• TARGATT™ Rabbit for Biopharming
    & Production
    • Single Copy Insertion At A Safe Harbor Locus
    • High-Yield Protein Production
    TARGATT™ Rabbit for Biopharming</br>& Production

Transgenic Animals-Rabbit

TARGATT™ Rabbit for Biopharming and High-Yield Recombinant Protein and Antibody Production

Animal bioproduction uses transgenic animal mammary gland as a bioreactor for the production of recombinant proteins. Animal bioproduction, compared to CHO bioproduction, has the advantages of lower upfront and maintenance costs; is easy to contain, control and transport; involves faster development processes and no scale-up issues; and has a unique low-cost bulk holding stage (frozen milk). However, existing technological methods such as random integration to create transgenic animals have many limitations. Applied StemCell has overcome these limitations with our proprietary TARGATT™ technology to reproducibly, and consistently, control the knock-in locus, copy number, and expression of the target protein in transgenic animals.

Advantages of Animal Bioproduction (Biopharming):

  • Very low upfront investment and low maintenance costs (~10 times lower than CHO)
  • Easy to contain, control and transport
  • Speed of development and expansion
  • No up-scaling problems, size of individual bioreactor constant
  • Technically less demanding
  • Allows complex protein production
  • Unique early low-cost bulk holding stage (frozen milk)
  • Easy protein isolation and purification

We are excited to use our TARGATT™technology to rapidly engineer your bioproduction animals with your gene of interest. Please contact us for details, pricing, and timelines for your specific project.


Products and Services
Technical Details

Advantages of Applied StemCell’s TARGATT™ Technology Over Existing Methods To Create Transgenic Animals.

Existing Challenges

TARGATT™ Solution

Random insertion, no control of insertion locus or copy number

Control of locus and copy number using targeted knock-in

Low yield of recombinant protein: 1-2 g/L for old method

High yield of recombinant protein by using highly expressing locus b-casein

Inconsistent from one protein to another

Consistent protein production using a high efficiency and highly reproducible knock-in strategy

Applied StemCell’s TARGATT™ technology utilizes the PhiC31 integrase and attP docking sites to direct the chosen target protein to a pre-selected and well-characterized locus, with high efficiency. Our method ensures high expression and eliminates position effects such as gene silencing, genomic instability and interruption of endogenous genes.

Case Studies

Case Study 1

Bioproduction Using TARGATT™ Rabbits

Expression of TdTomato fluorescent protein in rabbit milk


Figure 1. TdTomato expression in TARGATT™ Bioproduction Rabbit. TARGATT™ rabbit was engineered to express the 56 kDa tdTomato protein from the endogenous beta-casein locus. The yield of tdTomato protein was > 5g/L from a heterozygous rabbit (red color is due to TdTomato expression) as compared to 26 kDa rabbit beta-casein protein from a wild type rabbit.