• TARGATT™ Genome Editing

TARGATT™ Genome Editing

Applied StemCell’s proprietary TARGATT™ technology, enables site-specific, stable integration of large DNA fragments into a safe harbour locus more efficiently and faster, with guaranteed transgene expression. The TARGATT™ gene-editing platform is versatile and can generate large fragment knock-in animal or cell line models. This technology circumvents problems associated with random integration such as position effect, and gene silencing or instability due to integration of multiple copies of the transgene. Applications for TARGATT™ models: Transgene overexpression models, reporter gene knock-in, conditional knock-in, inducible expression, Cre-driver lines and humanized animal models.

TARGATT™ Genome Editing Categories

TARGATT™ Site Specific
Knock-in Mouse

Fast and Reliable! Site-specific knock-in mouse model generation service for large transgene insertion.

TARGATT™ Site Specific
Knock-in Mouse

TARGATT™ Site-Specific
Knock-in Rat

TARGATT™ Site-Specific Knock-in Rat (H11) model generation service for large fragment gene insertion into Sprague-Dawley rats.

TARGATT™ Site-Specific
Knock-in Rat

TARGATT™ Site-Specific
Knock-in Cell Line Service

Efficient generation of stable, site-specific, gene knock-in in mammalian cell lines and stem cells.

TARGATT™ Site-Specific
Knock-in Cell Line Service

TARGATT™ High Resolution Protein Screening

Site-specific, isogenic cell libraries for consistent expression of gene/protein variants for genome-wide screening and protein evolution.

TARGATT™ High Resolution Protein Screening

Products and Services

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FAQs
Can I create models to overexpress a gene of interest?
Can I use TARGATT™ system to create transgenic models with tissue-specific gene expression?
What promoters are used to drive gene expression?
What is the specific site that my gene of interest will be integrated into?
Besides H11 and Rosa26, can gene be inserted at other loci?
Can I integrate a reporter gene? What kind of reporter genes do you recommend?
What is the maximum size of a gene you can insert? Will the efficiency of your system be affected if the gene is too large?
Why does the TARGATT™ knock-in system have high efficiency?
How many copies of the gene will be inserted into the genome?
Do you have TARGATT™ technology available for Knock-in cell lines?
Technical Details

Advantages of TARGATT™ Technology:

  • High integration efficiency (up to 40%)
  • Large transgene knock-in (up to 22 kb)
  • Reduced time and cost
  • Guaranteed, high level expression of the transgene
  • Site-specificity allows a precise comparison of the effects of the transgenes among different lines
  • Site-specific knock-in at pre-selected locus overcomes challenges associates with random integration:
  • Eliminates position effect
  • Integration at intergenic region ensures that no internal genes are interrupted
  • Single copy gene integration eliminates repeat-induced gene silencing and genomic instability


Choosing the right genome editing technology: Applied StemCell uses two complementary genome editing technologies to generate advanced cell line and animal models very efficiently and effectively: the CRISPR/Cas9 technology and our propriety site-specific gene integration technology, TARGATT™ for large fragment (up to 20 kb) knock-in into a safe harbor locus.

Project Purpose

CRISPR/Cas9

TARGATT™

Knock-Out (KO)

Yes

 

Point Mutation

Yes

 

Conditional KO

Yes

 

Knock-In

(<200 Nucleotide ssODN Donor)

Yes

 

Knock-In Transgenes in

Safe Harbor Loci (>2kb)

Challenging

(but limitations on size)

Yes

 (up to 20kb)

Knock-In

 (Plasmid DNA)

Challenging

(but limitations on size)

Yes

 (2 steps: KI docking site; KI transgene) 

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!