Safe Harbor Locus Master iPSC Genome Editing Service

 Safe Harbor Locus Master iPSC Generation with TARGATT™

TARGATT™ Master Human iPSC line

The TARGATT™ master iPSC line contains a "docking attP" site at a safe harbor genomic Rosa26 locus. Any genes of interest on an attB vector can be inserted efficiently at the Rosa26 locus through phiC31 integrase mediated recombination between attP and attB. Efficiency of transgene insertion is up to 100% with drug selection and up to 30% without drug selection.

Application:

  • iPSC reporter lines for cell tracking
  • iPSC lines with surface markers for cell purification
  • iPSC lines with cell-specific promoters to direct iPSC differentiation

Projects:

  • iPSC lines with excisable Cas9 or nickase for efficient CRISPR gene modification
  • High-efficiency, high purity cells differentiated from iPSC lines

iPSC lines with cell-specific promoters driving (1) cell surface to induce differentiation of specific cell lineages; (2) cell-type specific transcription factors to induce differentiation of specific cell lineages; (3) reporters to track cell differentiation process.

Example Data:
ASC engineered: Site-Specific Knock-in of TARGATT™ sites, with mCherry reporter (left). Original Cell Line: hiPSC (right).
TARGATT-iPS-mCherry-1 TARGATT-iPS-mCherry-2
 
Applied StemCell can also create Site-Specific Knock-in cell lines for primary cells or other cell lines.

 

Support Materials
  • Poster: ISSCR 2016. "Neuronal Differentiation and Genome Editing in Human iPSCs". Poster #W2087. Ruby Yanru Chen-Tsai, Robert Diaz, Jimmy Tai, Lin Yan, Lingjie Kong, Applied StemCell, Inc., Milpitas, CA 95035, U.S.
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