CRISPR Knock-in Cell Line Service - CRISPR/Cas9 Cell Line Service - Cell Line Models - CRISPR/Cas9 Genome Editing - Services - Research

Custom knock-in stable cell line generation service using highly optimized CRISPR/Cas9 protocols. ASC has generated 1300+ unique cell line models with a wide variety of modifications. Avail some of our popular CRISPR/Cas9 knock-in cell line service for reliable, affordable and fast turnaround time for generating your cell line models.

• Locus-specific small/ large fragment gene insertions
• Safe harbor locus knock-in
• Reporter gene knock-in/ gene tagging
• Gene overexpression (Ex. Antibody/ antigen expression)
• Promoter modifications
• Gene replacement; gene therapy
• Gene fusion/ translocation

Other features include:

• Homozygous/ heterozygous knock-in clones
• Variety of cell lines: cancer, hard-to-transfect, most mammalian species

Workflow includes:

• gRNA design, construction, and validation
• Donor DNA construction
• Cell line validation, transfection and optimization
• Screening for single cell clones and clone confirmation
• Cell expansion and cryopreservation

Deliverables:

• Two (2) clones that are homozygous for the targeted insertion, with two (2) vials of each clone at 1 x 10^6 cells/vial.
• Milestone update/report; final report with detailed description of each procedure, including targeting vector design, construction and validation, transfection condition, genotyping strategy, and results.

Timeline: 3-4 months

Applications:

• Disease modeling for immuno-oncology, pharmacogenomic studies
• Drug discovery and drug efficacy and toxicity screening; drug combination studies
• Antibody validation/ screening
• Deriving diagnostic reference standards and materials
• Recombinant protein production in CHO cells
• Generation of TARGATT™ master cell lines by inserting an attP "docking site" for site-specific gene knock-in