iPSC-Derived Retinal Pigment Epithelium (RPE) & Photoreceptor Cells
iPSC-derived Retinal Pigment Epithelium (RPE)
Retinal Pigment Epithelium (RPE) cells form a single layer in the retina that functions as a selective barrier. RPE can also absorb scattered light and participates in phagocytosis. Applied StemCell (ASC) provides off-the-shelf high-purity iPSC-derived retinal pigment epithelium (RPE) cells that express markers, PMEL1, MITF, ZO-1, and RPE65.
iPSC-derived Photoreceptor Cells
Photoreceptor cells, rods and cones, can be found in the retina and are sensitive to low and high levels of light, respectively. Rods and cones take light and generate signals that are sent to the brain where that information can be processed as part of the first steps in vision. ASC also provides off-the-shelf high-purity iPSC-derived photoreceptor cells that express markers, CRX, NR2E3, Tuj1, Rhodopsin, and PDE6a.
These RPE and photoreceptor cells can be used for disease modeling, drug target discovery, and other biomedical and applied research.
Characterization of the ASE-9710 Retinal Pigment Epithelium Cells
Figure 1. Immunostaining of ASE-9710 iPSC-derived Retinal Pigment Epithelium Cells for retinal pigment
epithelium biomarkers. Cryopreserved RPE cells, differentiated from Applied StemCell’s control iPSC line, ASE9211 were recovered in RPE culture media. The cells were stained with RPE markers, PMEL1, MITF, ZO-1 and
Characterization of the ASE-9715 Photoreceptor Cells
Figure 2. Immunostaining of Mature Photoreceptors derived from human iPSCs for photoreceptor biomarkers. Photoreceptor precursors (ASE-9715), derived from Applied StemCell’s control iPSC line, ASE9211 can be further differentiated in photoreceptor maturation media in 1-2 weeks. The mature photoreceptors were verified by antibody staining with photoreceptor markers, Tuji, Rhodopsin, and PDE6a.