Cre Mouse Models

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The Cre-Lox system is a powerful gene manipulation tool that enables controlled gene expression in a specific tissue or by induction in mouse models. Such controlled/ conditional gene expression or ablation enables deeper study of gene function without problems such as artificial phenotype(s) and embryonic lethality.

Applied StemCell offers several ready-to-use, mouse models expressing Cre recombinase under the control of various tissue-specific and/or inducible promoters as well as Cre-reporter mice:

  • Well-characterized Cre driver mouse lines
  • Generate temporal and/or spatial control of gene expression mouse models
  • Breed with your loxP floxed mice or we can generate a custom floxed mouse for you using our PrimeCKo™ Conditional Mouse Model Generation Service; in your facility or ours
 

Available Cre Mouse Lines

Cre-Mouse Lines
Alb-(CreERT2) Cdkn2a-(Luc-tdTomato-CreERT2)
Isl1-(CreERT2) Pdx1-(Avi-CreERT2)
Map2-(CreERT2) Lyz2-(CreERT2-EGFP)
Dppa3-(Cre) Piezo1-(CreERT2)
Cyp2e1-(CreERT2) Tyr-(CreERT2)
Il17a-(CreERT2) Slc17a6-(CreERT2)
Myh6-(Cre) Amhr2-(Cre)
Epcam-(CreERT2) Cd68-(CreERT2)
H11-(ddCre) Krt5-(2A-CreERT2)
Kit-CreERT2 Ctsk-(2A-Cre)
Nphs2-CreERT2 Krt14-(CreERT2)
Aplnr-CreXER2 Adgre1-(2A-CreERT2)
Foxl1-CreERT2 Ncr1-(2A-iCre
Hey2-CreERT2 Ly6a-(2A-iCre)
Cdh5-(rtTA-tetO-Cre) Csf1r-(IRES-iCre
Col1a1-e(TetO-GFPCre-M2rtTA) Sst-(IRES-Cre)
Drd1-(2A-CreERT2-WPRE-pA) Afp-CreERT2
Cd2-(2A-CreERT2-WPRE-pA) Amhr2-Cre
Lyz2-(2A-CreERT2-WPRE-pA) Lrat-(2A-Cre)
Sftpc-(IRES-CreERT2) Ucp1-(CreERT2)
Zbtb46-(2A-CreERT2-WPRE-pA) Epx-(iCre)
Tie1-(2A-Cre) Ucp1-(Cre)
Col2a1-(2A-CreERT2)  
Nkx3-1-(CreERT2)  
Tert-(CreERT2)  
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Application Notes

Validation Data for Alb-CreERT2 Mouse Line

Strain Name: B6.129-Albtm1.1(CreERT2)/Asc Strain Background: B6;129

The albumin (Alb) gene encodes albumin, one of the most abundant protein in the blood and is expressed in the liver. The albumin gene promoter regulates transcription in terminally differentiated hepatic tissues and is an effective tool to study gene function in hepatocytes or hepatic tissue. In this Alb-Cre driver mouse line, a tamoxifen-inducible Cre recombinase (CreERT2) was inserted into exons 1-7 in the endogenous albumin locus.

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Figure 1. X-gal staining of liver slides of Alb-CreERT2 and Rosa26-LacZ positive mouse before (- tamoxifen) and after tamoxifen (+-tamoxifen) injection.

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Figure 2. Immunofluorescence analysis of liver using anti-Alb (green) and anti-LacZ (red) antibodies showed that Cre is expressed in most hepatocytes.

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Figure 3. Blood biochemical assay of Alb-CreERT2 male and female mice. Alb-CreERT2 mice do not display any liver functional abnormalities.

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