Transgenic Animals - Rabbit

TARGATT™ Genome Engineering for Animal Bioproduction

Animal bioproduction uses transgenic animal mammary gland as a bioreactor for the production of recombinant proteins. Animal bioproduction, compared to CHO bioproduction, has the advantages of lower upfront and maintenance costs, is easy to contain, control and transport, involves faster development processes and no scale-up issues, and has a unique low-cost bulk holding stage (frozen milk). Existing methods used to create transgenic animals for the bioproduction of important recombinant proteins, has many limitations. For example, target genes are randomly inserted, there is no control of copy number and the yield of recombinant protein is low, usually between 1-2g/L. In addition, each new protein is a new project with different and unpredictable outcomes.

Existing Challenges

TARGATT™ Solution

  • Random insertion, no control of insertion locus or copy number
  •  Control of locus and copy number using targeted knock-in
  •  Low yield of recombinant protein: 1-2 g/L for old method
  •  High yield of recombinant protein by using highly expressing locus b-casein
  •  Inconsistent from one protein to another
  •  Consistent protein production using a high efficiency and highly reproducible knock-in strategy

Applied StemCell has pioneered a new method to generate transgenic animals using our proprietary TARGATT™ technology. This technology allows us to reproducibly, and consistently, control the knock-in locus, copy number and expression of the target protein. TARGATT™ utilizes the PhiC31integrase and attP docking sites to direct the chosen target protein, with high efficiency, to a pre-selected and well characterized locus. Our method ensures high expression and eliminates position effects such as gene silencing, genomic instability and interruption of endogenous genes.

We are excited to use our TARGATTtechnology to rapidly engineer your bioproduction animal with your gene of interest. Please contact us for details, pricing and timelines for your specific project. 

Advantages of Animal Bioproduction (Biopharming):

  • Very low upfront investment and low maintenance costs (~10 times lower than CHO)
  • Easy to contain, control and transport
  • Speed of development and expansion
  • No up-scaling problems, size of individual bioreactor constant
  • Technically less demanding
  • Allows complex protein production
  • Unique early low-cost bulk holding stage (frozen milk)
  • Easy protein isolating and purification
Technical Details

Technical Details for Bioproduction Using TARGATT™ Rabbits

Expression of TdTomato fluorescent protein in rabbit milk


Figure 1. TdTomato expression in TARGATT™ Bioproduction Rabbit. TARGATT™ rabbit was engineered to express the 56 kDa tdTomato protein from the endogenous beta-casein locus. The yield of tdTomato protein was > 5g/L from a heterozygous rabbit (red color is due to TdTomato expression) as compared to 26 kDa rabbit beta-casein protein from a wild type rabbit.

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