Newsletter
CHO Cells
TARGATT™ for Bioproduction of Recombinant Proteins in Chinese Hamster Ovary (CHO) cells.
The traditional CHO antibody production method is very inefficient because it involves random gene insertion and forced amplification of transgenes. Many factors adversely affect bioproduction levels. These factors include insertion sites effects (position effects), DNA repeat-induced gene silencing and genomic instability, altered regulation or interruption of endogenous genes.
Thus, the goals of the CHO antibody production field are: a defined genomic locus with guaranteed high levels of expression, high integration efficiency and easy screening that is reproducible for any candidate protein. In addition, it is crucial that the cell line is viable and stably reproduced.
Applied StemCell has met and exceeded these goals with its proprietary TARGATT™ technology in CHO cell lines. Applied StemCell (ASC) is proud to announce the launch of our CHO Bioproduction cell line technology. ASC has created Master TARGATT™ CHO cell lines for the rapid creation of new lines for high level protein and antibody expression. Applied StemCell’s TARGATT™ CHO master cell lines yield >2.5g/L of recombinant proteins in a 2-week fed-batch shake flask expression system and is easily scalable for large scale bioproduction.
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Features |
TARGATT™ Site-Specific Insertion |
Traditional Random Gene Insertion |
|
Safe Genomic Locus |
Yes |
No; random |
|
Protein Yield |
High |
Varies |
|
Gene Insertion Efficiency |
High |
Low |
|
Reproducibility |
Yes; for any protein |
No; different from one protein to another |
|
Copy # of Inserted Gene |
Single copy |
Multiple copies |
|
Stable |
Yes |
No; Chromosome rearrangement |
|
Gene Silencing |
No |
Yes |
Our TARGATT™ technology improves CHO antibody production capability that, in turn, reduces the cost of bioproduction and manufacturing. Our technology makes CHO antibody production feasible and economical for companies and projects of all sizes. Please let us know if you have questions about your CHO bioproduction project
.
Technical Details
Technical Details for Bioproduction in TARGATT™ CHO Cells
Antibody Production in TARGATT™ CHO-H11 cells
Figure 1. Expression of antibodies Ab1 and Ab2 in the H11 genomic hotspot locus of the TARGATT™ CHO-H11 cells yielded ~1g/L of each protein.
Optimizing Docking Site Location in TARGATT™ CHO Cell Lines
Figure 2. Expression of green fluorescent protein (GFP) in various genomic hotspot loci in TARGATT™ CHO cells. TARGATT™ CHO master cell lines were engineered with attP docking site in the well-characterized H11 locus and 4 different proprietary loci, ASC1, ASC2, ASC3, and ASC4. The GFP reporter gene was inserted into each cell line and its expression from each locus was measured using FACS analysis. Reporter expression in the ASC2 locus was 2.5-fold higher than the expression in the H11 locus. The estimated protein yield from the TARGATT™ CHO-ASC2 Master cell line is ~2.5 g/L.
Ordering
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