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iPSC Generation From Human Samples
Custom iPS Cell Generation
iPSC generation is a complex process that reprograms adult somatic cells into a pluripotent, embryonic stem cell-like stage. iPSCs are typically reprogrammed by introducing products of specific sets of pluripotency-associated genes, or "reprogramming factors", called Yamanaka factors, into a given cell type. It requires extensive technical expertise to generate cells that are robust, karyotype-normal, and pluripotent. With >13 years of expertise in stem cell technologies and as a leading provider of iPSC services, we can generate iPSCs safely and efficiently in 2-3 months:
- Experienced working with Healthy/disease samples
- Reprogramming from a variety of starting tissues: blood samples, PBMCs, fibroblasts, cord blood, HSC, MSC, adipose tissue, urine, and more
- Footprint-free reprogramming with feeder-free culture conditions or virus-based
- Highly optimized, high-efficiency protocols ideal for iPSC banking/iPSC repositories (approx 95% success rate)
- iPSC Characterization for morphology and pluripotency markers
- Fast Turnaround: 2-3 months
- Add-On Services: G-banding, RT-PCR, STR profiling, and directed differentiation
- GMP iPSC Generation Available >> Learn More
Expanded characterization services are available for more advanced profiling of your iPSC lines. ASC can even genetically engineer your iPSCs using CRISPR/Cas9 or TARGATT™ and differentiate the iPSCs to the somatic lineage of your choice, including NK cells, T cells, astrocytes, cardiomyocytes, and more.
Products and Services
Publications
- Jang, Y., Choi, J., Park, N., Kang, J., Kim, M., Kim, Y., & Ju, J. H. (2019). Development of immunocompatible pluripotent stem cells via CRISPR-based human leukocyte antigen engineering. Experimental & Molecular Medicine, 51(1), 3.
- Ilic, D. (2019). Latest developments in the field of stem cell research and regenerative medicine compiled from publicly available information and press releases from nonacademic institutions in October 2018. Regenerative medicine, 14(2), 85-92.
- Allende, M. L., Cook, E. K., Larman, B. C., Nugent, A., Brady, J. M., Golebiowski, D., ... & Proia, R. L. (2018). Cerebral organoids derived from Sandhoff disease induced pluripotent stem cells exhibit impaired neurodifferentiation. Journal of Lipid Research, jlr-M081323.
- Field, A. R., Jacobs, F. M., Fiddes, I. T., Phillips, A. P., Reyes-Ortiz, A. M., LaMontagne, E., ... & Hauessler, M. (2019). Structurally Conserved Primate LncRNAs Are Transiently Expressed during Human Cortical Differentiation and Influence Cell-Type-Specific Genes. Stem cell reports.
FAQs
Do you provide thawing and splitting/passaging protocols?
Do you provide an iPSC cryopreservation protocol?
What QC testing do you conduct on the human iPSCs?