• Rosa26 Knock-in Mouse Models
    • Large Gene Insertion
    • AAALAC and Worldwide Shipping
    Rosa26 Knock-in Mouse Models

CRISPR Rose26 Knockin Mouse Models

Safe Harbor locus knock-in enables fast and site-specific insertion of genes into an intergenic, transcriptionally active locus such as the well-defined mouse Rosa26 and Hipp11 (H11) loci. It permits the insertion of large transgenes more efficiently than at a specific genetic locus and overcomes problems associated with random insertion such as disruption of the host genome and gene silencing. Safe harbor locus knock-in mouse models are ideal for gene overexpression, gene knockdown, humanized gene knockin, inducible gene expression, and other applications. Applied StemCell’s has a thorough understanding of safe harbor knock-in (Rosa 26, H11, others) with CRISPR, for a fast, site-specific knock-in mouse model generation, including large transgene insertion.

  • Locus specific (or) safe harbor locus knock-in: Rosa26
  • Novel CRISPR strategy for higher efficiency and success rate
  • Efficient CRISPR strategies and protocols for large fragment knock-in
  • F1 breeding for germline transmission
  • Animal generated in an AAALAC-accredited animal facility
  • Animal IP belongs to customers
  • Fast turnaround of 5-8 months
  • Free consultation
  • We also offer a repository of off-shelf mouse models 
Insert Position Strategy Large Size (bp) Positive Rate
Rosa26 CRISPR/Cas9 18454 0.27%
Products and Services
Case Studies

Case Study 1

Knockin Model: Rosa26 Knockin Mouse Model

Rosa26 KI Strategy

Figure 1: Strategy of Rosa26 knockin mouse model

PCR Identification Image 1

PCR Identification Image 2

PCR Identification Image 3

Figure 2Agarose gel electrophoresis of PCR products. NO. 9 was the positive homologous recombinant F0 mouse. (number: the number of F0 mice;M: 1kb DNA ladder from Fermentas)

Case Study 2

Knockin Model: Rosa26 Knockin Mouse Model

R26-e(SA-2xpA-CAG-LSL-Vegfc-IRES-Luc-2A-tdTomato-Wpre-pA)1 mouse model

Knock-in strategy

R26-e(SA-2xpA-CAG-LSL-Vegfc-IRES-Luc-2A-tdTomato-Wpre-pA)1 mouse model - strategy

Figure 1. Targeting strategy for Vegfc gene knock-in

PCR genotyping of homologous recombinant F0 mice

Figure 2. Agarose gel electrophoresis of PCR result. NO.33 was homologous recombinant F0 mice. 5arm: 5’homologous recombinant fragment. 3arm: 3’homologous recombinant fragment. (number: the number of F0 mice; M: 1kb DNA ladder)