Vector Design / Cloning Service

Applied StemCell has decades of expertise in anything DNA-related. We can help you develop the best strategy to tackle your cloning projects, and find solutions to all your technical problems. 

  • Comprehensive services for genome engineering for in vivo and in vitro applications
  • Restriction fragment cloning
  • Design and validation of CRISPR/Cas9 components: gRNA design and in vitro validation by next generation sequencing (NGS)
  • Gene targeting vectors for homologous recombination
  • Design and construction of vectors for transient mammalian transgene expression
  • Bacterial artificial chromosome (BAC) recombineering for large fragment insertion
  • Design of RNAi and inducible vectors, gene tagging, and site-directed mutagenesis
  • Vector design for generation of random transgenic cell line and animal models

A typical cloning procedure includes:

  • Primer design for target region
  • PCR amplification of target region
  • Cloning of the PCR product into a cloning vector
  • Sub-cloning the gene into the vector of your choice
  • Picking, plating, culturing, and DNA preparation
  • Sequence confirmation
  • Plasmid purification (Mini, Midi, or Maxi size)
References

Applied StemCell's citations and publications in journals:

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