Vector Design / Cloning Service

With decades of experience our Molecular Biology Team has developed an exceptional expertise in anything DNA-related. We  can help you develop the best strategy to tackle your cloning projects and find solutions to all your technical problems. 

  • Cloning of amplified DNA fragments
  • Gene targeting vectors for homologous recombination
  • Bacterial Artificial Chromosome (BAC) Recombineering
  • RNAi and inducible vectors
  • Site-specific mutagenesis
  • Tagging of genes
  • De novo gene synthesis

A typical cloning procedure includes:

  • Primer design for target region
  • PCR amplification of target region
  • Cloning of the PCR product into a cloning vector
  • Sub-cloning the gene into the vector of your choice
  • Picking, plating, culturing, and DNA preparation
  • Sequence confirmation
  • Plasmid purification (Mini, Midi, or Maxi size)
References

Applied StemCell's citations and publications in journals:

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