Motor/Cortical Neuron Differentiation
Applied StemCell offers custom iPSC-to-motor neuron differentiation service for your neuroscience research needs. Our proprietary protocols enable very efficient, integration-free generation of high-quality and purity motor neurons that exhibit typical biomarkers (HB9, ChAT, and more) and functionality.
- Generate motor neurons from your patient iPSC samples (healthy/ disease) or engineered iPSCs.
- Provided as cryopreserved, ready-to-use neurons.
- We also offer iPSC generation service for your patient samples; or you can use our master control iPSC lines with proven motor neuron differentiation and CRISPR engineering capabilities.
Make isogenic neuronal panels with differentiation to other neuronal subtypes and glial cells (astrocytes, oligodendrocytes, and microglia)
Motor Neuron Differentiation from Applied StemCell's Master iPSC Line, ASE-9211
Control iPSC line, ASE-9211 was used for differentiation into motor neurons using proprietary, integration-free protocols.
Figure 1. Immunocytochemical staining images of the motor neuron derived from control "master" iPSC line, ASE-9211 at 2 days post thaw. The iPSC-derived motor neurons were stained with antibodies for motor neuron biomarker, HB9 (green), and neuronal biomarker, Tuj1 (red). DAPI (blue) was used as nucleus stain.
Figure 2. Immunocytochemical staining for motor neuron marker, ChAT (green) and neuronal marker, MAP2 (red) in motor neurons differentiated from control "master" iPSC line, ASE-9211 at 7 days post thaw. DAPI (blue) was used for nucleus staining.
Motor neurons are a type of neuron located within the spinal cord and brain, that project axons to the spinal cord or to the peripheral effector organs such as muscle and glands, to directly or indirectly control their activity. Damage to motor neuron and the motor circuits results in many devastating motor neuron diseases (MNDs) that encompasses a variety of neurological disorders that affect voluntary muscle movement. Some common MNDs include Amyotrophic Lateral Sclerosis (ALS) and Spinal Muscular Atrophy (SMA). There is no cure or effective treatment for these diseases, and treatment is only symptomatic or supportive. A serious bottleneck in identifying a cure, delaying disease progression or reversal of neuronal damage, is due to a lack of understanding of the disease, which in turn is due to a dearth of reliable source of functional disease models. The emergence of iPSC technology and its further differentiation into post-mitotic somatic cell lineages, especially to neural stem cells and neurons such as motor neurons, has provided MND researchers with an unlimited supply of physiologically and phenotypically relevant in vitro disease models.
Applied StemCell provides customized service to differentiation neural stem cells and motor neurons from your patient iPSC lines.
- Derive motor neurons from control, disease, or engineered iPSCs
- High purity, iPSC-derived motor neuron progenitors (MNPs) or functionally mature cells, including detailed maturation and maintenance protocols.
- Express typical motor neuron markers: HB9, ChAT (other markers available as an option)
- Engineer disease phenotypes in our master iPSC lines using our acclaimed CRISPR-iPSC engineering services, and then differentiate to motor neurons.
- Leverage our control “master” iPSC lines to derive motor neurons derived to use as experimental controls for your disease or engineered lines
- Media with supplements provided along with the cells.
- Provided as cryopreserved, ready-to-use neurons are provided
You can provide either your own iPSC lines or can use Applied StemCell’s well-characterized, master iPSC lines that are amenable to CRISPR genome editing and directed-differentiation to motor neurons.
We can generate iPSCs from your patient samples: PBMCs, skin fibroblasts, urine, HSCs, adipocytes, and more.
Need iPSC-derived cortical neurons? ASC can also differentiate iPSCs from various sources into cortical neurons which are ideal for modeling neurological diseases such as Alzheimer’s disease, autism, epilepsy and stroke.
Cortical neuron markers: Tuj-1, Map2, vGlut1, NeuN
Motor Neurons / Cortical Neurons Differentiation Service
|1. Recovery, Expansion and Validation of iPSCs/ESCs||1-2 weeks||Biweekly updates throughout service|
|2. Differentiation to Motor Neuron Precursors||2-4 weeks|
|Characterization and QC of Motor Neuron Precursors (HB9, Tuj1 biomarker staining; purity, cell number, viability, mycoplasma)||1 week||2 x 10^6 cells/ vial|