, and c-MYC) that initiate the reprogramming process (2). The resulting human iPS cells (hiPSCs) were selected using morphological criteria without the use of either fluorescent markers or drug selection. These iPS cells have been tested for the expression of the pluripotency markers, including Oct4, Sox2, SSEA4, and TRA-1-60, AP (alkaline phosphatase) activity and for functional pluripotency via teratoma formation analysis. Detailed protocols for thawing, culturing under both feeder and feeder-free conditions, and cryopreserving of these iPS cells are provided. 

Teratoma Formation Analysis

Histological analyses using H&E staining of kidney and testis tumors from mice injected with the ASE-9203 iPSC line. Differentiated tissues representing the three germ layers are shown and indicated by arrow heads. Abbreviations: EN: endoderm; ME: mesoderm; EC: ectoderm. 

teratoma-3teratoma-1 teratoma-2 

 EN: Gland (200x)                                  ME: Cartilage (200x)                             EC: Neuronal Rosette (200x)

Karyotype Analysis

karyotype

Karyotype analysis to rule out genetic aberrations. Cytogenic analysis was performed on G-banded metaphase cells from human iPSC line, ASE-9203. This iPSC line demonstrates a normal male karyotype.

Pluripotency Marker Analysis

marker

Expression of pluripotency markers. ASC 9203 iPS cell line expresses common iPSC biomarkers (OCT-4, TRA-1-60, TRA-1-81 SSEA-4 and SOX-2).  The corresponding DAPI staining is below each image.  All images were taken at 10x magnification.

Alkaline Phosphatase (AP) staining

AP

 ASC-9203 iPSCs stain positive for Alkaline Phosphatase: (a) A typical unstained colony (a) was used to gauge the extent of the AP staining (b). Both images were taken at 5x magnification.