TARGATT™ Rosa26 Mouse Genotyping Kit

SKU :
AST-2006
Catalog # :
AST-2006
Size :
1 Kit (100 reactions)
$220.00
Overview

The TARGATT™ Mouse Genotyping Kit allows for convenient genotyping of TARGATT™ Transgenic Mice via tail biopsy. The kit contains reagents, PCR primer sets, buffer along with detailed protocols and anticipated results. This kit is intended for use after TARGATT™ Transgenic mouse model generation using the TARGATT™ Transgenic KitTARGATT™ Plasmids, and TARGATT™ “attP” Mice to confirm the transgene integration into the TARGATT™ attP docking site at either the mHipp11 (mH11) or mRosa26 locus.

Contents:

AST-2005: The TARGATT™ H11P3 Mouse Genotyping kit contains:

  • Tail Lysis Buffer
  • Proteinase K
  • Primer Set H11P3
  • Primer Set SSL
  • Primer Set SSR
  • Nuclease-free water
  • Positive control DNA

The general protocol involves extraction of genomic DNA from tail samples followed by genotyping by PCR using the provided primers. The kit allows you to detect presence of the H11 attP knock-in site, and integration of the transgene at both the attL and attR sites.

AST-2006: The TARGATT™ Rosa26P3 Mouse Genotyping kit contains:

  • Tail Lysis Buffer
  • Proteinase K
  • Primer Set Rosa26P3
  • Primer Set SSL
  • Primer Set SSR
  • Nuclease-free water
  • Positive control DNA

The general protocol involves extraction of genomic DNA from tail samples followed by genotyping by PCR using the provided primers. The kit allows you to detect presence of the Rosa26 attP knock-in site, and integration of the transgene at both the attL and attR sites.

Resources:

  • Watch our "How-to Guide" video for a brief overview of how to generate your own transgenic knock-in mouse model using the TARGATT™ technology.
  • We also provide custom service where we can do the microinjection for you and send you the mice. Please see our TARGATT™ Services.