Newsletter
Rabbit Monoclonal Antibody Development
Applied StemCell (ASC) is a fast-growing gene and cell therapy CRO that continuously works to expand its technologies, services, and products to address the current and future needs of the industry and research community. ASC understands that monoclonal antibodies (mAbs) are essential for research and development, so we established and optimized a single B cell antibody discovery platform that can be used to generate high-quality rabbit or alpaca antibodies. Choose from our selection of unique rabbit strains/lines, or ask us about our customized gene knockout rabbit. You can select our classical or accelerated immunization protocol based on your timeline constraints. Once the immunization process is complete, our team conducts single B cell cloning and delivers 40 ELISA positive recombinant mAbs and a data report. You can screen and select your clones of interest, or ASC can complete the screening process for you (*additional fees apply). After antibody selection, our scientists sequence and produce the elected antibodies.
During your free consultation, our experts can help you design a flexible project outline that captures all of your requirements. Once the project design is finalized, you send us your antigen and we take care of the rest. ASC will have your rabbit monoclonal antibodies in just a few weeks.
- Rabbit Monoclonal Antibodies with Subnanomolar Affinity
- HyImmune Rabbits typically produce 3× antigen specific B cells, to increase the antibody development rate
- Unlimited Supply: Customer owns IP
- More Antibodies: >0.5mg for each antibody selected
- Convenient: Antibodies can be delivered with or without luciferase
- Alpaca Antibody Service Available - Inquire
- Affordable Pricing
- Fast turnaround time
Advantages of ASC's Single B Cell Antibody Discovery Platform
Optimized Immunization Protocol
Rapid Amplification of Single B Cell mRNA
Efficient Mammalian Cell Antibody Expression
Rabbit Antibody Production Better than Hybridoma and Phase Display
Why Rabbit & Alpaca?
Rabbit mAbs | Alpaca mAbs |
|
|
Service Overview
Stage 1: Customer Provides Antigen |
Stage 2: Animal Immunization |
Stage 3: Single B Cell Cloning |
Stage 4: Customer In House Screening |
Stage 5: Antibody Sequencing & Production |
|
|
|
Additional Service: Assay Development |
|
Supplemental Information
- Serum Collection
- Prebleed
- Postbleed 1 – After 3 Immunization Injections
- Postbleed 2 – After 4 Immunization Injections
Additional Services
Stage 0: Antigen Preparation |
Stage 6: Cell Line Generation & Assay Development |
|
|
Deliverables
Stage 2 | Stage 3 | Stage 5 |
|
|
|
Sample Reports:
Products and Services
Case Studies
Case Study 1: Identification of Antibodies for COVID-19 Antigen Detection
Rabbit Line: LucAb Rabbit
Reasons For Using the Rabbit Line: Luciferase allows quantification of antibodies and enables sandwich ELISA setup; Sandwich ELISA = used to detect ultrasensitive antibody pairs
Results: The antibody pair that we identified can be incorporated into a sandwich ELISA, and it can detect 0.5 pg/ml COVID-19 Nucleocapsid in human serum samples.
*Sensitivity is much higher than other products
Figure 1: COVID-19 Antigen Detection by the ASC ELISA Kit. Low levels of COVID-19 antigen were detected in human serum samples with the ASC ELISA kit.
Vendors | Detection Limit |
Applied StemCell |
0.5 pg/ml |
Competitor A |
70 pg/ml |
Competitor B |
~1000 pg/ml |
Competitor C |
70 pg/ml |
Competitor D | 46.68 pg/ml |
Competitor E |
120 pg/ml |
Competitor F | 3.2 pg/ml |
Competitor G |
780 pg/ml |
Competitor H |
21.4 pg/ml |
*Table 1: Low Detection Limits of Other Vendor ELISA kits.
Case Study 2: Detection of VEGFA Antibodies for Therapeutics
Rabbit Line: Hu Rabbit
Reasons For Using the Rabbit Line: The antibodies produced by the Hu rabbit are more like human antibodies because they do not contain the disulfide bond found in most regular antibodies.
*Antibodies can be humanized successfully
Results: 11 bioactive mAbs detected; The 11 mAbs can be engineered into chimeric antibodies that hold human constant regions while maintaining high affinities (10x higher than Avastin)
Figure 2: Antibody Affinity. Bioactive, chimeric antibodies display higher affinities than Competitor I.
# mAbs expressed: 150 |
# high-affinity mAbs: 43 |
# Bioactive mAbs: 11 |
# Bioactive chimeric: 11 |
Table 2: Breakdown of Antibodies Expressed. Out of the 150 expressed mAbs, 43 were high-affinity antibodies. 11 bioactive antibodies were identified which can be engineered into chimeric antibodies.
Case Study 3: Identification of Fentanyl Antibodies For Drug Detection
Rabbit Line: HyImmune Rabbit
Reasons For Using the Rabbit Line: Rapid production of small target antibodies
Results: Several fentanyl antibodies were discovered.
*These antibodies were used to develop the ELISA kits. The ASC ELISA kit standard I-50 measurement was 10x more sensitive than other ELISA kits. The kit was even able to detect fentanyl derivatives.
Figure 2: ASC ELISA Kit I-50 Measurements.
How Our ELISA Kit Compares to Others |
ASC ELISA kit
|
Competitor J ELISA kit
|
Table 3: How the ASC ELISA kit I-50 Measurements Compare to Other Kits. The ASC ELISA kit is 10x more sensitive.
Case Study 4: Customized Knockout Rabbit Antibody Generation
Rabbit Line: Gene Knockout Rabbit
Reasons For Using the Rabbit Line: Knockout of the endogenous homolog protein generates an "all epitope targeting" environment for antibody production. This increases the rate of bioactive antibody identification.
* We can generate multispecific antibodies for pre-clinical animal experiments
Results: Antibodies with high bioactivity were identified. These antibodies cross-react with both human and mouse targets.
Support Materials
FAQs
Which cell surface makers did you use to sort out rabbit B cells?
Could we used SBEAD approach to generate mAb against peptide having 8 to 12 amino acids long?
Would it be possible to use your SBEAD technology to generate the 40 positive ELISA clones and continue with antibody production in our lab or do you require production of the antibody in your facility only?
What is the cost of the Ab discovery via HyImmune rabbit and SBEAD protocol?
Is the Hyimmune rabbit lacking the LC C-C bond wildtype or engineered rabbit?
What is the timeline from immunization to clone sequence delivery?
How many Ab clones are delivered for each immunization.
Does ASC do antibody humanization?
Could the client obtain the leftover B cells from the SBEAD screening and make a phage display library from it?
Can you also generate polyclonal antibodies in parallel with monoclonal antibody development?
Can SBEAD be applied to human samples?
Can antibodies be developed with low amount of antigen, e.g. <0.5mg?
Our unique amino acid sequence of our protein is only 8 aa long. If this sequence is too short, we could extend it N-terminally. We prefer a rabbit antibody, is this possible?
Do you also offer polyclonal antibody development?
Have you generated mAB against small molecules (160 g/mol)?
Are you also able to provide the hybridoma clones?
Plant Antibodies: If we provide a particular nucleotide sequence of a plant's gene, would you be able to provide an antibody targeting the protein that is expressed in the plant?
Zebrafish Antibodies: Can you provide the zebrafish monoclonal antibodies? I am interested in developing anti-zebrafish antibodies (this would be for zebrafish proteins). What information do I need to submit?