Mouse Models

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Genetically engineered mouse models have been the workhorse of biomedical research and mammalian genetics for several decades. Traditional methods to genetically modify mice have been plagued by a plethora of problems such as low efficiency, high incidence of undesirable outcomes, random insertion or deletion of genes, and gene silencing to name a few. The advent of targeted gene editing technologies using nucleases (CRISPR/Cas9, ZFNs, TALENs) and recombinase (Cre, FLP, PhiC31 integrase), and has greatly improved site-directed mutagenesis and thereby enables the generation of more representative models of human diseases with increased efficiency, and faster turnaround.

Applied StemCell's uses two complementary gene editing technologies, CRISPR/Cas9 and the proprietary site-specific DNA integration platform, TARGATT™ to enable the generation of mouse models with a variety of gene modifications such as knock-in, knockout, point mutation, gene correction, reporter gene insertion, conditional knockout, gene overexpression models that are mimic the human condition better.

Key Features of Applied StemCell’s Mouse Model Generation Platform:

  • Most up-to-date project designing strategies and protocols
  • ISO:9001 certified facility for manufacturing high quality CRISPR reagents for microinjection
  • AAALAC-accredited animal facility in the USA for engineering mouse models
  • Wide variety of genome modifications to generate a mouse model for your research specifications
  • F1 breeding to confirm germline transmission
  • Animal IP belongs to researchers
  • NEW! Electrophysiology and behavioral assessments for your mouse models
  • Dedicated project management and timely milestone and final reports

Some examples of available genetic modifications in mice include but not limited to:

  • Constitutive and conditional knockouts
  • Small fragment insertions, point mutations
  • Large fragment knock-in: locus specific or safe harbor locus
  • Gene tagging/ reporter gene insertion
  • Gene replacement, humanized models
  • Gene fusion/ translocation
  • Gene overexpression, inducible expression, promoter modifications
  • Gene editing/ correction to model human diseases
  • Knockout, Knock-in, Point Mutation Mouse Models

    Applied StemCell leverages its several years of expertise in mouse model engineering and CRISPR/Cas9 technology to custom generate mouse models with gene knockout, knock-in or point mutations to accurately model human diseases and physiology for advancing biomedical research and preclinical studies

  • Conditional Knockout Mouse Model Generation Service

    Conditional Knock-out Mouse Models for a variety of temporal and spatially controlled gene knock-out conditions in mice.

  • Large Transgene Knock-in Mouse Models

    Fast and Reliable! Site-specific knock-in mouse model generation service for safe harbor locus large transgene insertion, gene overexpression and custom cre mouse line generation.

 

Comprehensive Technology Platform for Gene Editing

Methods

Technical Advantage

TARGATT™ phiC31 integrase

  • Site-specific integration (H11 or ROSA26)
  • Works for large DNA Knock-in (~22kb)
  • High efficiency (up to 40%)
  • Insert promoter of choice for gene overexpression/ inducible expression

CRISPR / Cas9

  • High specificity
  • High efficiency in knockout, point mutation, and conditional knockout
  • Ease of use
  • Works for large DNA knock-in (-10kb)

We also offer mouse model generation service using an expanded technology portfolio such as traditional homologous recombination via ESCs, bacterial artificial chromosome and random transgenic technologies. With our expertise in mouse model generation service and various genome editing technologies, we can assure you a custom genetically engineered mouse model perfect for your research needs.

Homologous Recombination

Conditional Knockout & Knock-in Mouse Models

 

Transgenic Mouse Models

Bacterial Artifical Chromosome & Random Microinjection