Newsletter
CRISPR Rose26 Knockin Mouse Models
Safe Harbor locus knock-in enables fast and site-specific insertion of genes into an intergenic, transcriptionally active locus such as the well-defined mouse Rosa26 and Hipp11 (H11) loci. It permits the insertion of large transgenes more efficiently than at a specific genetic locus and overcomes problems associated with random insertion such as disruption of the host genome and gene silencing. Safe harbor locus knock-in mouse models are ideal for gene overexpression, gene knockdown, humanized gene knockin, inducible gene expression, and other applications. Applied StemCell’s has a thorough understanding of safe harbor knock-in (Rosa 26, H11, others) with CRISPR, for a fast, site-specific knock-in mouse model generation, including large transgene insertion.
- Locus specific (or) safe harbor locus knock-in: Rosa26
- Novel CRISPR strategy for higher efficiency and success rate
- Efficient CRISPR strategies and protocols for large fragment knock-in
- F1 breeding for germline transmission
- Animal generated in an AAALAC-accredited animal facility
- Animal IP belongs to customers
- Fast turnaround of 5-8 months
- Free consultation
- We also offer a repository of off-shelf mouse models
| Insert Position | Strategy | Large Size (bp) | Positive Rate |
| Rosa26 | CRISPR/Cas9 | 18454 | 0.27% |
Products and Services
Case Studies
Case Study 1
Knockin Model: Rosa26 Knockin Mouse Model
Figure 1: Strategy of Rosa26 knockin mouse model
Figure 2：Agarose gel electrophoresis of PCR products. NO. 9 was the positive homologous recombinant F0 mouse. (number: the number of F0 mice；M: 1kb DNA ladder from Fermentas）
Case Study 2
Knockin Model: Rosa26 Knockin Mouse Model
R26-e(SA-2xpA-CAG-LSL-Vegfc-IRES-Luc-2A-tdTomato-Wpre-pA)1 mouse model
Knock-in strategy
Figure 1. Targeting strategy for Vegfc gene knock-in
PCR genotyping of homologous recombinant F0 mice
Figure 2. Agarose gel electrophoresis of PCR result. NO.33 was homologous recombinant F0 mice. 5arm: 5’homologous recombinant fragment. 3arm: 3’homologous recombinant fragment. (number: the number of F0 mice; M: 1kb DNA ladder)