• Neural Stem Cell Differentiation

Neural Stem Cell Differentiation

We continually strive to improve and expand our stem cell technology platform, so researchers can leverage our expertise by using our cost-effective and reliable Stem Cell Differentiation Services for studying developmental biology, disease mechanisms, and therapeutic targets.

  • Differentiate your patient-derived iPSCs into self-renewing, multipotent NSCs and further to neurons and glial cells.
  • High differentiation efficiency and cell purity: > 95% cells express key NSC markers
  • Low passage number for robust cell culture
  • Cells fully characterized for NSC biomarkers

We also offer upstream services to generate iPSCs from your patient-derived fibroblasts/PBMCs and downstream cell-based assays services to analyze your differentiated cells or for drug screening applications.

Neural Stem Cell Differentiation Categories

Astrocytes
Differentiation
Service

Custom service to differentiate your iPSCs into NSCs and astrocytes progenitors, along with optimized protocols for culturing mature astrocytes.

Astrocytes
Differentiation
Service

Motor/Cortical
Neuron
Differentiation

Differentiate your iPSCs to NSCs further into motor/ cortical neurons. Differentiate iPSCs from various sources into cortical neurons.

Motor/Cortical
Neuron
Differentiation

Dopaminergic Neuronal
Cell Line

Customized service to differentiate your iPSCs into NSCs and further into midbrain dopaminergic (DA) neuronal precursors.

Dopaminergic Neuronal
Cell Line

Oligodendrocytes
Differentiation
Service

Applied StemCell provides a fully customized service to differentiate your iPSCs into NScs and further into oligodendrocytes precursors.

Oligodendrocytes
Differentiation
Service

Products and Services
Application Notes

 iPSC Differentiation into Neural Stem Cells (NSC)

iPSC Differentiation to Neutral Stem Cells Figure 1 - Neutral Stem Cell Differentiation

Figure 1. iPSCs can be differentiated to NSC in feeder-free condition with high efficiency using Applied StemCell's proprietary neural induction protocol.

iPSC Differentiation to Neutral Stem Cells Figure 2 - Neutral Stem Cell Differentiation

Figure 2. Differentiated NSCs retain neural stem cell phenotype even after free-thaw and passage under feeder-free condtions.

Technical Details

Induced Pluripotent Stem Cells (iPSCs) are exceedingly popular, like embryonic stem cells (ESCs), because their pluripotency allows them to differentiate into all three germ layers with the potential to differentiate into all cell types within the body, but unlike ESCs, they are not encumbered with the ethical dispute associated with the sourcing of ESCs. The true potential of stem cell technology lies in the directed terminal differentiation of iPSCs into specific somatic cells.

The process of iPSC differentiation to neurons and neuronal cells is of special importance for neurobiology and related disorders, considering the dearth of clinically relevant in vitro models available for research, drug screening and development, as well as the lack of therapy to reverse neuronal damage.

Benefits and advantages of iPSC differentiation to neurons and glial cells:

  • Provides genetic and physiologically relevant in vitro models to study neural development and associated disorders: congenital disorders, neurodegenerative disorders and brain tumors.
  • Generate a valuable model for identifying new targets for neuro-regeneration as opposed to treatments limiting to symptomatic relief or delaying disease progression.
  • Allows for future adaptation of technology for regenerative medicine and cell therapy in humans for the treatment of Parkinson’s disease, Lou-Gehrig disease (ALS), Huntington’s disease, and spinal cord injury among other diseases.
  • Differentiation of genome engineered in iPSCs (mutation introduction or correction) to neurons, offers an isogenic source of control-disease cell lines for basic research, drug development, hard-to-model neurological disorders, and potentially for gene therapy.  

Shematic iPSC image - Neutral Stem Cell Differentiation- Schematic iPSC

Neural Stem Cells Differentiation Service

Service Time Deliverables
1. Recovery, Expansion and Validation of iPSCs/ESCs  2-3 weeks

Biweekly updates throughout service

2. Neural Stem Cells Differentiation  (2-6 X10^6 Cells)  2-4 weeks

 

3. Characterization of Differentiated Cells by ICC (per marker)  1 weeks

 

Service Details:

  • For differentiation of iPSCs into neural stem cells, you will need to provide ~1-2 x 10^6 cells

Standard Deliverables:

  •  2-6 X10^6 /line, cryopreserved.
  •  Neural stem cells maintenance medium: 250ml
  •  Reports: NSC marker staining (i.e. SOX1, Pax6) and mycoplasma testing

iPSC Differentiation to Blood Lineage Cells:

Ask us about directed differentiation of your iPSCs into hematopoietic stem cells (HSCs) and blood cells. Avoid sourcing problems associated with HSC procurement, and have a consistent and continuous source of mature human blood cells that are otherwise hard to obtain and maintain. 

Applications: hematopoiesis, cancer immunotherapy, autoimmune diseases/ transplant graft rejection, and drug screening.

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!