Custom Primary Cell Isolation Service

Primary cells differ from immortalized cell lines because they have a limited population doubling time. However, primary cells retain the physiological properties of the tissue of origin and mimic in vivo conditions. Primary cells are ideal cell models for a variety of research, including cell biology/physiology, disease mechanisms, drug screening and therapeutic development.  Applied StemCell can isolate a specific cell type from tissues including (but not limited to):

  • Skin
  • Heart
  • Liver
  • Kidney
  • Bone
  • Skeletal Muscle
  • Brain or other nervous tissue

We first isolate the cell-type-of interest using magnetic bead technology. Cells are then frozen in liquid nitrogen for long-term storage. Thawed cells are subject to flow cytometry and plated for immunocytochemistry for further characterization. We also provide recommended growth and handling conditions for you with each purchase.

Case Studies

Case Study: Isolation of A2B5+ Neural Cells followed by Differentation into Oligodendrocytes.

Isolation and culture of A2B5 cells, with immunocytochemical characterization.

A2B5 cell isolation

A2B5 cell culture

FACS analysis of A2B5 cells

A2B5 cells FACS analysis

A2B5 cell characterization

A2B5 cell characterization

A2B5 cell characterization

 

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