CRISPR Knock-in Cell Line Service (Human Primary, T Cells, & Blood Lineage Cell Lines)

SKU :
ASC-6012-4C
Catalog # :
ASC-6012-4C
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Overview

We offer efficient CRISPR-based stable knock-in cell line generation for hard-to-transfect cell lines such as blood lineage cells, primary T cells and fibroblasts, and more, using multi-approach designs and highly optimized CRISPR knock-in cell line protocols. ASC has generated 1300+ unique cell line models with a wide variety of modifications in 200+ mammalian cell lines, and we are one of the very few CRISPR cell line generation in hard-to-edit cell lines service providers who knows how to work with these difficult cell lines.

Other features include:

  • Homozygous/ heterozygous knock-in clones
  • Variety of cell lines: cancer, hard-to-transfect, most mammalian species

Workflow includes:

  • Cell line evaluation (culture conditions, single cell clonability, etc.)
  • gRNA design, construction, and validation
  • Donor DNA construction
  • Cell line validation, transfection and optimization
  • Screening for single cell clones and clone confirmation
  • Cell expansion and cryopreservation

Deliverables:

  • Two (2) vials of 2 x 10^5 cells/ vial of cell line with targeted insertion either as stable single cell clones or clonal pools (as determined by milestone 1).
  • Milestone update/report; final report with detailed description of each procedure, including targeting vector design, construction and validation, transfection condition, genotyping strategy, and results.

Timeline: 3-4 months

Applications:

  • Disease modeling for immuno-oncology, pharmacogenomic studies
  • Drug discovery and drug efficacy and toxicity screening; drug combination studies
  • Antibody validation/ screening
  • Deriving diagnostic reference standards and materials
  • Recombinant protein production in CHO cells
  • Generation of TARGATT™ master cell lines by inserting an attP "docking site" for site-specific gene knock-in