TARGATT™ CHO Master Cell Line and Knock-in Kit

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1 Kit

The TARGATT™ CHO-S Master Cell Line and transgenic kit was designed for fast and site-specific knock-in in Chinese Hamster Ovary (CHO) cells using an easy-to-use gene knock-in approach. The master cell line provided in this kit contains both the “attP” docking-site and the PhiC31 integrase expression cassette engineered into the CHO H11 safe harbor locus in the genome. Any gene of interest can be cloned into the provided TARGATT™ “attB” cloning plasmid (under control of the strong CAG promoter or promoter-of-choice), and transfected into the master cell line for generating a stable knock-in cell line. The TARGATT™ integrase-based technology enables efficient DNA integration and high-level gene expression without disrupting internal genes. The TARGATT™ CHO-S cell line can therefore be used for uniform, site-specific gene knock-in, generation of isogenic cell lines, and amplification strategies for isolating high expression cells, without the need for single cell cloning.

The TARGATT™ CHO-S master cell line and transgenic kit are suitable for research applications involving gene overexpression and high-level expression of recombinant proteins and other biologics in a rapidly expanding bioproduction industry and for other applications*.

 Advantages of using TARGATT™ Master Cell Lines for gene knock-in:

  • Stable cell line generation
  • Single copy, site-specific gene integration into the safe harbor locus
  • High-level gene expression from an active, intergenic locus
  • Use of selection marker is not necessary but optional
  • Easy-to-use protocol requiring transfection of only one (donor) plasmid

*TARGATT™ master cell lines can be generated in any cell line including stem cells. Please contact Applied StemCell for TARGATT™ cell line engineering services to generate a master cell line in a specific cell line of choice.

TARGATT™ Master Cell Line Publication:

  • Chi, X., Zheng, Q., Jiang, R., Chen-Tsai, R. Y., & Kong, L. J. (2019). A system for site-specific integration of transgenes in mammalian cells. PLOS ONE14(7), e0219842.