Animal Models

Mouse Models

• PrimeCKO™ Conditional Knockout Mouse Models

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  Applied StemCell can generate a custom conditional knockout mouse model specific to your needs using CRISPR/Cas9 or other suitable gene editing techniques to insert the 5’ and 3’ flanking LoxP sequences into the endogenous locus of your gene of interest. These conditional knockout mice can be bred with commercially available Cre expressing mice or custom engineered Cre expressing mice to generate your mouse model with precise control over where and when your gene is knocked out.

  • Fast turnaround in as little as 6 months

  • Highly efficient CRISPR protocols and validated gRNA for precise modifications

  • AAALAC-accredited animal facility in the USA

  • Animal IP belongs to customers

  • Custom Cre- mouse generation available

• Knockout, Knock-in, Point Mutation Mouse Models

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  Applied StemCell utilizes its in-licensed CRISPR/Cas9 technology to generate mouse models with precise gene modifications such as knockout, knock-in and point mutation models for a variety of research and preclinical applications.  

  Learn more about our CRISPR Mouse Model Generation Service.

  • Most up-to-date CRISPR design strategies and protocols

  • 100% target-site cutting efficiency using validated gRNA

  • >99% success rate in generating final mouse model

  • AAALAC-accredited animal facility for engineering mouse models

  • Wide variety of genome modifications available

  • F1 breeding to confirm germline transmission

  • Animal IP belongs to researchers

  • Electrophysiology and behavioral assessments for your custom mouse models

• Site-Specific TARGATT™ Knock-in Mouse Models

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  Applied StemCell Inc.’s proprietary TARGATT™ technology uses PhiC31 integrase to insert any gene of interest into a docking site that has been pre-engineered into an intergenic and transcriptionally active genomic locus.

  Advantages of TARGATT™ Technology for Knock-in Mouse Models:

  • Safe harbor locus “large fragment knock-in” (up to 22 kb)

  • High efficiency insertion (up to 40%) 

  • Single copy in an active locus

  Learn more about

  • TARGATT™ Site Specific Knock-in Mouse Model Generation Service

  • Do-It-Yourself! Mouse Model Generation Products

  We can also generate large transgene knock-in mouse models (up to 6 kb) using our highly optimized CRISPR/Cas9 technology, where a gene of interest can be inserted at a specific endogenous locus of choice.

  Learn more about CRISPR/ Cas9 Mouse Model Generation Service

Rat Models

• PrimeCKO™ Conditional Knockout Rat Models

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  Applied StemCell can generate a custom conditional knockout rat model using CRISPR/Cas9 or other suitable gene editing techniques to insert the 5’ and 3’ flanking LoxP sequences into the endogenous locus of your gene of interest. These conditional knockout rats can be bred with commercially available Cre expressing rats or custom engineered Cre expressing rats to generate your rat model with precise control over where and when your gene is knocked out.

  Learn more about our CRISPR Conditional Knockout Rat Model Generation

  ASC offers Bacterial Artificial Chromosomes (BACs) to generate rat models. BACs are ultra-low copy vectors that hold up to 300 kb of genomic fragments.

  Learn more about our BAC Conditional Knockout Rat Model Generation

• Site-Specific TARGATT™ Knock-in Rat Models

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  Applied StemCell Inc.’s proprietary TARGATT™ technology uses PhiC31 integrase to insert any gene of interest into a docking site that has been pre-engineered into an intergenic and transcriptionally active genomic locus.

  Site-Specific Transgenic TARGATT™ Rat Model:

  • High efficiency gene integration (up to 40%)

  • Single copy, large DNA fragment knock-in (up to 22 kb)

  • Site-specific gene insertion into a preselected, safe harbor locus

  • Unidirectional integration

  • Direct injection of the DNA into rat zygotes

  • Non-immunogenic TARGATT™ plasmid

  • Works independent of cell division

  • Sprague-Dawley rats

  • Founder rats in 4-6 months

  • Advantageous in vivo model for researchers seeking better representation of human diseases

• Knockout, Knock-in, Point Mutation Rat Models

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  Applied StemCell (ASC) leverages its long-standing expertise in CRISPR/Cas9 technology, as well as animal model engineering to generate rat models with precise gene modifications for a variety of research and preclinical applications.

  Key Features:

  • Up-to-date CRISPR design strategies

  • 100% target-site cutting efficiency using validated gRNA

  • Highly successful at generating final rat model

  • ISO:9001 certified facility for manufacturing CRISPR reagents

  • AAALAC-accredited animal facility in the USA

  • Wide variety of genome modifications available

  • F1 breeding to confirm germline transmission

  • Animal IP belongs to researchers

  • Electrophysiology and behavioral assessments for your rat models

   Learn about our CRISPR Rat Model Generation Service

Custom In Vivo Assay Services