• CHO Cells

CHO Cells

TARGATT™ Chinese Hamster Ovary (CHO) Cells for Recombinant Proteins and Antibody Bioproduction

Applied StemCell has created Master TARGATT™ CHO cell lines for the rapid creation of new lines for high level protein and antibody expression. The TARGATT™ CHO master cell lines yield >2.5g/L of recombinant proteins in a 2-week fed-batch shake flask expression system and is easily scalable for large scale bioproduction. 

Our Master TARGATT™ CHO cell lines exceed the capabilities of the traditionally made CHO antibody production cells and also reduces the cost of bioproduction and manufacturing. Our technology makes CHO antibody production feasible and economical for companies and projects of all sizes.

Please let us know how we can help you with your CHO bioproduction project!

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Application Notes

Bioproduction in TARGATT™ CHO Cells

Antibody Production in TARGATT™ CHO-H11 Cells

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Figure 1. Expression of antibodies Ab1 and Ab2 in the H11 genomic hotspot locus of the TARGATT™ CHO-H11 cells yielded ~1g/L of each protein.

Optimizing Docking Site Location in TARGATT™ CHO Cell Lines

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Figure 2. Expression of green fluorescent protein (GFP) in various genomic hotspot loci in TARGATT™ CHO cells. TARGATT™ CHO master cell lines were engineered with attP docking site in the well-characterized H11 locus and 4 different proprietary loci, ASC1, ASC2, ASC3, and ASC4. The GFP reporter gene was inserted into each cell line and its expression from each locus was measured using FACS analysis. Reporter expression in the ASC2 locus was 2.5-fold higher than the expression in the H11 locus. The estimated protein yield from the TARGATT™ CHO-ASC2 Master cell line is ~2.5 g/L.

Technical Details

The traditional CHO antibody production method is very inefficient because it involves random gene insertion and forced amplification of transgenes. Many factors adversely affect bioproduction levels. These factors include insertion sites effects (position effects), DNA repeat-induced gene silencing and genomic instability, altered regulation or interruption of endogenous genes.

Thus, the goals of CHO antibody production are: a defined genomic locus with guaranteed high levels of expression, high integration efficiency and easy screening that is reproducible for any candidate protein. In addition, it is crucial that the cell line is viable and stably reproduced.

Applied StemCell has met and exceeded these goals with its proprietary TARGATT™ technology in CHO cell lines.

Features

TARGATT™ Site-Specific Insertion

Traditional Random Gene Insertion

Safe Genomic Locus

Yes

No; random

Protein Yield

High

Varies

Gene Insertion Efficiency

High

Low

Reproducibility

Yes; for any protein

No; different from one protein to another

Copy # of Inserted Gene

Single copy

Multiple copies

Stable

Yes

No; Chromosome rearrangement

Gene Silencing

No

Yes

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!