• Rosa26 Knock-in Mouse Models
    • Large Gene Insertion: 18454
    • AAALAC and Worldwide Shipping
    Rosa26 Knock-in Mouse Models

Rose26 Kockin Mouse Model

Safe Harbor locus knock-in enables fast and site-specific insertion of genes into an intergenic, transcriptionally active locus such as the well-defined mouse Rosa26 and Hipp11 (H11) loci. It permits the insertion of large transgenes more efficiently than at a specific genetic locus and overcomes problems associated with random insertion such as disruption of the host genome and gene silencing. Safe harbor locus knock-in mouse models are ideal for gene overexpression, gene knockdown, humanized gene knockin, inducible gene expression, and other applications. Applied StemCell’s has a thorough understanding of safe harbor knock-in (Rosa 26, H11, others) with CRISPR, for a fast, site-specific knock-in mouse model generation, including large transgene insertion.

  • Locus specific (or) safe harbor locus knock-in: Rosa26
  • Constitutive or conditional/inducible expression mouse models
  • Novel CRISPR strategy for higher efficiency and success rate
  • Efficient CRISPR strategies and protocols for large fragment knock-in
  • F1 breeding for germline transmission
  • Animal generated in an AAALAC-accredited animal facility
  • Animal IP belongs to customers
  • Fast turnaround of 5-8 months

 

Insert Position Strategy Large Size (bp) Positive Rate
Rosa26 Cas9 18454 0.27%
Products and Services
Case Studies

Case Study 1

Knockin Model: Rosa26 Knockin Mouse Model

Rosa26 KI Strategy

Figure 1: Strategy of Rosa26 knockin mouse model

PCR Identification Image 1

PCR Identification Image 2

PCR Identification Image 3

Figure 2Agarose gel electrophoresis of PCR products. NO. 9 was the positive homologous recombinant F0 mouse. (number: the number of F0 mice;M: 1kb DNA ladder from Fermentas)

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