Knock In Mice, Genome Editing

  • Stem Cell Service

    • iPSC Generation

      Make your own isogenic cell line! Six-step service for human iPSC generation with following choices of method: retrovirus vectors, mRNA or episomal vectors. This custom cell line generation service is available for other species.

    • Stem Cell Derivation

      Mouse embryonic stem cell (ESC) derivation service from any strain. We also derive isogenic cell lines from transgenic mice.
    • Stem Cell Characterization

      Services to identify and characterize Embryonic Stem Cell (ESC) and Induced Pluripotent Stem Cell (iPSC) lines including teratoma analysis, embryoid body (EB) formation assay and pluripotency marker assay.

    • Neural Stem Cell Differentiation

      Service for ESC / iPSC differentiation into Neural Stem Cells (NSCs) , Motor Neurons and Glial Cells (Astrocytes and Oligodendrocytes).
    • Hepatocytes Differentiation

      Hepatocytes Differentiation Service from iPSC

    • Cardiomyocytes Differentiation

      Applied StemCell, Inc., provides a comprehensive range of service to differentiate iPSCs into cardiomyocyte and Hepatocytes (from healthy/ disease samples) including GMP grade iPS cells expansion and plating service.

    • Stem Cell Genome Editing

      The CRISPR/Cas9 modified induced pluripotent stem cells (iPSCs) iPSC disease modeling service can be used for in vitro genetic disease modeling.

    • Safe Harbor Locus Master iPSC Genome Editing Service

      Site-specific genetic manipulation of our TARGATT™ human iPSC line.

    • iPSC Culture Services

      Standard and customized cell culture services (FBS lot evaluation, mycoplasma testing, etc).

  • Neurotoxicity Testing

  • CRISPR/Cas9 Genome Editing

    • Cell Line Model Generation

      CRISPR Genome Editing for Cell Lines

      We offer full-service for gene editing in any mammalian cell and targeting any gene of interest. Our CRISPR cell line editing portfolio includes > 300 cell line models generated from > 100 distinct parental cell lines, including hard-to-transfect cell lines, blood lineage cell lines, cancer cell lines and stem cells.

    • Mouse Models

      Applied StemCell's comprehensive CRISPR mouse model generation service includes a broad range of gene modification portfolios suitable for all preclinical research applications, as well as phenotype analysis. Applied StemCell, Inc. has an AAALAC accredited animal facility and all mice and rats are generated within the USA.

    • Rat Models

      Applied StemCell offers full range of CRISPR/Cas9 engineered transgenic rat model generation service with a variety of genetic modifications suitable for all preclinical research applications, including research applications in the neuroscience and cancer space. All animal models are engineered in our US facility under strict regulatory protocols.

  • TARGATT™ Genome Editing

  • Lentivirus Stable Cell Line Generation Service

  • DNA Cloning / Virus Packaging Service

    • Virus Packaging (Lentivirus and Retrovirus)

       Applied StemCell Offers Custom Lentivirus and Retrovirus Packaging Service

      Lentivirus and retrovirus transfection are some of the most efficient methods of mammalian gene delivery for in vitro and in vivo applications. Applied StemCell provides lentiviral and retroviral custom virus packaging services to help advance your research without the time consuming effort of designing and preparing your own viruses and obtaining reliable and reproducible results yourself. Let Applied StemCell be your experienced experts in this field. We also provide custom virus packaging for efficient delivery of CRISPR/Cas9 components for in vivo and in vitro models.

       Some of the services we offer are:

      • Rapid custom virus packaging service for lentivirus or retrovirus virus  packaging
      • Ready-to-transduce viral particles provided back to you
      • High quality and high titer viral particles available in as few as 10 days
      • VSV-G pseudotyped viruses that exhibit broad tropism across a range of cell types
      • High titer amplification of viruses, up to 10^9 infectious units per ml, IFU/ml
      • Accurate viral titers quantified by qRT-PCR
      • Production of, over expression, inducible expression, knock down or knocked-out expression models
      • Confirmation, by DNA sequencing, that we generated the correct vector for you
      • Large scale production, clonal expansion and cryopreservation

      Lentivirus transfection is one of the most efficient techniques to deliver mammalian genes into cells and has many advantages over other gene delivery methods, such as:

    • Vector Design / Cloning Service

    • CRISPR / Cas9 Vector Design & Construction

    • Bacterial Artificial Chromosome (BAC) Design and Cloning

      Bacterial Artificial Chromosomes (BACs) are ultra-low copy vectors that can hold up to 300 kb of genomic fragments, making them ideal vectors for introduction of entire genes including the regulatory regions for disease modeling with transgenic animals.

  • Custom Services

    • Fibroblasts Immortalization Service

      Applied StemCell offers patient fibroblast immortalization service via the simian virus 40 T antigen.
    • Custom Primary Cell Isolation Service

      Applied StemCell can isolate primary cells from fresh tissues, including from biopsy samples.

    • FFPE Service

      Need a Custom FFPE Standards using your own cell line(s)?  Custom FFPE Processing of Your Cell Lines: Single or Multi-Spot Capabilities.


  • Bio Production

    • CHO Cells

      Bioproduction CHO Cells - Site-specific transgene knock-in platform using TARGATT™ CHO Master Cell Lines.

    • Transgenic Animals - Rabbit

      Bioproduction in transgenic animals - Applied StemCell's animal bioproduction platform uses transgenic TARGATT™ Rabbit to express recombinant proteins with very high yield and consistency.