Newsletter
Bioproduction
The traditional CHO antibody production and animal bioproduction methods are inefficient because they require random gene insertion and forced amplification of transgenes. Applied StemCell has addressed these problems by utilizing our expertise in cell line and animal model engineering with our proprietary TARGATT™ technology to develop two bioproduction methods: TARGATT™ CHO Master Cell Lines and Transgenic TARGATT™ Rabbits. Both methods offer low-cost, efficiency, consistent protein expression and high protein yield that is easily scalable for large scale bioproduction.
Service Options
Transgenic Animals-Rabbit
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Products and Services
Technical Details
Application Notes
CHO Bioproduction
- Safe-Harbor Locus: H11 (non-optimized locus)
- Proteins Expressed: Two therapeutic antibodies, Ab1 and Ab2
- Copies Inserted: 1 (single copy)
- Production Parameters:
- 17-day fed-batch shake-flask
- Bulk enriched pools (no single-cell cloning)
- Yield: ~1 g/l
Biopharming
- Bioproduction in rabbit milk
- TdTomato proof-of-concept
- Tdtomato Molecular weight = 56 kD
- Factor VII molecular weight = 52 kD
- F1 heterozygous rabbit (1 copy)
- Yield: 18g/l in homozygous rabbits
Support Materials
Brochure/ Flyer
Posters:
Publications
- Chi, X., Zheng, Q., Jiang, R., Chen-Tsai, R. Y., & Kong, L. J. (2019). A system for site-specific integration of transgenes in mammalian cells. PLOS ONE, 14(7), e0219842.