TARGATT™ Genome Editing
Applied StemCell’s proprietary TARGATT™ technology, enables site-specific, stable integration of large DNA fragments into a safe harbour locus more efficiently and faster, with guaranteed transgene expression. The TARGATT™ gene-editing platform is versatile and can generate large fragment knock-in animal or cell line models. This technology circumvents problems associated with random integration such as position effect, and gene silencing or instability due to integration of multiple copies of the transgene. Applications for TARGATT™ models: Transgene overexpression models, reporter gene knock-in, conditional knock-in, inducible expression, Cre-driver lines and humanized animal models.
TARGATT™ Genome Editing Categories
TARGATT™ Site Specific
TARGATT™ Site-Specific
Knock-in Rat
TARGATT™ Site-Specific
Knock-in Cell Line Service
TARGATT™ High Resolution Protein Screening
Products and Services
Supporting Material
Brochures/ Flyers:
Posters:
Webinars:
How-to Guide for TARGATT™ Transgenic Kit: Dr Ruby Chen-Tsai- Choosing the Right Genome Editing Technology for Your Mouse Models: CRISPR, TARGATT™ and Beyond...: Dr. Carlisle Landel
- TARGATT™: A Mammalian System for Antibody/ Protein Library Screening (October 2018)
FAQs
Can I create models to overexpress a gene of interest?
Can I use TARGATT™ system to create transgenic models with tissue-specific gene expression?
What promoters are used to drive gene expression?
What is the specific site that my gene of interest will be integrated into?
Besides H11 and Rosa26, can gene be inserted at other loci?
Can I integrate a reporter gene? What kind of reporter genes do you recommend?
What is the maximum size of a gene you can insert? Will the efficiency of your system be affected if the gene is too large?
Why does the TARGATT™ knock-in system have high efficiency?
How many copies of the gene will be inserted into the genome?
Do you have TARGATT™ technology available for Knock-in cell lines?
Technical Details
Advantages of TARGATT™ Technology:
- High integration efficiency (up to 40%)
- Large transgene knock-in (up to 22 kb)
- Reduced time and cost
- Guaranteed, high level expression of the transgene
- Site-specificity allows a precise comparison of the effects of the transgenes among different lines
- Site-specific knock-in at pre-selected locus overcomes challenges associates with random integration:
- Eliminates position effect
- Integration at intergenic region ensures that no internal genes are interrupted
- Single copy gene integration eliminates repeat-induced gene silencing and genomic instability
Choosing the right genome editing technology: Applied StemCell uses two complementary genome editing technologies to generate advanced cell line and animal models very efficiently and effectively: the CRISPR/Cas9 technology and our propriety site-specific gene integration technology, TARGATT™ for large fragment (up to 20 kb) knock-in into a safe harbor locus.
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Project Purpose |
CRISPR/Cas9 |
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Knock-Out (KO) |
Yes |
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Point Mutation |
Yes |
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Conditional KO |
Yes |
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Knock-In (<200 Nucleotide ssODN Donor) |
Yes |
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Knock-In Transgenes in Safe Harbor Loci (>2kb) |
Challenging (but limitations on size) |
Yes (up to 20kb) |
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Knock-In (Plasmid DNA) |
Challenging (but limitations on size) |
Yes (2 steps: KI docking site; KI transgene) |
