Animal Models
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Mouse Models
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PrimeCKO™ Conditional Knockout Mouse Models
Applied StemCell can generate a custom conditional knockout mouse model specific to your needs using CRISPR/Cas9 or other suitable gene editing techniques to insert the 5’ and 3’ flanking LoxP sequences into the endogenous locus of your gene of interest. These conditional knockout mice can be bred with thousands of commercially available Cre-deleter mice or custom engineered Cre-expressing mice to generate your mouse model with precise control over where and when your gene is knocked out.
- Fast (as little as 6 months) and affordable (very low price)
- Highly efficient CRISPR protocols and validated gRNA for precise modifications
- Animal IP belongs to customers
- Custom Cre- mouse generation available
- F1 breeding for germline transmission
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Knockout, Knock-in, Point Mutation Mouse Models
Applied StemCell utilizes its in-licensed CRISPR/Cas9 technology to generate mouse models with precise gene modifications such as knockout, knock-in and point mutation models for a variety of research and preclinical applications.
Learn more about our CRISPR Mouse Model Generation Service.
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Most up-to-date CRISPR design strategies and protocols
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100% target-site cutting efficiency using validated gRNA
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>99% success rate in generating final mouse model
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Wide variety of genome modifications available
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F1 breeding to confirm germline transmission
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Animal IP belongs to researchers
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Electrophysiology and behavioral assessments for your custom mouse models
Summer Sale! Custom Knockout Mouse Model Generation $10K USD
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Site-Specific TARGATT™ Knock-in Mouse Models
Applied StemCell Inc.’s proprietary TARGATT™ technology uses PhiC31 integrase to insert any gene of interest into a docking site that has been pre-engineered into an intergenic and transcriptionally active genomic locus.
ASC’s proprietary TARGATT™ knockin technology enables highly efficient and site-specific gene knockin mouse model generation. This technology uses serine integrase, PhiC31 (jC31) to insert any gene of interest into a preselected intergenic and transcriptionally active genomic locus that has been engineered with a docking site for stable, site-specific transgene integration.
- High efficiency insertion (up to 65%)
- Site-specific into a genetically active safe harbor locus
- Large fragment knockin (up to 22 kb)
- Single copy in an active locus: avoid gene silencing and genomic instability.
- High level, stable gene expression
- Germline transmitted F1 mice in 5-8 months
Mouse models: Gene overexpression, conditional expression, humanized gene knockin, gene knockdown, reporter genes
Site-specific TARGATT™ Mouse Models -
Knockout Mouse Model Generation
We can generate a gene knockout mouse model for inactivating genes either by frameshift mutation or by fragment excision, using CRISPR/Cas9. Gene knockout or disruption in mouse models provide an in vivo environment to study the function or role of the gene of interest. Applied StemCell has optimized and most-up-to date gene targeting strategies to generate the knockout mouse model of your choice. We can generate constitutive knockout mice or for a more advance study of the gene function, conditional/ inducible knockout models in as little as 5 months (F1 germline transmitted).
Knockout Mouse Model Generation Services:
- Gene Deletion
- Conventional - Quick Knockout
- Conditional Knockout - Tissue Specific or Inducible
- Point Mutation - Loss of Function
Summer Sale! Custom Knockout Mouse Model Generation $10K USD
- Gene Deletion
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Knockin Mouse Model Generation
Applied StemCell offers custom knockin mouse model generation service using two complementary technologies, CRISPR/Cas9 and TARGATT™ site-specific integration technologies that vastly expand the portfolio of knockin modifications we can offer gene-specific knockin (up to 6 kb) or safe harbor locus knockin (up to 10kb with CRISPR and up to 22 kb with TARGATT™). We offer several genetic modifications: point mutation, humanized gene knockin/ replacement, reporter mouse models, safe harbor locus models with CRISPR (Rosa26, H11, AAVS1) or TARGATT™ (Rosa26, H11 or a custom locus).
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Mouse Repository
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Humanized ACE2 Mice for COVID-19 Research
Humanized ACE2 Mouse Models Available Now
To aid the global research efforts to find a solution to the COVID-19 pandemic, Applied StemCell has leveraged its site-specific TARGATT™ knockin technology to engineer two fully humanized ACE2 (hACE2) mouse models with lung epithelium-specific expression driven by the K18 promoter. These TARGATT™ hACE2 mouse models have the K18-hACE2 transgene integrated into a preselected, well-characterized safe harbor locus (genomic hotspots): mH11 or mRosa26 and might be more representative to model the severity of the SARS-CoV-2 infection. The TARGATT™ hACE2 mouse models are ideal to help understand the severe pathogenesis of COVID-19, and to evaluate the efficacy of antiviral drugs and vaccines.
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Immune Checkpoint Mouse Models
Worldwide shipping!
Applied StemCell’s off-shelf mouse catalog offers >100 humanized immune checkpoint mouse models, with single, double, and triple humanized gene models.
- Knock-in of humanized counterparts of immune checkpoint gene into the endogenous locus.
- Proprietary human transgene knock-in strategy to enable screening of many different antibodies and immuno-therapy compounds.
- In vivo and in vitro validated for physiological expression of human version of the genes (in-house and through collaborators).
- Fully functional murine immune system
- The humanized immune checkpoint mice have been generated in the most widely used C57BL/6J background used in immunology and oncology studies.
- Ideal for immunology and antibody/ immuno-oncology therapy efficacy studies and drug discovery research.
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Antibody Efficacy Testing Service
Leverage ASCs cost-effective evaluation of in vivo efficacy and fast timelines to screen your early-stage lead antibodies using our antibody efficacy testing platform:
Workflow includes:
- Project Proposal: details on the test antibody (and positive and negative control), project design, scope of the project
- Receive antibody(ies) from client
- Tumor cell implantation and development in humanized immune checkpoint mouse models (mean tumor size reaches 100-150 mm^3)
- Administration of antibodies to humanized immune checkpoint mouse tumor models
- In vivo evaluation of antibody efficacy: tumor size and body weight, twice a week for 3-4 weeks
- Final report with data and figures
- Report will include comprehensive information about experimental/ project outline, project schedule, survey notes, raw data for efficacy parameters measured/ endpoints, calculated/ integrated results, raw and integrated figures (graphs and other figures corresponding to endpoint measurements).
* Endpoint: Tumor burden should not exceed 10% of the animal’s bodyweight. The study will be terminated with euthanizing all animals when the mean tumor volume reaches about 3000 mm^3.
Optional post-mortem tests include: Blood RT/Biochemical analysis; FACS analysis on PBMC (including CBA); serum/ plasma preparation and then ELISA, MSD or WB analysis, qPCR,WB, IHC, IF, TILs analysis
Timeline: 2-3 months after receipt of antibodies
LEARN MORE
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Cre Mouse Models
Inquire!
The Cre-Lox system is a powerful gene manipulation tool that enables controlled gene expression in a specific tissue or by induction in mouse models. Such controlled/ conditional gene expression or ablation enables deeper study of gene function without problems such as artificial phenotype(s) and embryonic lethality.
Applied StemCell offers several ready-to-use, mouse models expressing Cre recombinase under the control of various tissue-specific and/or inducible promoters as well as Cre-reporter mice:
- Well-characterized Cre driver mouse lines
- Generate temporal and/or spatial control of gene expression mouse models
- Breed with your loxP floxed mice or we can generate a custom floxed mouse for you using our PrimeCKo™ Conditional Mouse Model Generation Service; in your facility or ours
Available Cre Mouse Lines
Cre-Mouse Lines Alb-(CreERT2) Cdkn2a-(Luc-tdTomato-CreERT2) Isl1-(CreERT2) Pdx1-(Avi-CreERT2) Map2-(CreERT2) Lyz2-(CreERT2-EGFP) Dppa3-(Cre) Piezo1-(CreERT2) Cyp2e1-(CreERT2) Tyr-(CreERT2) Il17a-(CreERT2) Slc17a6-(CreERT2) Myh6-(Cre) Amhr2-(Cre) Epcam-(CreERT2) Cd68-(CreERT2) H11-(ddCre) Krt5-(2A-CreERT2) Kit-CreERT2 Ctsk-(2A-Cre) Nphs2-CreERT2 Krt14-(CreERT2) Aplnr-CreXER2 Adgre1-(2A-CreERT2) Foxl1-CreERT2 Ncr1-(2A-iCre Hey2-CreERT2 Ly6a-(2A-iCre) Cdh5-(rtTA-tetO-Cre) Csf1r-(IRES-iCre Col1a1-e(TetO-GFPCre-M2rtTA) Sst-(IRES-Cre) Drd1-(2A-CreERT2-WPRE-pA) Afp-CreERT2 Cd2-(2A-CreERT2-WPRE-pA) Amhr2-Cre Lyz2-(2A-CreERT2-WPRE-pA) Lrat-(2A-Cre) Sftpc-(IRES-CreERT2) Ucp1-(CreERT2) Zbtb46-(2A-CreERT2-WPRE-pA) Epx-(iCre) Tie1-(2A-Cre) Ucp1-(Cre) Col2a1-(2A-CreERT2) Nkx3-1-(CreERT2) Tert-(CreERT2) -
Cytokine Mouse Models
Inquire!
Cytokines are cell-to-cell signaling proteins that play a central role in immune development and responses, and its dysregulation is associated with a several diseases such as autoimmune and inflammatory disorders, chronic infections and even cancer. Applied StemCell offers research-ready, mouse models expressing humanized cytokine genes for studying the underlying mechanisms of immune response and inflammation as well as for immunotherapy and oncology efficacy studies and drug screening.
- Knock-in of humanized counterparts of cytokine gene(s) into the endogenous locus.
- In vivo and in vitro validated for physiological expression of human version of the genes (in-house and through collaborators).
- Mouse models generated in the widely used C57BL/6J background.
Cytokine Mouse Models
Model Name Genes Gene Synonyms Human Orthologs IL4RA-HU Il4ra Il4r; CD124 IL4R IL17A-HU Il17a Il17 IL17A IL6R-HU Il6ra CD126, IL-6R IL-6R IL6-HU Il6 Il-6 IL6 IL9-HU Il9 P40, Il-9 IL9 IL23A-HU Il23a p19, IL-23 IL23A Il5-HU Il5 Il-5 IL5 IL17F-HU Il17f C87042; IL-17F IL17F IL3-HU Il3 BPA; PSF; HCGF; Il-3; MCGF; Csfmu IL3 IL6&IL6R-HU Il6; Il6ra Il-6; CD126, IL-6R IL6; IL-6R IL7-HU Il7 Il-7; hlb368; A630026I06Rik IL7 IL3-HU Il3 BPA, Csfmu, HCGF, Il-3, MCGF, PSF IL3 IL1B-HU Il1b Il-1b; IL-1beta IL1B IL2-HU Il2 IL-2 IL2 IL17RA-HU Il17ra Il17r; Cdw217; VDw217; AW538159 IL17RA IL5RA-HU Il5ra Il5r; CD125; CDw125 IL5RA IL2RA-HU Il2ra CD25; Il2r; Ly-43 IL2RA IL3&CSF2-HU Il3; Csf2 BPA, Csfmu, HCGF, Il-3, MCGF, PSF; CSF; Csfgm; GMCSF; Gm-CSf; MGI-IGM IL3; CSF2 -
Other Gene Edited Mouse Models (KO, CKO)
Inquire!
With 11+ years of experience in genetically engineering mouse models (GEMM), Applied StemCell offers a wide-range of ready-to-use, constitutive knockout (KO) and conditional knockout (CKO) mouse models targeting various genes, with some very unique models available only here. Our GEMM catalog also includes reporter/ tag knock-in, inducible expression mouse models and other tool mice, to expand the scope of your research and screening applications.
- Genetically validated models
- Majority of the lines are in the C57BL/6 background
- Facilities operated under the strict AAALAC, SPF and IACUC guidelines
- Can be shipped worldwide
- Valuable research tools for genetics research, disease modeling and drug screening
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Immunodeficient Mouse Models
Inquire!
Applied StemCell provides next-generation immunodeficient mouse models such as our proprietary M-NSG (NOD-scid: non-obese diabetic severe combined immune deficiency, gamma), RAG1, RAG2 knockout (KO) models and more. Our M-NSG mice lack mature T cells, B cells, and functional NK cells.
- Highest degree of immunodeficiency
- Long-lifespan
- Excellent tumor xenograft (PDX & CDX) models with minimal immune rejection
- Ideal recipient for human immune reconstitution (humanized mice). Ex. hPBMC/ hHSC engraftment
- Applications include immuno-oncology, immunodeficiency, inflammation, transplantation and immune mechanism
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Rat Models
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PrimeCKO™ Conditional Knockout Rat Models
Applied StemCell can generate a custom conditional knockout rat model using CRISPR/Cas9 or other suitable gene editing techniques to insert the 5’ and 3’ flanking LoxP sequences into the endogenous locus of your gene of interest. These conditional knockout rats can be bred with commercially available Cre expressing rats or custom engineered Cre expressing rats to generate your rat model with precise control over where and when your gene is knocked out.
Learn more about our CRISPR Conditional Knockout Rat Model Generation
ASC offers Bacterial Artificial Chromosomes (BACs) to generate rat models. BACs are ultra-low copy vectors that hold up to 300 kb of genomic fragments.
Learn more about our BAC Conditional Knockout Rat Model Generation
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Site-Specific TARGATT™ Knock-in Rat Models
Applied StemCell Inc.’s proprietary TARGATT™ technology uses PhiC31 integrase to insert any gene of interest into a docking site that has been pre-engineered into an intergenic and transcriptionally active genomic locus.
Site-Specific Transgenic TARGATT™ Rat Model:
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High efficiency gene integration (up to 40%)
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Single copy, large DNA fragment knock-in (up to 22 kb)
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Site-specific gene insertion into a preselected, safe harbor locus
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Unidirectional integration
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Direct injection of the DNA into rat zygotes
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Non-immunogenic TARGATT™ plasmid
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Works independent of cell division
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Sprague-Dawley rats
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Founder rats in 4-6 months
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Advantageous in vivo model for researchers seeking better representation of human diseases
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Knockout, Knock-in, Point Mutation Rat Models
Applied StemCell (ASC) leverages its long-standing expertise in CRISPR/Cas9 technology, as well as animal model engineering to generate rat models with precise gene modifications for a variety of research and preclinical applications.
Key Features:
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Up-to-date CRISPR design strategies
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100% target-site cutting efficiency using validated gRNA
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Highly successful at generating final rat model
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ISO:9001 certified facility for manufacturing CRISPR reagents
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Wide variety of genome modifications available
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F1 breeding to confirm germline transmission
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Animal IP belongs to researchers
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Electrophysiology and behavioral assessments for your rat models
Learn about our CRISPR Rat Model Generation Service
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Cre Rat Models
These transgenic Cre rat models have been developed using either ASC's proprietary integrase-based TARGATT™ System or CRISPR/Cas9 genome editing technology. These Cre rat lines can enable the development of physiologically relevant human cardiovascular, neurological diseases and other disease specific models.
These products were supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R44GM108071. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Applied StemCell, Inc. (ASC) announces that as of July 1, 2020, customers can order the Cre rat lines for research and industrial applications, through our partnering with the Rat Resource & Research Center (RRRC). For more questions regarding the Cre rat models, please visit the RRRC website at www.rrrc.us or contact the RRRC customer service email at rrrc@missouri.edu.
These strains will be available as:
RRRC# Strain Name
868 Wnt1-CreERT2
869 PDGFb-CreERT2
870 MOR23-CreERT2
872 HB9-CreERT2
873 Drd1a-CreERT2
874 Gad67-CreERT2
876 GFAP-CreERT2
877 Tie2-CreERT2
878 SMHC-CreERT2
879 CAG-LSL-GFP-LacZ
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Knockout Rat Model Generation
Applied StemCell offers Quick Knockout Mouse Model Generation for engineering constitutive gene deletion or mutation (frameshift) to render a gene of interest non-functional. Applied StemCell offers high quality custom genetically engineered mouse model (GEMM) generation service operated out of vivarium in AAALAC-accredited facilities with strict IACUC oversight. Our knockout mouse model protocols are very optimized and efficient to offer a >97% success rate. Order projects with us a stress-free research animal model generation that will suit your basic research and drug development needs.
Mouse Models Categories -
Knockin Rat Model Generation
Rat models are very important models for specialized research where mouse models are not quite representative of the human physiology or behavior. Like mouse models, targeted genetic engineering in rat models has been revolutionized with CRISPR/Cas9 and other technologies (TARGATT™, TALENs and ZFNs). Applied StemCell offers knockin rat model generation using two technologies: CRISPR/Cas9 and TARGATT™ to enable the engineering a wide range of knockin modifications.
Mouse Models Categories
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Custom In Vivo Assay Services
