Astrocytes Differentiation from iPSC

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Astrocytes, the most abundant glial cells found in the central nervous system and spinal cord, play a critical role in supporting neuronal function and homeostasis as well as repair and neurogenesis during CNS and spinal cord injury. Astrocyte dysfunction has been implicated in neurological disorders such as Parkinson’s disease, Alzheimer’s disease, Amyotrophic lateral sclerosis (ALS), and Huntington’s disease. The iPSC-derived astrocytes provide a readily sourced, consistent, and biologically relevant alternative to primary astrocytes for the study of synaptic transmission and plasticity in normal CNS function and disease progression.

  • Delivered as robust, mature cells with morphology of human primary astrocytes
  • iPSC derived using different reprogramming strategies from fibroblast/ PBMC/ CD34+ cord blood cells
  • From healthy/ disease/ engineered iPSCs
  • Well-characterized “Master” control, human iPSC lines available for a ready start to your differentiation projects as well as control lines for your disease modeling experiments
  • High purity (>90%) GFAP+, and S100beta+ astrocyte-specific markers

Optional! RT-PCR, Western Blot, proliferation assays, calcium activity assays, cytokine-mediated inflammatory response assays 

  • Applications:
    • Co-culture with neurons for advanced, in vivo-like cell line models
    • Neurotoxicity screening
    • Disease modeling
    • High throughput drug discovery and drug screening applications
    • Drug neurotoxicity screening applications
  • Timeline = 3-5 months
  • Deliverables
    • Cryopreserved iPSC-derived astrocytes: >90% GFAP+; S100beta+
    • Antibody staining images and/or flow cytometry data for the cell type specific markers
    • Recovery Test, Mycoplasma Test
    • Sub-culture protocol and media (optional)
    • Other markers (available upon request)