Animal Models

  • Mouse Models

    • PrimeCKO™ Conditional Knockout Mouse Models


      Applied StemCell can generate a custom conditional knockout mouse model specific to your needs using CRISPR/Cas9 or other suitable gene editing techniques to insert the 5’ and 3’ flanking LoxP sequences into the endogenous locus of your gene of interest. These conditional knockout mice can be bred with thousands of commercially available Cre-deleter mice or custom engineered Cre-expressing mice to generate your mouse model with precise control over where and when your gene is knocked out.

      • Fast (as little as 6 months) and affordable (very low price)
      • Highly efficient CRISPR protocols and validated gRNA for precise modifications
      • Animal IP belongs to customers
      • Custom Cre- mouse generation available
      • F1 breeding for germline transmission
    • Knockout, Knock-in, Point Mutation Mouse Models


      Applied StemCell utilizes its in-licensed CRISPR/Cas9 technology to generate mouse models with precise gene modifications such as knockout, knock-in and point mutation models for a variety of research and preclinical applications.  

      Learn more about our CRISPR Mouse Model Generation Service.

      • Most up-to-date CRISPR design strategies and protocols

      • 100% target-site cutting efficiency using validated gRNA

      • >99% success rate in generating final mouse model

      • Wide variety of genome modifications available

      • F1 breeding to confirm germline transmission

      • Animal IP belongs to researchers

      • Electrophysiology and behavioral assessments for your custom mouse models

    • Site-Specific TARGATT™ Knock-in Mouse Models


      Applied StemCell Inc.’s proprietary TARGATT™ technology uses PhiC31 integrase to insert any gene of interest into a docking site that has been pre-engineered into an intergenic and transcriptionally active genomic locus.

      ASC’s proprietary TARGATT™ knockin technology enables highly efficient and site-specific gene knockin mouse model generation. This technology uses serine integrase, PhiC31 (jC31) to insert any gene of interest into a preselected intergenic and transcriptionally active genomic locus that has been engineered with a docking site for stable, site-specific transgene integration.

      • High efficiency insertion (up to 65%)
      • Site-specific into a genetically active safe harbor locus
      • Large fragment knockin (up to 22 kb)
      • Single copy in an active locus: avoid gene silencing and genomic instability.
      • High level, stable gene expression
      • Germline transmitted F1 mice in 5-8 months

      Mouse models: Gene overexpression, conditional expression, humanized gene knockin, gene knockdown, reporter genes

      Site-specific TARGATT™ Mouse Models

      Site-Specific Knock-In TARGATT “attP” Mice

      TARGATT™ attP mice available at Applied StemCell, along with associated kits enable you to make site-specific transgenic mouse models in your lab.

      Site-Specific Knock-In TARGATT “attP” Mice

    • Knockout Mouse Model Generation


      We can generate a gene knockout mouse model for inactivating genes either by frameshift mutation or by fragment excision, using CRISPR/Cas9. Gene knockout or disruption in mouse models provide an in vivo environment to study the function or role of the gene of interest. Applied StemCell has optimized and most-up-to date gene targeting strategies to generate the knockout mouse model of your choice. We can generate constitutive knockout mice or for a more advance study of the gene function, conditional/ inducible knockout models in as little as 5 months (F1 germline transmitted).

    • Knockin Mouse Model Generation


      Applied StemCell offers custom knockin mouse model generation service using two complementary technologies, CRISPR/Cas9 and TARGATT™ site-specific integration technologies that vastly expand the portfolio of knockin modifications we can offer gene-specific knockin (up to 6 kb) or safe harbor locus knockin (up to 10kb with CRISPR and up to 22 kb with TARGATT™). We offer several genetic modifications: point mutation, humanized gene knockin/ replacement, reporter mouse models, safe harbor locus models with CRISPR (Rosa26, H11, AAVS1) or TARGATT™ (Rosa26, H11 or a custom locus).

  • Mouse Repository

    • Humanized ACE2 Mice for COVID-19 Research


      Humanized ACE2 Mouse Models Available for Shipping Mid-December 2020! To aid the global research efforts to find a solution to the COVID-19 pandemic, Applied StemCell has leveraged its site-specific TARGATT™ knockin technology to engineer two fully humanized ACE2 (hACE2) mouse models with lung epithelium-specific expression driven by the K18 promoter. These TARGATT™ hACE2 mouse models have the K18-hACE2 transgene integrated into a preselected, well-characterized safe harbor locus (genomic hotspots): mH11 or mRosa26 and might be more representative to model the severity of the SARS-CoV-2 infection. The TARGATT™ hACE2 mouse models are ideal to help understand the severe pathogenesis of COVID-19, and to evaluate the efficacy of antiviral drugs and vaccines.

    • Immune Checkpoint Mouse Models


      Worldwide shipping! 

      Applied StemCell’s off-shelf mouse catalog offers >100 humanized immune checkpoint mouse models, with single, double, and triple humanized gene models.

      • Knock-in of humanized counterparts of immune checkpoint gene into the endogenous locus.
      • Proprietary human transgene knock-in strategy to enable screening of many different antibodies and immuno-therapy compounds.
      • In vivo and in vitro validated for physiological expression of human version of the genes (in-house and through collaborators).
      • Fully functional murine immune system  
      • The humanized immune checkpoint mice have been generated in the most widely used C57BL/6J background used in immunology and oncology studies.
      • Ideal for immunology and antibody/ immuno-oncology therapy efficacy studies and drug discovery research.
    • Cre Mouse Models


      Inquire!

      The Cre-Lox system is a powerful gene manipulation tool that enables controlled gene expression in a specific tissue or by induction in mouse models. Such controlled/ conditional gene expression or ablation enables deeper study of gene function without problems such as artificial phenotype(s) and embryonic lethality.

      Applied StemCell offers several ready-to-use, mouse models expressing Cre recombinase under the control of various tissue-specific and/or inducible promoters as well as Cre-reporter mice:

      • Well-characterized Cre driver mouse lines
      • Generate temporal and/or spatial control of gene expression mouse models
      • Breed with your loxP floxed mice or we can generate a custom floxed mouse for you using our PrimeCKo™ Conditional Mouse Model Generation Service; in your facility or ours
       

      Available Cre Mouse Lines

      Cre-Mouse Lines
      Alb-(CreERT2) Cdkn2a-(Luc-tdTomato-CreERT2)
      Isl1-(CreERT2) Pdx1-(Avi-CreERT2)
      Map2-(CreERT2) Lyz2-(CreERT2-EGFP)
      Dppa3-(Cre) Piezo1-(CreERT2)
      Cyp2e1-(CreERT2) Tyr-(CreERT2)
      Il17a-(CreERT2) Slc17a6-(CreERT2)
      Myh6-(Cre) Amhr2-(Cre)
      Epcam-(CreERT2) Cd68-(CreERT2)
      H11-(ddCre) Krt5-(2A-CreERT2)
      Kit-CreERT2 Ctsk-(2A-Cre)
      Nphs2-CreERT2 Krt14-(CreERT2)
      Aplnr-CreXER2 Adgre1-(2A-CreERT2)
      Foxl1-CreERT2 Ncr1-(2A-iCre
      Hey2-CreERT2 Ly6a-(2A-iCre)
      Cdh5-(rtTA-tetO-Cre) Csf1r-(IRES-iCre
      Col1a1-e(TetO-GFPCre-M2rtTA) Sst-(IRES-Cre)
      Drd1-(2A-CreERT2-WPRE-pA) Afp-CreERT2
      Cd2-(2A-CreERT2-WPRE-pA) Amhr2-Cre
      Lyz2-(2A-CreERT2-WPRE-pA) Lrat-(2A-Cre)
      Sftpc-(IRES-CreERT2) Ucp1-(CreERT2)
      Zbtb46-(2A-CreERT2-WPRE-pA) Epx-(iCre)
      Tie1-(2A-Cre) Ucp1-(Cre)
      Col2a1-(2A-CreERT2)  
      Nkx3-1-(CreERT2)  
      Tert-(CreERT2)  
    • Cytokine Mouse Models


      Inquire!

      Cytokines are cell-to-cell signaling proteins that play a central role in immune development and responses, and its dysregulation is associated with a several diseases such as autoimmune and inflammatory disorders, chronic infections and even cancer. Applied StemCell offers research-ready, mouse models expressing humanized cytokine genes for studying the underlying mechanisms of immune response and inflammation as well as for immunotherapy and oncology efficacy studies and drug screening.

      • Knock-in of humanized counterparts of cytokine gene(s) into the endogenous locus.
      • In vivo and in vitro validated for physiological expression of human version of the genes (in-house and through collaborators).
      • Mouse models generated in the widely used C57BL/6J background.

       

      Cytokine Mouse Models

      Model Name Genes Gene Synonyms Human Orthologs
      IL4RA-HU Il4ra Il4r; CD124 IL4R
      IL17A-HU Il17a Il17 IL17A
      IL6R-HU Il6ra CD126, IL-6R IL-6R
      IL6-HU Il6 Il-6 IL6
      IL9-HU Il9 P40, Il-9 IL9
      IL23A-HU Il23a p19, IL-23 IL23A
      Il5-HU Il5 Il-5 IL5
      IL17F-HU Il17f C87042; IL-17F IL17F
      IL3-HU Il3 BPA; PSF; HCGF; Il-3; MCGF; Csfmu IL3
      IL6&IL6R-HU Il6; Il6ra Il-6; CD126, IL-6R IL6; IL-6R
      IL7-HU Il7 Il-7; hlb368; A630026I06Rik IL7
      IL3-HU Il3 BPA, Csfmu, HCGF, Il-3, MCGF, PSF IL3
      IL1B-HU Il1b Il-1b; IL-1beta IL1B
      IL2-HU Il2 IL-2 IL2
      IL17RA-HU Il17ra Il17r; Cdw217; VDw217; AW538159 IL17RA
      IL5RA-HU Il5ra Il5r; CD125; CDw125 IL5RA
      IL2RA-HU Il2ra CD25; Il2r; Ly-43 IL2RA
      IL3&CSF2-HU Il3; Csf2 BPA, Csfmu, HCGF, Il-3, MCGF, PSF; CSF; Csfgm; GMCSF; Gm-CSf; MGI-IGM IL3; CSF2
    • Other Gene Edited Mouse Models (KO, CKO)


      Inquire!

      With 11+ years of experience in genetically engineering mouse models (GEMM), Applied StemCell offers a wide-range of ready-to-use, constitutive knockout (KO) and conditional knockout (CKO) mouse models targeting various genes, with some very unique models available only here. Our GEMM catalog also includes reporter/ tag knock-in, inducible expression mouse models and other tool mice, to expand the scope of your research and screening applications.

      • Genetically validated models
      • Majority of the lines are in the C57BL/6 background
      • Facilities operated under the strict AAALAC, SPF and IACUC guidelines
      • Can be shipped worldwide
      • Valuable research tools for genetics research, disease modeling and drug screening
    • Immunodeficient Mouse Models


      Inquire!

      Applied StemCell provides next-generation immunodeficient mouse models such as our proprietary M-NSG (NOD-scid: non-obese diabetic severe combined immune deficiency, gamma), RAG1, RAG2 knockout (KO) models and more. Our M-NSG mice lack mature T cells, B cells, and functional NK cells.

      • Highest degree of immunodeficiency
      • Long-lifespan
      • Excellent tumor xenograft (PDX & CDX) models with minimal immune rejection
      • Ideal recipient for human immune reconstitution (humanized mice). Ex. hPBMC/ hHSC engraftment
      • Applications include immuno-oncology, immunodeficiency, inflammation, transplantation and immune mechanism
  • Rat Models

    • PrimeCKO™ Conditional Knockout Rat Models


      Applied StemCell can generate a custom conditional knockout rat model using CRISPR/Cas9 or other suitable gene editing techniques to insert the 5’ and 3’ flanking LoxP sequences into the endogenous locus of your gene of interest. These conditional knockout rats can be bred with commercially available Cre expressing rats or custom engineered Cre expressing rats to generate your rat model with precise control over where and when your gene is knocked out.

      Learn more about our CRISPR Conditional Knockout Rat Model Generation

      ASC offers Bacterial Artificial Chromosomes (BACs) to generate rat models. BACs are ultra-low copy vectors that hold up to 300 kb of genomic fragments.

      Learn more about our BAC Conditional Knockout Rat Model Generation

    • Site-Specific TARGATT™ Knock-in Rat Models


      Applied StemCell Inc.’s proprietary TARGATT™ technology uses PhiC31 integrase to insert any gene of interest into a docking site that has been pre-engineered into an intergenic and transcriptionally active genomic locus.

      Site-Specific Transgenic TARGATT™ Rat Model:

      • High efficiency gene integration (up to 40%)

      • Single copy, large DNA fragment knock-in (up to 22 kb)

      • Site-specific gene insertion into a preselected, safe harbor locus

      • Unidirectional integration

      • Direct injection of the DNA into rat zygotes

      • Non-immunogenic TARGATT™ plasmid

      • Works independent of cell division

      • Sprague-Dawley rats

      • Founder rats in 4-6 months

      • Advantageous in vivo model for researchers seeking better representation of human diseases

    • Knockout, Knock-in, Point Mutation Rat Models


      Applied StemCell (ASC) leverages its long-standing expertise in CRISPR/Cas9 technology, as well as animal model engineering to generate rat models with precise gene modifications for a variety of research and preclinical applications.

      Key Features:

      • Up-to-date CRISPR design strategies

      • 100% target-site cutting efficiency using validated gRNA

      • Highly successful at generating final rat model

      • ISO:9001 certified facility for manufacturing CRISPR reagents

      • Wide variety of genome modifications available

      • F1 breeding to confirm germline transmission

      • Animal IP belongs to researchers

      • Electrophysiology and behavioral assessments for your rat models

       Learn about our CRISPR Rat Model Generation Service

    • Cre Rat Models


      These transgenic Cre rat models have been developed using either ASC's proprietary integrase-based TARGATT™ System or CRISPR/Cas9 genome editing technology. These Cre rat lines can enable the development of physiologically relevant human cardiovascular, neurological diseases and other disease specific models.

      These products were supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R44GM108071. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

      Applied StemCell, Inc. (ASC) announces that as of July 1, 2020, customers can order the Cre rat lines for research and industrial applications, through our partnering with the Rat Resource & Research Center (RRRC). For more questions regarding the Cre rat models, please visit the RRRC website at www.rrrc.us or contact the RRRC customer service email at rrrc@missouri.edu.

      These strains will be available as:

      RRRC#            Strain Name

      868                  Wnt1-CreERT2

      869                  PDGFb-CreERT2

      870                  MOR23-CreERT2

      872                  HB9-CreERT2

      873                  Drd1a-CreERT2

      874                  Gad67-CreERT2

      876                  GFAP-CreERT2

      877                  Tie2-CreERT2

      878                  SMHC-CreERT2

      879                  CAG-LSL-GFP-LacZ

    • Knockout Rat Model Generation


      Applied StemCell offers Quick Knockout Mouse Model Generation for engineering constitutive gene deletion or mutation (frameshift) to render a gene of interest non-functional. Applied StemCell offers high quality custom genetically engineered mouse model (GEMM) generation service operated out of vivarium in AAALAC-accredited facilities with strict IACUC oversight. Our knockout mouse model protocols are very optimized and efficient to offer a >97% success rate. Order projects with us a stress-free research animal model generation that will suit your basic research and drug development needs.

      Mouse Models Categories
    • Knockin Rat Model Generation


      Rat models are very important models for specialized research where mouse models are not quite representative of the human physiology or behavior. Like mouse models, targeted genetic engineering in rat models has been revolutionized with CRISPR/Cas9 and other technologies (TARGATT™, TALENs and ZFNs). Applied StemCell offers knockin rat model generation using two technologies: CRISPR/Cas9 and TARGATT™ to enable the engineering a wide range of knockin modifications.

      Mouse Models Categories
  • Custom In Vivo Assay Services

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!

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