• Stem Cell Culture : MEF cells, 3D Media, FBS

MEF Feeder Cells

We offer multiple lines including: CF-1DR4, Neo-resistant and SNL (STO) feeder cells. All MEF cells are manufactured in the US and have an average viability of 95% after thawing. We offer both untreated cells for further expansion and treated cells that can be directly used as a feeder layer.

MEF Cells Categories
 

CF-1 MEF Feeder Cells

Derived from CF-1 mouse embryos; and used as feeder cells to support ESC and iPSC cultures.

CF-1 MEF Feeder Cells

DR4 MEF Feeder Cells

Derived from DR4 mouse embryos and are resistant to Neomycin, Hygromycin, Puromycin and 6-thioguanine.

DR4 MEF Feeder Cells

Neo-resistant MEF Feeder Cells

Neo-resistant MEF Feeders are isolated from mouse embryos that are genetically engineered to contain neomycin-resistance genes.

Neo-resistant MEF Feeder Cells

SNL 76/7 (STO Cell Line)

The SNL 76/7 cell line is derived from a mouse fibroblast STO cell line transformed with neomycin resistance and murine LIF genes.

SNL 76/7 (STO Cell Line)

Products and Services
Catalog ID#Product Name SizePriceQTY
$48.00
$226.00
$269.00
$189.00
$515.00
$126.00
0.00
$126.00
$578.00
$74.00
$268.00
$48.00
$37.00
$48.00
$226.00
$48.00
$226.00
$95.00
$95.00
$95.00
$268.00
$48.00
$48.00
$578.00
$226.00
$226.00
$37.00
$37.00
$74.00
0.00
$342.00
$269.00
$269.00
$24.00
$226.00
$37.00
$342.00
$269.00
0.00
0.00
0.00

41 Items

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Supporting Material

Brochures/ Flyers:

Publications

CF-1 MEF Feeder Cells

DR4 MEF Feeder Cells 

  • Tan, C. E. H. (2018). Establishing a genetically engineered mouse ES cell line expressing an inducible Xist transgene along chromosome 19 (Doctoral dissertation)

  • Fogarty, N. M., McCarthy, A., Snijders, K. E., Powell, B. E., Kubikova, N., Blakeley, P., ... & Maciulyte, V. (2017). Genome editing reveals a role for OCT4 in human embryogenesis. Nature, 550(7674), 67-73.

  • Molokanova, O., Schönig, K., Weng, S. Y., Wang, X., Bros, M., Diken, M., ... & Eshkind, L. (2017). Inducible knockdown of procollagen I protects mice from liver fibrosis and leads to dysregulated matrix genes and attenuated inflammation. Matrix Biology. https://doi.org/10.1016/j.matbio.2017.11.002.

  • Marttila, S. (2017). Establishment and characterisation of new human induced pluripotent stem cell lines and cardiomyocyte differentiation: a comparative view. Master’s Thesis, University of Tampere, May 2017.

  • Honda, A., Kawano, Y., Izu, H., Choijookhuu, N., Honsho, K., Nakamura, T., ... & Sankai, T. (2017). Discrimination of stem cell status after subjecting cynomolgus monkey pluripotent stem cells to naive conversion. Scientific reports, 7, 45285.

  • Friedel, T., Jung-Klawitter, S., Sebe, A., Schenk, F., Modlich, U., Ivics, Z., ... & Schneider, I. C. (2016). CD30 Receptor-Targeted Lentiviral Vectors for Human Induced Pluripotent Stem Cell-Specific Gene Modification. Stem cells and development, 25(9), 729-739.

  • Ludwig, M., Kitzenberg, D., & Chick, W. S. (2015). Forward Genetic Approach to Uncover Stress Resistance Genes in Mice—A High-throughput Screen in ES Cells. Journal of visualized experiments: JoVE, (105).

  • Neri, T., Muggeo, S., Paulis, M., Caldana, M. E., Crisafulli, L., Strina, D., ... & Scanziani, E. (2015). Targeted gene correction in osteopetrotic-induced pluripotent stem cells for the generation of functional Osteoclasts. Stem cell reports, 5(4), 558-568.

  • Kraus, P., Sivakamasundari, V., Xing, X., & Lufkin, T. (2014). Generating mouse lines for lineage tracing and knockout studies. In Mouse Genetics(pp. 37-62). Humana Press, New York, NY.

  • Zhu, F., Gamboa, M., Farruggio, A. P., Hippenmeyer, S., Tasic, B., Schüle, B., ... & Calos, M. P. (2013). DICE, an efficient system for iterative genomic editing in human pluripotent stem cells. Nucleic acids research, 42(5), e34-e34.

  • Ivics, Z., Izsvák, Z., Chapman, K. M., & Hamra, F. K. (2011). Sleeping Beauty transposon mutagenesis of the rat genome in spermatogonial stem cells. Methods, 53(4), 356-365.

  • Ivics, Z., Izsvák, Z., Medrano, G., Chapman, K. M., & Hamra, F. K. (2011). Sleeping Beauty transposon mutagenesis in rat spermatogonial stem cells. Nature protocols, 6(10), 1521.

  • Pan, Y. (2011). Culturing of C57BL/6 Mouse Embryonic Stem (ES) Cell Line. Bio-protocol Bio101: e142. DOI: 10.21769/BioProtoc.142.

  • Chapman, K. M., Saidley-Alsaadi, D., Syvyk, A. E., Shirley, J. R., Thompson, L. M., & Hamra, F. K. (2011). Rat spermatogonial stem cell-mediated gene transfer. In Advanced Protocols for Animal Transgenesis (pp. 237-266). Springer, Berlin, Heidelberg.

Neo-resistant MEF Feeder Cells

SNL 76/7 (STO Cell Line)

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