Immune Checkpoint Mouse Models

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Applied StemCell’s off-shelf mouse catalog offers >100 humanized immune checkpoint mouse models, with single, double, and triple humanized gene models.

  • Knock-in of humanized counterparts of immune checkpoint gene into the endogenous locus.
  • Proprietary human transgene knock-in strategy to enable screening of many different antibodies and immuno-therapy compounds.
  • In vivo and in vitro validated for physiological expression of human version of the genes (in-house and through collaborators).
  • Fully functional murine immune system  
  • The humanized immune checkpoint mice have been generated in the most widely used C57BL/6J background used in immunology and oncology studies.
  • Ideal for immunology and antibody/ immuno-oncology therapy efficacy studies and drug discovery research.
Products and Services
Application Notes
Single Humanized
Immune-checkpoint Mice
 
 Double Humanized
Immune-checkpoint Mice
Triple Humanized
Immune-checkpoint Mice
 
CTLA4 CD81 PD-1 & GITR PD-1 & PD-L1 & OX40
CTLA4 CEACAM1  PD-L1 & GITR PD-1 & PD-L1 & LAG3
PD-1 CSF1  LAG3 & CTLA4 PD-1 & PD-L1 & PDCD1LG2
PD-L1 CSF2  OX40 & CTLA4 PD-1 & PD-L1 & IDO1
PD-L1 CSF3  PD-1 & CTLA4 PD-1 & TIGIT & TIM3
GITR CXCR2  PD-1 & LAG3 SIRPA & CD47 & PD-1
OX40 DPP4  PD-L1 & CTLA4  
OX40L ENPP1  LAG3 & TIM3  
VISTA FcRn  CTLA4 & ICOS  
CD3E GITR  PD-1 & CD27  
BTLA HGF PD-1 & CD47   
CD276 ICOS  PD-1 & NT5E
CD27 ICOS  PD-L1 & CD27  
CD38 ICOSL  PD-L1 & CD47  
CSF1R IL17F  TIM3 & CEACAM1  
IL6 IL17RA  ICOS & ICOSL  
Nt5e IL1B  PD-1 & TIGIT   
TNFSF15 IL23A  PD-L1 & TIGIT   
VISTA IL2  CD19 & CD3E   
4-1BBL IL2RA  CTLA4 & 4-1BB  
CD4 IL3  IL3 & CSF2   
CD80 IL3  IL6 & IL6R   
CD86 IL4RA  PD-1 & 4-1BB  
IDO1 II5  PD-1 & CD3e   
IL17A IL5RA  PD-1 & CD40  
IL6R IL7  PD-1 & OX40  
OX40 IL9  PD-1 & PD-L1  
PSGL-1 KDR PD-1 & SEMA4D   
TNFRSF25 KLRK1  PD-1 & SIRPA   
VTCN1 LAG3  PD-1 & TIM3  
4-1BB  PCSK9 PD-1 & TLR9   
APOE2 PDCD1LG2  PD-L1 & 4-1BB   
APOE3 PDCD1LG2  PD-L1 & CD40   
APOE4 PVR  PD-L1 & LAG3   
BTLA  SEMA4D  PD-L1 & OX40   
CCR2 SIGLEC15  PD-L1 & SEMA4D  
CCR4 SIRPA  PD-L1 & TIM3   
CCR8 SIRPA  SIRPA & CD47   
CD160 SLAMF7  TLR9 & OX40   
CD19 TIGIT    
CD27 TIGIT     
CD28 TIM3     
CD30 TLR7     
CD36 TLR8    
CD38 TLR9    
CD40 TMEM173    
CD47 TNFRSF1B    

Validation Data for Humanized PD-L1 Mouse

Strain Name: C57BL/6J-Cd274em1(hPD-L1)/Asc        Strain Background: C57BL/6J

Programmed cell death ligand-1 (PD-L1) is a critical immune checkpoint molecule targeted for immunotherapies. This protein, also known as the cluster of differentiation 274 (CD274) or B7 homolog 1 (B7H1), is a transmembrane protein encoded by the CD274 gene and is the ligand for the PD-1 cell surface receptor. The binding of PD-L1 to PD-1 expressed on activated T cells transmits an inhibitory signal that inactivates cytotoxic T cells. This inhibitory mechanism is leveraged by tumor cells which overexpress PD-L1 and thereby inhibit the function of tumor-infiltrating T cells in order to escape immune surveillance. The humanized PD-L1 mouse model enables better translational results to test the efficacy of anti-PD-L1 immunotherapies.

Construction Strategy

appnote-animalmodel-mouserepository-humanicp-pd-l1-1

Figure 1. Generation strategy of humanized PD-L1 mice. The full-length protein coding sequence for human PD-L1 was placed immediately downstream of the start codon of the mouse endogenous Pd-l1 gene, followed by a poly(A) site, so that the expression of endogenous Pd-l1 in the mouse was replaced by the expression of fully humanized PD-L1 protein. This mouse model was generated in the C57BL/6J genetic background.

appnote-animalmodel-mouserepository-humanicp-pd-l1-2

Figure 2. hPD-L1 expression in spleen T cells after stimulation. Flow cytometry analysis of spleen lymphocytes collected from homozygous humanized PD-L1 mice and wild-type C57BL/6J mice. The results showed that the expression of human PD-L1 can be detected in both T cells and B cells collected from the spleen of homozygous humanized PD-L1 mice (completed in collaboration with partners).

appnote-animalmodel-mouserepository-humanicp-pd-l1-1

Figure 3. In vivo validation of anti-tumor efficacy in a MC38 tumor-bearing model of humanized PD-L1 mice. Mouse colon cancer cells MC38 engineered to express human PD-L1 were implanted subcutaneously into homozygous, humanized PD-L1 mice. The mice were randomly assigned into two groups when the tumor volume reached 100 mm3, with one group receiving human IgG as a control and the other receiving a human-specific, PD-L1 antibody (n=5). The human PD-L1 blocking antibody significantly inhibited tumor growth in the homozygous humanized mice (Left) without affecting overall body weight (Right), suggesting that the humanized PD-L1 mice represent an ideal model for evaluating the efficacy of therapeutic antibodies targeting human PD-L1. (TGI: tumor growth inhibition; 67%; p < 0.001), demonstrating that the humanized PD-L1 mice are a good in vivo model for validating the efficacy of antibodies targeting human PD-L1.

Technical Details

Immunotherapy has revolutionized the treatment for cancer by boosting the patient’s own immune system to attack cancer cells. This has been very efficacious in treating many different types of cancer either as a standalone treatment option or in combination with other types of cancer treatment and can be largely attributed to immune checkpoint blockade.

The immune response to an antigen is regulated by a balance between stimulatory and inhibitory signals through a signal transduction pathway of receptors and ligands called Immune checkpoints. Tumor cells hijack these checkpoint pathways by either overexpressing inhibitory immune checkpoints or the suppression of stimulatory immune regulators. These immune checkpoints are the target of a rapidly growing area of immuno-oncology which aims to and train the immune system to recognize and eliminate cancerous cells.

To support the development of these drugs through the clinical stages, preclinical animal models that offer efficient translation of evaluation studies are needed in order to assess the efficacy and safety of these novel therapies. Mouse model are the most commonly and widely accepted in vivo models for preclinical studies. However, the differences in the humans and mouse immune systems with only a 60% homology results in discrepancy in drug performance between preclinical and clinical studies. A preclinical model that mimics the human immune system and cancer development could provide the necessary tumor environment to evaluate the efficacy of these drugs.

To the bridge the gap in bench-to-bedside translation, Applied StemCell has developed 100+ off-shelf, mouse models with knock-in of the human ortholog of immune checkpoint and immunomodulatory genes to replace the entire or a part of the endogenous mouse gene. These humanized knock-in immune checkpoint mouse models offer an ideal platform for developing tumor mouse models for basic research and drug testing.

  • Single, double, triple humanized gene knock-in to enable study of combination immuno-oncology therapies
  • Knock-in of humanized counterparts of immune checkpoint gene into the endogenous locus.
  • Proprietary knock-in strategies in the widely used C57BL/6J background to utilize the complexities of the murine immune system along with the humanized gene expression.
  • Can be combined with CDX and PDX tumor engrafting to develop clinically relevant tumor models.
  • In vivo and in vitro validated for physiological expression of human version of the genes (in-house and through collaborators).
  • Fully functional murine immune system  

These animal models have been validated using tumor engrafts and standard-of-care immunotherapy drugs, and have been successfully used to test the efficacy of novel immunotherapy candidate drugs in early-stage screening for clients before they need expensive GLP studies.

These humanized immune system mouse models are ideal for immunotherapy efficacy studies and drug discovery research for cancer and other immune diseases.

Don’t see a particular mouse model in our repository? We can custom engineer humanized gene knock-in mouse models using our 11+ years of expertise in genetically engineered mouse model (GEMM) generation using CRISPR/Cas9.


Why Choose Applied StemCell?

  • 11+ years of expertise in mouse model genetic engineering
  • Most up-to-date CRISPR protocols for efficiency and affordability
  • Germline transmission included
  • AAALAC-accreditation and SPF facilities
  • We ship globally
  • We also offer downstream validation of your models and drug screening services available
Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!