Neo-resistant MEF Feeder Cells
Isolated from mouse embryos and are resistant to neomycin.
MEF cells serve as feeder cells to support the growth of undifferentiated mouse or human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). MEF cells are isolated from mouse embryos and should be used at an early passage. Before use as feeder cells, MEF cells must be mitotically inactivated by γ-irradiation or mitomycin-C treatment.
Neo-resistant MEF feeder cells are derived from 13.5 day old mouse embryos that are genetically engineered to contain Neomycin-resistance genes and are optimized for the culture of ES/iPS cells under G418 selection.
All MEF cells are manufactured in the US and have an average viability of 95% after thawing. We offer both untreated cells for further expansion and treated cells that can be directly used as a feeder layer.
Applied StemCell's cited and published journal articles:
- Mansour, A. A., Gonçalves, J. T., Bloyd, C. W., Li, H., Fernandes, S., Quang, D., ... & Gage, F. H. (2018). An in vivo model of functional and vascularized human brain organoids. Nature biotechnology, 36(5), 432. doi:10.1038/nbt.4127
- Heim, CN. (2012) Biol Reprod. 87(4), 90.
- Mauney, Joshua R (2010) PLoS ONE 5(7): e11513.