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CRISPR/Cas9 Knock-in iPSC Service - Stem Cell Services - Services - Research

SKU :
ASC-6013-4
Catalog # :
ASC-6013-4
Questions ? Contact Us
Description
Generate stable gene knock-in iPSC lines using our acclaimed and unique expertise in CRISPR iPSC/ ES Cell Genome Editing Service:
- Send us your healthy/ patient iPSC or ESC lines; OR
- Choose from our well-characterized control “master” iPSC lines
Also available, downstream iPSC differentiation and characterization services.
- Optimized CRISPR protocols for high success rate
- Footprint-free, feeder-free transfection and iPSC culture protocols
- Isogenic control lines for reliable experiments
- Knock-in a variety of genes for different applications
- Locus-specific small/ large fragment gene insertions
- Safe harbor locus knock-in
- Reporter gene knock-in/ gene tagging
- Gene overexpression (Ex. Antibody/ antigen expression)
- Promoter modifications
- Gene replacement; gene therapy
- Gene fusion/ translocation
Workflow includes:
- Cell line evaluation
- gRNA design, CRISPR vector and donor DNA construction, and reagent validation
- Transfection of targeting vectors and optimization
- Screening for single cell clones and clone confirmation
- Cell expansion and cryopreservation
Deliverables:
- Two (2) clones that are homozygous for the targeted insertion, with two (2) vials of each clone at 1 x 10^6 cells/vial.
- Project Milestones Reports, as well as a Final Report containing details of targeting design, experimental, and genotyping results.
Timeline: 3-4 months
Applications:
- Physiologically relevant disease models for hard-to-model diseases (Ex. ALS, muscular dystrophy, Parkinson's disease, Alzheimer's)
- Differentiate to study mutations in different tissue lineages with an isogenic panel of cell line models
- Ideal for target drug discovery, drug and toxicity screening