TARGATT™ HEK293 Kits

Accelerate cell line development for library creation and protein expression with efficient TARGATT™ large knock-in technology

DNA insertion simplified

From promoter screening libraries to protein expression and stable viral vector production, TARGATT™ HEK293 Kits put efficient and precise cell line development into your hands.

Leveraging the power of site-specific integration and the consistently-expressing H11 safe harbor site, our TARGATT™ large knock-in technology enables genome engineering that CRISPR, transposons, and lentivirus can’t handle.

Expand what you can accomplish in HEK293 cells with TARGATT™ technology

Available TARGATT™ HEK293 Kits

TARGATT™ Kits are designed to enable easy evaluation of the technology before purchasing a license*. Each TARGATT™ HEK293 Kit includes a HEK293 cell line with the TARGATT™ landing pad already inserted into the H11 safe harbor site, a donor plasmid for the DNA you are knocking in, a positive control plasmid, and a TARGATT™ Integrase expression plasmid.

*Academic researchers may purchase TARGATT™ technology without licensing. Request more information.

Cat. #	Product Name	Price
AST-1305	TARGATT™ HEK293 Knock-in Kit (H11)	Inquire
AST-1306	TARGATT™ HEK293 Knock-in Kit (H11) for Library Screening (Drug Selection)	Inquire
AST-1308	TARGATT HEK293 High-Efficiency Sub-Line (HESL) Knock-in Kit (H11) for Library Screening (FACS)	Inquire

If you are interested in outsourcing genome engineering, contact us for a custom service project quote.

Why you should choose TARGATT™ HEK293 Kits

Efficient integration—over 90% after selection
Consistent expression from clone to clone, enabling the use of pools instead of clonal purification
Large cargo knock-in—up to 20 kb in a single reaction, more with nested reactions
Site-specific integration into the H11 safe harbor site
Single-copy insertion

Learn how TARGATT™ technology works and see the data—visit the technology page.

Because integration using TARGATT™ technology is so efficient, you can create highly diverse libraries and even perform mammalian display for screening in a biologically-relevant cell type.

See our work in action

A high throughput bispecific antibody discovery pipeline

Segaliny AI, Jayaraman J, Chen X, et al.
Communications Biology. 2023;6(1). doi:10.1038/s42003-023-04746-w

Need more information?

Fill out the contact form below and a team member will be in touch within one business day.

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Publications about TARGATT™ technology

Chi, X., Zheng, Q., Jiang, R., Chen-Tsai, R. Y., & Kong, L. J. (2019). A system for site-specific integration of transgenes in mammalian cells. PLOS ONE, 14(7), e0219842.
Zhu, F., Gamboa, M., Farruggio, A. P., Hippenmeyer, S., Tasic, B., Schüle, B., … Calos, M. P. (2014). DICE, an efficient system for iterative genomic editing in human pluripotent stem cells. Nucleic Acids Research, 42(5), e34. http://doi.org/10.1093/nar/gkt1290.