Motor & Cortical neuron Differentiation
Motor neurons are a type of neuron located within the spinal cord and brain, that project axons to the spinal cord or to the peripheral effector organs such as muscle and glands, to directly or indirectly control their activity. Damage to motor neuron and the motor circuits results in many devastating motor neuron diseases (MNDs) that encompasses a variety of neurological disorders that affect voluntary muscle movement. Some common MNDs include Amyotrophic Lateral Sclerosis (ALS) and Spinal Muscular Atrophy (SMA). There is no cure or effective treatment for these diseases, and treatment is only symptomatic or supportive. A serious bottleneck in identifying a cure, delaying disease progression or reversal of neuronal damage, is due to a lack of understanding of the disease, which in turn is due to a dearth of a reliable source of functional disease models. The emergence of iPSC technology and its further differentiation into post-mitotic somatic cell lineages, especially to neural stem cells and neurons such as motor neurons, has provided MND researchers with an unlimited supply of physiologically and phenotypically relevant in vitro disease models.
Applied StemCell offers custom iPSC-to-motor and cortical neuron differentiation services for your neuroscience research needs. Our optimized protocols enable efficient, integration-free generation of high-quality and purity motor or cortical neurons that exhibit typical biomarkers and functionality.
- High-purity iPSC-derived motor neurons or cortical neurons
- Express typical biomarkers
- Generate motor/cortical neurons from your patient's iPSC samples (healthy/ disease) or engineered iPSCs.
- Fast turnaround time
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Are you in need of iPSCs to genetically modify and further differentiate into motor or cortical neurons? We offer fully customizable iPSC generation from human or non-human tissue samples, and our genome editing experts can use CRISPR or TARGATT™ to genetically alter the iPSCs to fit your project requirements. If you need to expedite your experimental timeline, ASC also offers off-the-shelf motor and cortical neurons. Contact us today to find the best solution for your immunotherapy projects.
Products and Services
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Case Study 1
Motor Neuron Differentiation from Applied StemCell's Master iPSC Line, ASE-9211
Control iPSC line, ASE-9211 was used for differentiation into motor neurons using proprietary, integration-free protocols.
Figure 1. Immunocytochemical staining images of the motor neuron derived from control "master" iPSC line, ASE-9211 at 2 days post thaw. The iPSC-derived motor neurons were stained with antibodies for motor neuron biomarker, HB9 (green), and neuronal biomarker, Tuj1 (red). DAPI (blue) was used as nucleus stain.
Figure 2. Immunocytochemical staining for motor neuron marker, ChAT (green) and neuronal marker, MAP2 (red) in motor neurons differentiated from control "master" iPSC line, ASE-9211 at 7 days post thaw. DAPI (blue) was used for nucleus staining.
Case Study 2:
Characterization of iPSC-derived Cortical Neurons (ASE-9741)
Figure 1. Immunostaining of ASE-9741 iPSC-derived Cortical Neurons for a cortical neuron biomarker. Cryopreserved cortical neurons, differentiated from Applied StemCell’s control iPSC line ASE-9211, were stained with Tuj1. (Red: Tuj1; Blue: DAPI)