Photoreceptor Cell Differentiation
Photoreceptor cells, rods and cones, can be found in the retina and are sensitive to low and high levels of light, respectively. Rods and cones take light and generate signals that are sent to the brain where that information can be processed as part of the first steps in vision. Applied StemCell (ASC) uses non-integrating differentiation protocols to produces the photoreceptor cells that best fit your project needs. The lineage-committed photoreceptor cells can express photoreceptor-specific markers, including CRX, NR2, NR2E3, Tuhi1, Rhodopsin, and PPR6a.
To get you the iPSC-derived photoreceptor cells you need, you can send in your healthy or disease iPSC line, or you can request to use one of ASC’s control iPSC lines. If you would like to use a genetically engineered iPSC line for the differentiation of photoreceptor cells, ASC also offers high-throughput iPSC genome editing service.
With a fast turnaround time of 12-15 weeks, ASC is the one-stop shop you need to obtain high-purity photoreceptor cells. Applied StemCell now offers Adeno-Associated Virus (AAV) Infectivity assay service: serotype selection, promoter testing, and MOI optimization. Contact us today to learn more!
Photoreceptor Differentiation Service Can Include:
- iPSC Generation
- iPSC Characterization
- Photoreceptor Differentiation
- Photoreceptor Characterization
*Contact us today to speak with one of our experts who can help you design the project you need to drive your research forward.
Figure 1: Photoreceptor precursors derived from human iPSCs verified by antibody staining (Left: 20x). The cells with cryopreserved at 1 million cells/vial and recovered in culture media. Antibody staining was completed one day post-thaw (Right: 40x).
Figure 2: Early-stage iPSC-derived photoreceptor cell antibody staining (two weeks in stage 3 media + 5 days in stage 4 media).
Figure 3: iPSC differentiated photoreceptor cell antibody staining (different time point in stage 3 media + 5 days in stage 4 media). No significant difference was observed at the different time points.
Figure 4: AAV Transfection. 48 hours after virus transduction (seeded 2x105 cells/cell; MOI: 1x105)
Figure 5: AAV Transfection. 72 hours after virus transduction. (seeded 2x105; MOI: 1x105)