Animal Models

Mouse Models
- PrimeCKO™ Conditional Knockout Mouse Models
  Applied StemCell can generate a custom conditional knockout mouse model specific to your needs using CRISPR/Cas9 or other suitable gene editing techniques to insert the 5’ and 3’ flanking LoxP sequences into the endogenous locus of your gene of interest. These conditional knockout mice can be bred with thousands of commercially available Cre-deleter mice or custom engineered Cre-expressing mice to generate your mouse model with precise control over where and when your gene is knocked out.
  - Fast (as little as 6 months) and affordable (very low price)
  - Highly efficient CRISPR protocols and validated gRNA for precise modifications
  - Animal IP belongs to customers
  - Custom Cre- mouse generation available
  - Founder guaranteed
- Knockout, Knock-in, Point Mutation Mouse Models
  Applied StemCell utilizes its in-licensed CRISPR/Cas9 technology to generate mouse models with precise gene modifications such as knockout, knock-in and point mutation models for a variety of research and preclinical applications.
  
  Learn more about our CRISPR Mouse Model Generation Service.
  - Most up-to-date CRISPR design strategies and protocols
  - 100% target-site cutting efficiency using validated gRNA
  - >99% success rate in generating final mouse model
  - Wide variety of genome modifications available
  - F1 breeding to confirm germline transmission
  - Animal IP belongs to researchers
  - Electrophysiology and behavioral assessments for your custom mouse models
- Site-Specific TARGATT™ Knock-in Mouse Models
  Applied StemCell Inc.’s proprietary TARGATT™ technology uses PhiC31 integrase to insert any gene of interest into a docking site that has been pre-engineered into an intergenic and transcriptionally active genomic locus.
  
  Advantages of TARGATT™ Technology for Knock-in Mouse Models:
  - Safe harbor locus “large fragment knock-in” (up to 22 kb)
  - High efficiency insertion (up to 40%)
  - Single copy in an active locus
  Learn more about
  - TARGATT™ Site Specific Knock-in Mouse Model Generation Service
  - Do-It-Yourself! Mouse Model Generation Products
  We can also generate large transgene knock-in mouse models (up to 6 kb) using our highly optimized CRISPR/Cas9 technology, where a gene of interest can be inserted at a specific endogenous locus of choice.
  
  Learn more about CRISPR/ Cas9 Mouse Model Generation Service
  
  Site-specific TARGATT™ Mouse Models
  
  Site-Specific Knock-In TARGATT “attP” Mice
  
  TARGATT™ attP mice available at Applied StemCell, along with associated kits enable you to make site-specific transgenic mouse models in your lab.
  
  Site-Specific Knock-In TARGATT “attP” Mice

Mouse Repository

Immune Checkpoint Mouse Models
Coming soon!

Applied StemCell’s off-shelf mouse catalog offers >100 humanized immune checkpoint mouse models, with single, double, and triple humanized gene models.
- Knock-in of humanized counterparts of immune checkpoint gene into the endogenous locus.
- Proprietary human transgene knock-in strategy to enable screening of many different antibodies and immuno-therapy compounds.
- In vivo and in vitro validated for physiological expression of human version of the genes (in-house and through collaborators).
- Fully functional murine immune system
- The humanized immune checkpoint mice have been generated in the most widely used C57BL/6J background used in immunology and oncology studies.
- Ideal for immunology and antibody/ immuno-oncology therapy efficacy studies and drug discovery research.

The Cre-Lox system is a powerful gene manipulation tool that enables controlled gene expression in a specific tissue or by induction in mouse models. Such controlled/ conditional gene expression or ablation enables deeper study of gene function without problems such as artificial phenotype(s) and embryonic lethality.

Applied StemCell offers several ready-to-use, mouse models expressing Cre recombinase under the control of various tissue-specific and/or inducible promoters as well as Cre-reporter mice:

Well-characterized Cre driver mouse lines
Generate temporal and/or spatial control of gene expression mouse models
Breed with your loxP floxed mice or we can generate a custom floxed mouse for you using our PrimeCKo™ Conditional Mouse Model Generation Service; in your facility or ours

Available Cre-Mouse Lines

Cre-Mouse Lines
Alb-(CreERT2)	Cdkn2a-(Luc-tdTomato-CreERT2)
Isl1-(CreERT2)	Pdx1-(Avi-CreERT2)
Map2-(CreERT2)	Lyz2-(CreERT2-EGFP)
Dppa3-(Cre)	Piezo1-(CreERT2)
Cyp2e1-(CreERT2)	Tyr-(CreERT2)
Il17a-(CreERT2)	Slc17a6-(CreERT2)
Myh6-(Cre)	Amhr2-(Cre)
Epcam-(CreERT2)	Cd68-(CreERT2)
H11-(ddCre)	Krt5-(2A-CreERT2)
Kit-CreERT2	Ctsk-(2A-Cre)
Nphs2-CreERT2	Krt14-(CreERT2)
Aplnr-CreXER2	Adgre1-(2A-CreERT2)
Foxl1-CreERT2	Ncr1-(2A-iCre
Hey2-CreERT2	Ly6a-(2A-iCre)
Cdh5-(rtTA-tetO-Cre)	Csf1r-(IRES-iCre
Col1a1-e(TetO-GFPCre-M2rtTA)	Sst-(IRES-Cre)
Drd1-(2A-CreERT2-WPRE-pA)	Crem-KO
Cd2-(2A-CreERT2-WPRE-pA)	Afp-CreERT2
Lyz2-(2A-CreERT2-WPRE-pA)	Amhr2-Cre
Sftpc-(IRES-CreERT2)	Lrat-(2A-Cre)
Zbtb46-(2A-CreERT2-WPRE-pA)	Ucp1-(CreERT2)
Tie1-(2A-Cre)	Epx-(iCre)
Col2a1-(2A-CreERT2)	Ucp1-(Cre)
Nkx3-1-(CreERT2)
Tert-(CreERT2)

Cytokine Mouse Models

Coming soon!

Mouse models with cytokine promoter-driven expression of reporter gene(s) for real-time tracking, imaging of inflammatory factors & immune processes.

Model Name	Genes	Gene Synonyms	Human Orthologs
IL4RA-HU	Il4ra	Il4r; CD124	IL4R
IL17A-HU	Il17a	Il17	IL17A
IL6R-HU	Il6ra	CD126, IL-6R	IL-6R
IL6-HU	Il6	Il-6	IL6
IL9-HU	Il9	P40, Il-9	IL9
IL23A-HU	Il23a	p19, IL-23	IL23A
Il5-HU	Il5	Il-5	IL5
IL17F-HU	Il17f	C87042; IL-17F	IL17F
IL3-HU	Il3	BPA; PSF; HCGF; Il-3; MCGF; Csfmu	IL3
IL6&IL6R-HU	Il6; Il6ra	Il-6; CD126, IL-6R	IL6; IL-6R
IL7-HU	Il7	Il-7; hlb368; A630026I06Rik	IL7
IL3-HU	Il3	BPA, Csfmu, HCGF, Il-3, MCGF, PSF	IL3
IL1B-HU	Il1b	Il-1b; IL-1beta	IL1B
IL2-HU	Il2	IL-2	IL2
IL17RA-HU	Il17ra	Il17r; Cdw217; VDw217; AW538159	IL17RA
IL5RA-HU	Il5ra	Il5r; CD125; CDw125	IL5RA
IL2RA-HU	Il2ra	CD25; Il2r; Ly-43	IL2RA
IL3&CSF2-HU	Il3; Csf2	BPA, Csfmu, HCGF, Il-3, MCGF, PSF; CSF; Csfgm; GMCSF; Gm-CSf; MGI-IGM	IL3; CSF2

Other Gene Edited Mouse Models (KO, CKO)

Coming Soon! Off-shelf human disease models featuring knock-in, knock-out, point mutations, cKI/cKO as well as tool mice for advanced mouse model generation.
Immunodeficient Mouse Models

Coming Soon!

Immunodeficient mouse models (such as M-NSG, Rag1-KO, TCRa and more) for xenografting (PDX, CDX), immuno-oncology, inflammatory & immunology studies.

Rat Models
- PrimeCKO™ Conditional Knockout Rat Models
  
  Applied StemCell can generate a custom conditional knockout rat model using CRISPR/Cas9 or other suitable gene editing techniques to insert the 5’ and 3’ flanking LoxP sequences into the endogenous locus of your gene of interest. These conditional knockout rats can be bred with commercially available Cre expressing rats or custom engineered Cre expressing rats to generate your rat model with precise control over where and when your gene is knocked out.
  
  Learn more about our CRISPR Conditional Knockout Rat Model Generation
  
  ASC offers Bacterial Artificial Chromosomes (BACs) to generate rat models. BACs are ultra-low copy vectors that hold up to 300 kb of genomic fragments.
  
  Learn more about our BAC Conditional Knockout Rat Model Generation
- Site-Specific TARGATT™ Knock-in Rat Models
  Applied StemCell Inc.’s proprietary TARGATT™ technology uses PhiC31 integrase to insert any gene of interest into a docking site that has been pre-engineered into an intergenic and transcriptionally active genomic locus.
  
  Site-Specific Transgenic TARGATT™ Rat Model:
  - High efficiency gene integration (up to 40%)
  - Single copy, large DNA fragment knock-in (up to 22 kb)
  - Site-specific gene insertion into a preselected, safe harbor locus
  - Unidirectional integration
  - Direct injection of the DNA into rat zygotes
  - Non-immunogenic TARGATT™ plasmid
  - Works independent of cell division
  - Sprague-Dawley rats
  - Founder rats in 4-6 months
  - Advantageous in vivo model for researchers seeking better representation of human diseases
- Knockout, Knock-in, Point Mutation Rat Models
  Applied StemCell (ASC) leverages its long-standing expertise in CRISPR/Cas9 technology, as well as animal model engineering to generate rat models with precise gene modifications for a variety of research and preclinical applications.
  
  Key Features:
  - Up-to-date CRISPR design strategies
  - 100% target-site cutting efficiency using validated gRNA
  - Highly successful at generating final rat model
  - ISO:9001 certified facility for manufacturing CRISPR reagents
  - Wide variety of genome modifications available
  - F1 breeding to confirm germline transmission
  - Animal IP belongs to researchers
  - Electrophysiology and behavioral assessments for your rat models
  Learn about our CRISPR Rat Model Generation Service
- Cre Rat Models
  
  These transgenic Cre rat models can enable the developed of physiologically relevant human cardiovascular or neurological diseases using either ASC's proprietary integrase-based TARGATT™ System or CRISPR/cas9 genome editing technology.
  
  These products were supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R44GM108071. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health
Custom In Vivo Assay Services

Animal Models

Mouse Models

PrimeCKO™ Conditional Knockout Mouse Models

Knockout, Knock-in, Point Mutation Mouse Models

Site-Specific TARGATT™ Knock-in Mouse Models

Site-Specific Knock-In TARGATT “attP” Mice

Site-Specific Knock-In TARGATT “attP” Mice

Mouse Repository

Immune Checkpoint Mouse Models

Cre Mouse Models

Cytokine Mouse Models

Other Gene Edited Mouse Models (KO, CKO)

Immunodeficient Mouse Models

Rat Models

PrimeCKO™ Conditional Knockout Rat Models

Site-Specific TARGATT™ Knock-in Rat Models

Knockout, Knock-in, Point Mutation Rat Models

Cre Rat Models

Custom In Vivo Assay Services

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