• Transgenic Animals - Rabbit

Transgenic Animals - Rabbit

TARGATT™ Rabbit for Biopharming and High-Yield Recombinant Protein and Antibody Production

Animal bioproduction uses transgenic animal mammary gland as a bioreactor for the production of recombinant proteins. Animal bioproduction, compared to CHO bioproduction, has the advantages of lower upfront and maintenance costs; is easy to contain, control and transport; involves faster development processes and no scale-up issues; and has a unique low-cost bulk holding stage (frozen milk). However existing technological methods such as random integration to create transgenic animals have many limitations. Applied StemCell has overcome these limitations with our proprietary TARGATT™ technology to reproducibly, and consistently, control the knock-in locus, copy number and expression of the target protein in transgenic animals.

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Application Notes

Bioproduction Using TARGATT™ Rabbits

Expression of TdTomato fluorescent protein in rabbit milk

TECHNICAL-bioproduction-rabbit-1

Figure 1. TdTomato expression in TARGATT™ Bioproduction Rabbit. TARGATT™ rabbit was engineered to express the 56 kDa tdTomato protein from the endogenous beta-casein locus. The yield of tdTomato protein was > 5g/L from a heterozygous rabbit (red color is due to TdTomato expression) as compared to 26 kDa rabbit beta-casein protein from a wild type rabbit.

Technical Details

Advantages of Applied StemCell’s TARGATT™ technology over existing methods to create transgenic animals.

Existing Challenges

TARGATT™ Solution

  • Random insertion, no control of insertion locus or copy number
  •  Control of locus and copy number using targeted knock-in
  •  Low yield of recombinant protein: 1-2 g/L for old method
  •  High yield of recombinant protein by using highly expressing locus b-casein
  •  Inconsistent from one protein to another
  •  Consistent protein production using a high efficiency and highly reproducible knock-in strategy

Applied StemCell’s TARGATT™ technology utilizes the PhiC31 integrase and attP docking sites to direct the chosen target protein to a pre-selected and well characterized locus, with high efficiency. Our method ensures high expression and eliminates position effects such as gene silencing, genomic instability and interruption of endogenous genes.

Advantages of Animal Bioproduction (Biopharming):

  • Very low upfront investment and low maintenance costs (~10 times lower than CHO)
  • Easy to contain, control and transport
  • Speed of development and expansion
  • No up-scaling problems, size of individual bioreactor constant
  • Technically less demanding
  • Allows complex protein production
  • Unique early low-cost bulk holding stage (frozen milk)
  • Easy protein isolation and purification

We are excited to use our TARGATT™technology to rapidly engineer your bioproduction animal with your gene of interest. Please contact us for details, pricing and timelines for your specific project.

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!