Teratoma Formation Analysis

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Service to confirm pluripotency of human and mouse ESC and iPSC lines by Teratoma Formation Analysis after injection of cells into an animal.


  • Turnaround time: 4 weeks (Mouse), 7 weeks (Human)
  • Number of cells required / injection site: Mouse ESCs/iPSCs = 0.5 x 10^6,  Human ESCs/iPSCs =  1 x 10^6
  • Enhanced experimental protocol: Injection under both the kidney capsule and in testis results in higher efficiency of distinct teratoma formations with differentiated tissues derived from all three germ layers.

Service Includes:

  • Comprehensive report with histological analysis and high resolution publication-grade images of endoderm, mesoderm and ectoderm formation.
  • Tissue blocks and H&E stained tissue section slides


  • Frozen sample culture Fee $895 per line (No fee for fresh cells)
  • Pathogen tests (please provide mycoplasma test result showing negative; otherwise additional testing fee will be required.) 
  • Kidney capsule tumor and testis tumor from each mouse will be embedded in one block. Separate blocks are available upon request with additional fee.
  • Frozen tissue blocks are available with additional fee.


Technical Details:

  • Experimental setup: A total of 4 mice (3 experimental and 1 control) will be used.
  • Injection site: Each recipient mouse will be injected at two locations: in the kidney capsule and in the testis.
  • Service includes: Cell injection, teratoma harvesting, tissue preparation (embedding and sectioning), H&E staining and histology analysis of teratoma tissue sections.


High Teratoma Formation Rate:


Published reports of ASC's Teratoma Formation Analysis Reports can be found in the Certificate of Analysis "Induced Pluripotent Stem Cells (iPSCs)" by Coriell Institute for Medical Research.


Requsition Form


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