References

Applied StemCell References

 

Publications (* Cited Applied StemCell)

SERVICE

CELL LINES: Genome Editing Services for Cell Lines

CRISPR Human Blood Lineage Cell Lines (Jurkat and TF-1)

  • POSTER, Tricon Conference 2016, San Francisco, CA (March 10-11, 2016)

CRISPR Cell Line Knock-Out, Knock-in, Point Mutation

TARGATT™ Site-Specific Knock-in Cell Line

Description of the technology

Commentary, comparison with other transgenic methods

Tet inducible mice generated by TARGATT™

Advantage of Hipp11 (H11) locus

Application for mice generated by TARGATT™

 

MOUSE MODELS: Knock-in Mouse, Knockout Mouse Models

TARGATT™ Site Specific Knock-in Mouse 

Description of the technology

Commentary, comparison with other transgenic methods

Tet inducible mice generated by TARGATT™

Advantage of Hipp11 (H11) locus

Application for mice generated by TARGATT™

CRISPR Knock-in, CRISPR Knockout Mouse (*cited/published articles)

 CRISPR Technology

CRISPR Knock-in H11 Locus in Pigs

 Knock-in, Knockout, Conditional Knock-out

Homologous Recombination Conditional Knockout Mouse (*cited/published articles)

 

RAT MODELS: Knock-in Rat Models, Conditional Knockout Rat

TARGATT™ Site-Specific Knock-in Rat H11

Description of the technology

Commentary, comparison with other transgenic methods

Tet inducible mice generated by TARGATT™

Advantage of Hipp11 (H11) locus

Applications for animal models generated by TARGATT™

CRISPR Knock-In, Knock-Out, Conditional Knock-Out Rat

CRISPR Technology

CRISPR Knock-in H11 Locus in Pigs

Others

 

iPSC services

Safe Harbor Locus Master iPSC Generation with TARGATT™

iPSC Disease Modeling

Teratoma Analysis, iPSC Characterization

Teratoma Formation Assay (Published reports of ASC's Teratoma Formation Analysis Reports can be found in the Certificate of Analysis "Induced Pluripotent Stem Cells (iPSCs)" by Coriell Institute for Medical Research.)

References (*cited/published articles)

 Karyotyping (*cited/published articles)

 

DNA Cloning/ Virus Packaging Service

Vector Design/ Cloning Service (*cited/published articles)

Lentivirus Packaging

 

PRODUCTS

Cell Line Generation: Genome Editing Products

CRISPR Point Mutation, Knockin, Knockout Kit

CRISPR Cas9 Expressing Cell Lines

  •  Poster, Tricon Conference 2016, San Francisco, CA (March 10-11, 2016).

CRISPR Safe Harbor Locus Knock-in Kit (Human, Mouse Cells)

CRISPR

AAVS1 locus

  • Varshney, GK., et al. (2015). Genome research, 25(7), 1030-1042.
  • Tiyaboonchai, A., et al. (2014) Stem cell research, 12(3), 630-637
  • Sadelain, M., et al. (2012). Cancer, 12(1), 51-58.

H11 locus

Rosa26 locus

TARGATT™ Safe Harbor Locus Knock-in Kit (Mouse)

Description of the technology

Commentary, comparison with other transgenic methods

Tet inducible mice generated by TARGATT™

Advantage of Hipp11 (H11) locus

Applications for mice generated by TARGATT™

CRISPR Cas9 GeCKO Library


Genome Editing Kits for Knock-in, Knockout, CKO Mouse generation

Custom Mouse Injection-Ready Kit (Validated)

in vivo validated Cas9 mRNAs

 

TARGATT™ Products for Site-Specific Knock-in Mouse Generation

TARGATT™ Embryos/ Mice, Plasmids, Transgenic Kits, Genotyping Kits

Description of the technology

Commentary, comparison with other transgenic methods

Tet inducible mice generated by TARGATT™

Advantage of Hipp11 (H11) locus

Applications for mice generated by TARGATT™

 

Genome Editing Kits for Knock-in, Knockout, CKO Rat generation

 CRISPRCRITTERS™ Custom Rat Injection-Ready Kit (Validated)


 Patient Samples, Cell Pellets, Immortalized Fibroblasts

 Human Primary Cell (Normal)

 

FFPE Reference Standards

MAPK Mutation Panel FFPE Reference Standards

  •  Akinleye A, Furqan M, Mukhi N, Ravella P, Liu D (2013) MEK and the inhibitors: from bench to bedside. J. Hematol. Oncol. 6, 1–11
  • Santarpia L, Lippman SM, El-Naggar AK (2012) Targeting the MAPK–RAS–RAF signaling pathway in cancer therapy, Expert Opin. Ther. Targets 16, 103–119
  • Relling MV, Evans WE (2015) Pharmacogenomics in the clinic, Nature 526, 343–350

 

Cell Culture

2D3D Cell Culture MyEZGel™ for Cancer and other cell lines

  • Huang, H., et al. (2013). PLoS One, 8(3), e59482.
  • Kumar, D., et al. (2015). JAMA Otolaryngology–Head & Neck Surgery, 141(12), 1133-1139.
  • Li, X., et al. (2013). Vaccine,31(41), 4508-4515.
  • Huang H., et al. (2011). Soft Matter.  7 (19):8905 – 8912.

 ESC-Sure™ FBS

MEF Feeder Cells

DR4 MEF Feeder Cells (*cited/published articles)

CF-1 MEF Feeder Cells (*cited/published articles)

Neo-resistant MEF Feeder Cells (*cited/published articles)

SNL 76/7 (STO Cell Line)

Medium

ESC-Sure™ Serum-/Feeder- Free hESC/iPSC Culture Medium (SFFM)-  *cited/published articles

  • Szabo, AZ., et al. (2013) Stem Cells. 31(4):786-99

ESC-Sure™ mESC Complete Medium (*cited/published articles)

  • Okada, A., et al. (2016) Embryonic Stem Cell Protocols, 181-195.

ESC-Sure™ Basal Medium Mouse DMEM

Cytokines (*cited/published articles)

 

Stem Cell Research

 iPSC/ESCs

 Human iPSCs: iPSCs from ALS8 patient and non-carrier siblings

  •  Mitne-Neto M, Machado-Costa M, Marchetto MCN, et al. Downregulation of VAPB expression in motor neurons derived from induced pluripotent stem cells of ALS8 patients. Human Molecular Genetics. 2011;20(18):3642-3652. doi:10.1093/hmg/ddr284.

 Mouse/ Rat/ Pig/ Guinea Pig


Stem Cell Characterization

Pluripotency Markers (Protein)

  • Anti-SOX2 antibody:
    • Wang, T., et al. (2015) J. Vis. Exp. (95), e52298, doi:10.3791/52298 (2015)

 

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