iPS Cells GMP Grade (iPSCs from CD34+ Cord Blood, Male)

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SKU :
ASE-9280
Catalog # :
ASE-9280
Format :
Frozen
Size :
1 x 10^6 cells/vial
  • Description
  • Related Products

ASC’s cGMP Grade iPSC Line

Applied StemCell’s cGMP Grade iPSC Line was generated from male, CD34+ cord blood using an episomal reprogramming method. Master cell bank or working cell bank are available.

Applied StemCell’s cGMP Grade iPSC Line

Source cell type: CD34+ Cord Blood, male

Reprogramming method: Episomal

Master cell bank or working cell bank available

Product QC:

  • Sterility/mycoplasma
  • Identity: SNP Trace
  • Pluripotency: image, AP stain, Oct4/TRA-1-60 stain, differentiation in vitro to 3 germ layers
  • Genetic stability: karyotyping by G-banding
  • Viability

iPSC Expansion and Banking

ASC provides iPSC expansion and banking of its cGMP iPSCs. We also provide custom iPSC generation services using an epsiomal reprogramming method.

cGMP iPSC Expansion and Banking

 

Number of Vials

Cell Count

Other Deliverables

Master Cell Bank

50-200 vials

1x10^6 cells/vial

QC and CoA

Working Cell Bank

50-200 vials

1x10^6 cells/vial

QC and CoA

 

Customized iPSC Generation (Episomal Method)

 

Number of Clones

Number of Vials

Cell Count

Other Deliverables

Seed Bank

3 Clones

10 vials

1x10^6 cells/vial

QC Characterization

and CoA

iPSC Characterization: Direct Differentiation to Three Germ Layers

Figure 1:  Direct differentiation of ASE-9280 GMP Grade iPSCs to three germ layers. Staining for lineage-specific biomarkers of three germ layers after direct differentiation of the GMP hiPSC line, ASE-9280. The ASE-9280 GMP hiPSC line was differentiated to specific lineages of the germ layers using well-established and optimized protocols. Immunostaining for biomarkers of each lineage was performed. Cells were also co-stained with nuclear marker, DAPI (blue). Biomarkers: Ectoderm (EC) marker: Pax6 (green) and Tuji (red); Mesoderm (ME) marker: Brachyury (red) and GATA-4 (green); Endoderm (EN) marker: Fox2A (red) and Sox-17 (green).

Visit our cGMP page for more details about our cGMP services ->

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