CRISPR Knockout iPSC Service

Applied StemCell is a leader in both CRISPR genome editing and iPSC technologies. We offer a combination CRISPR iPSC/ ES Cell Genome Editing Service with both these technologies to offer. With > 500 unique iPS cell line models generated to-date and a >98% success rate, leverage our unique expertise. We can generate gene knockout in your gene of interest.

• Send us your healthy/ patient iPSC or ESC lines; OR
• Choose from our well-characterized control “master” iPSC lines

Also available, downstream iPSC differentiation and characterization services.

Other features include:

• Frameshift or target exon deletion; Large fragment deletion (>10 kb)
• Homozygous (or heterozygous) knockout clones
• Footprint-free, feeder-free transfection and iPSC culture protocols
• Isogenic control lines for reliable experiments

Workflow includes:

• Cell line evaluation
• gRNA design, construction, and validation
• Transfection and optimization
• Screening for single cell clones and clone confirmation
• Cell expansion and cryopreservation

Deliverables:

• Two (2) clones that are homozygous (or heterozygous) for the targeted null allele, with two (2) vials of each clone at 1 x 10^6 cells/vial.
• Project Milestones Reports, as well as a Final Report containing details of targeting design, experimental, and genotyping results.

Timeline: 3-4 months

Applications:

• Physiologically relevant disease models for hard-to-model diseases (Ex. ALS, muscular dystrophy, Parkinson's disease, Alzheimer's)
• Differentiate to study mutations in different tissue lineages with an isogenic panel of cell line models
• Ideal for target drug discovery, drug and toxicity screening