TARGATT™ CHO-S Master Cell Line & Knockin Kit (Academic Price)
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* MTA required
The TARGATT™ CHO-S Master Cell Line and transgenic kit was designed for fast and site-specific knockin in Chinese Hamster Ovary (CHO) cells using an easy-to-use gene knockin approach. The master cell line provided in this kit contains both the “attP” docking-site and the PhiC31 integrase expression cassette engineered into the CHO H11 safe harbor locus in the genome. Any gene of interest can be cloned into the provided TARGATT™ “attB” cloning plasmid (under control of the strong CAG promoter or promoter-of-choice), and transfected into the master cell line for generating a stable knockin cell line. The TARGATT™ integrase-based technology enables efficient DNA integration and high-level gene expression without disrupting internal genes. The TARGATT™ CHO-S cell line can therefore be used for uniform, site-specific gene knockin, generation of isogenic cell lines, and amplification strategies for isolating high expression cells, without the need for single cell cloning.
The TARGATT™ CHO-S master cell line and transgenic kit are suitable for research applications involving gene overexpression and high-level expression of recombinant proteins and other biologics in a rapidly expanding bioproduction industry and for other applications*.
Advantages of using TARGATT™ Master Cell Lines for gene knockin:
- Stable cell line generation
- Single copy, site-specific gene integration into the safe harbor locus
- High-level gene expression from an active, intergenic locus
- Use of selection marker is not necessary but optional
- Easy-to-use protocol requiring transfection of only one (donor) plasmid
*TARGATT™ master cell lines can be generated in any cell line including stem cells. Please contact Applied StemCell for TARGATT™ cell line engineering services to generate a master cell line in a specific cell line of choice.
Please also see our frequently asked question section in our webpage for more details.
This product is for research purposes only.
For industry clients, we have the following available:
- Evaluation package
- Custom antibody library construction service
Uniform and Stable GFP expression 21 days post-transfection
Figure 1. The CAG-GFP vector was used to evaluate gene integration in the TARGATT™ CHO-S master cell line. An enriched GFP signal was detected by fluorescence imaging. (Left) bright field microscopy. (Right) Immunofluorescence; GFP channel.
FACS analysis of GFP expression level in CHO-S cells
Figure 2. GFP expression was measured by fluorescence imaging after transfection in parental CHO cell lines by random integration (A-B) and in TARGATT™ CHO-S Master Cell Line by site-specific gene integration (C-E). (A) CHO-S parental cell line without transfection; (B) CHO-S parental cell line randomly transfected with GFP plasmid; (C) CHO-S master cell line without transfection; (D) CHO-S master cell line transfected with GFP plasmid before GCV selection; (E) CHO-S master cell line transfected with GFP plasmid after GCV selection.
TARGATT™ Master Cell Line Publication:
- Chi, X., Zheng, Q., Jiang, R., Chen-Tsai, R. Y., & Kong, L. J. (2019). A system for site-specific integration of transgenes in mammalian cells. PLOS ONE, 14(7), e0219842.