• Cardiomyocytes Differentiation

Cardiomyocytes Differentiation

Applied StemCell, Inc. provides a comprehensive range of service to differentiate your iPSCs into cardiomyocytes using our proprietary induction protocol and reagents. We deliver ready to use, highly pure (>90%), functional cardiomyocytes. These differentiated cells provide a highly desirable in vitro platform for high content toxicity and drug screening and as a feasible alternative to animal and embryonic stem cell models. 

We can generate differentiated cardiomyocytes from your own pluripotent cell lines derived from healthy or disease iPSCs.

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iPSC Differentiation to Cardiomyocytes

Timelines:

1. Recovery, Expansion and Validation of iPSCs/ESCs  (6 million cells), approx. 3 weeks

2. Cardiomyocytes Differentiation (5 x10^6 cells), approx. 2-4 weeks

3. Characterization of Progenitor Cells/ Differentiated Cells by ICC (2 markers: anti-TNT; anti-SMA staining) 2-4 days

Deliverables:

1. 5 frozen vials of cardiomyocytes (1 million cells/vial). 

2. Report:

  • Phase contrast images of parental iPSC lines provided by the end user after expansion
  • Videos of beating cardiomyocytes differentiated from each parental line
  • ICC images (after thaw) of cardiomyocytes differentiated from each parental line provided by the end user. Cells stained for markers anti-TNT anti-SMA.
  • Freeze-thaw viability and mycoplasma testing

iPSC Differentiation to Blood Lineage Cells:

Ask us about directed differentiation of your iPSCs into hematopoietic stem cells (HSCs) and blood cells. Avoid sourcing problems associated with HSC procurement, and have a consistent and continuous source of mature human blood cells that are otherwise hard to obtain and maintain. 

Applications: hematopoiesis, cancer immunotherapy, autoimmune diseases/ transplant graft rejection, and drug screening.

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!