• CHO Cells & Transgenic Rabbits
    • TARGATT™ Technology For Bioproduction
    • Consistent Protein Expression and High Protein Yield
    CHO Cells & Transgenic Rabbits

Bioproduction

The traditional CHO antibody production and animal bioproduction methods are inefficient because they require random gene insertion and forced amplification of transgenes. Applied StemCell has addressed these problems by utilizing our expertise in cell line and animal model engineering with our proprietary TARGATT™ technology to develop two bioproduction methods: TARGATT™ CHO Master Cell Lines and Transgenic TARGATT™ Rabbits. Both methods offer low-cost, efficiency, consistent protein expression and high protein yield that is easily scalable for large scale bioproduction.

Service Options

Bioproduction - CHO Cells

Bioproduction: CHO Cells

TARGATT™ CHO Cells for Recombinant Protein and Antibody Bioproduction!

Bioproduction: CHO Cells

Transgenic Animals - Rabbit

Transgenic Animals-Rabbit

TARGATT™ Rabbit for Biopharming and High-Yield Recombinant Protein and Antibody Production

Transgenic Animals-Rabbit

Contact Us

Contact Us

Email: info@appliedstemcell.com

Call: 1-866-497-4180

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Products and Services
Technical Details

Applied StemCell Bioproduction Details

Application Notes

CHO Bioproduction

CHO Bioproduction Service

  • Safe-Harbor Locus: H11 (non-optimized locus)
  • Proteins Expressed: Two therapeutic antibodies, Ab1 and Ab2
  • Copies Inserted: 1 (single copy)
  • Production Parameters:
    • 17-day fed-batch shake-flask
    • Bulk enriched pools (no single-cell cloning)
  • Yield: ~1 g/l

Biopharming

  • Bioproduction in rabbit milk
  • TdTomato proof-of-concept
    • Tdtomato Molecular weight = 56 kD
    • Factor VII molecular weight = 52 kD
  • F1 heterozygous rabbit (1 copy)
  • Yield: 18g/l in homozygous rabbits
Publications
  • Chi, X., Zheng, Q., Jiang, R., Chen-Tsai, R. Y., & Kong, L. J. (2019). A system for site-specific integration of transgenes in mammalian cells. PLOS ONE14(7), e0219842.
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