CRISPR Custom Knock-in iPSC Generation Service

SKU :
ASC-6013-4
Catalog # :
ASC-6013-4
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Overview

Generate stable gene knock-in iPSC lines using our acclaimed and unique expertise in CRISPR iPSC/ ES Cell Genome Editing Service:

Also available, downstream iPSC differentiation and characterization services.

  • Optimized CRISPR protocols for high success rate
  • Footprint-free, feeder-free transfection and iPSC culture protocols
  • Isogenic control lines for reliable experiments
  • Knock-in a variety of genes for different applications
    • Locus-specific small/ large fragment gene insertions
    • Safe harbor locus knock-in
    • Reporter gene knock-in/ gene tagging
    • Gene overexpression (Ex. Antibody/ antigen expression)
    • Promoter modifications
    • Gene replacement; gene therapy
    • Gene fusion/ translocation

Workflow includes:

  • Cell line evaluation
  • gRNA design, CRISPR vector and donor DNA construction, and reagent validation
  • Transfection of targeting vectors and optimization
  • Screening for single cell clones and clone confirmation
  • Cell expansion and cryopreservation

Deliverables:

  • Two (2) clones that are homozygous for the targeted insertion, with two (2) vials of each clone at 1 x 10^6 cells/vial.
  • Project Milestones Reports, as well as a Final Report containing details of targeting design, experimental, and genotyping results.

Timeline: 3-4 months

Applications:

  • Physiologically relevant disease models for hard-to-model diseases (Ex. ALS, muscular dystrophy, Parkinson's disease, Alzheimer's)
  • Differentiate to study mutations in different tissue lineages with an isogenic panel of cell line models
  • Ideal for target drug discovery, drug and toxicity screening