TARGATT™ HEK293 and CHO Cell Protein Expression

TARGATT™ technology enables the site-specific integration of any gene-of-interest (GOI; up to 20kb) at a preselected safe harbor locus with the use of a unique integrase. Applied StemCell has generated many TARGATT™ cell lines, including the TARGATT™ CHO and HEK293 Master Cell Lines. These high-quality cells are now part of ready-to-use TARGATT™ knock-in kits that you can use to insert your GOI in your own lab. We have observed steady, medium to high levels of protein expression when using our master cell lines for customer projects, so you can confidently purchase any kit that best suits your research needs. Additionally, our experts have used engineered TARGATT™ cells to address common bioproduction issues.

The traditional CHO antibody and animal bioproduction methods are inefficient because they require random gene insertion and forced amplification of transgenes. Applied StemCell has addressed these problems by utilizing our expertise in cell line and animal model engineering with our proprietary TARGATT™ technology to develop two bioproduction methods: TARGATT™ CHO Master Cell Line and Transgenic TARGATT™ Rabbits. Both methods offer low cost, efficiency, consistent protein expression and high protein yield that is easily scalable for large-scale bioproduction.

Advantages of the TARGATT™ Master Cell Line:

• High knock-in efficiency
• Site-specific integration into the H11 genomic hotspot well-defined safe harbor locus
• Single gene knockin: one variant - one locus - one cell line
• Unidirectional integration for stable knock-in cell lines
• Uniform, high-level gene expression
• Overcomes challenges such as random insertion, gene silencing, multiple copy gene integration, ablated gene expression.
• TARGATT™ CHO and HEK293 Master Cell Lines
• Transgenic TARGATT™ Rabbits for Bioproduction

If you would like to work with your own cell line, our experts can engineer the TARGATT™ system into your cell line. To learn more, explore our Custom TARGATT™ Master Cell Line Generation page or contact us today to schedule your free consultation.

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