• Vector Design / Cloning Service

Vector Design / Cloning Service

Applied StemCell has extensive expertise in anything DNA-related. We can develop the best strategy to tackle your cloning projects, and find solutions to all your technical problems.

  • Comprehensive services for genome engineering for in vivo and in vitro applications
  • Restriction fragment cloning
  • Design and validation of CRISPR/Cas9 components: gRNA design and in vitro validation by next generation sequencing (NGS)
  • Gene targeting vectors for homologous recombination
  • Design and construction of vectors for transient mammalian transgene expression
  • Bacterial artificial chromosome recombineering for large fragment insertion
  • Design of RNAi and inducible vectors, gene tagging, and site-directed mutagenesis
  • Vector design for generation of random transgenic cell line and animal models
Products and Services

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Support Materials
Technical Details

Simplify your research workflow! Let Applied StemCell clone your gene of interest while you focus on other aspects of your project.

A typical cloning procedure includes:

  • Primer design for target region
  • PCR amplification of target region
  • Cloning of the PCR product into a cloning vector
  • Sub-cloning the gene into the vector of your choice
  • Picking, plating, culturing, and DNA preparation
  • Sequence confirmation
  • Plasmid purification (Mini, Midi, or Maxi size)
Publications

Applied StemCell's citations and publications in journals:

  • Hu, Q., Li, C., Wang, S., Li, Y., Wen, B., Zhang, Y., ... & Nguyen, T. K. (2019). LncRNAs-directed PTEN enzymatic switch governs epithelial–mesenchymal transition. Cell research, 1
  • Besschetnova, T. Y., Ichimura, T., Katebi, N., Croix, B. S., Bonventre, J. V., & Olsen, B. R. (2015). Regulatory mechanisms of anthrax toxin receptor 1-dependent vascular and connective tissue homeostasis. Matrix Biology, 42, 56-73
Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!