iPSC-Derived Retinal Pigment Epithelium (RPE) & Photoreceptor Cells
With our optimized differentiation protocols, we produce high-quality cells and deliver well-characterized iPSC-derived photoreceptor and retinal pigment epithelium (RPE) cells for your disease modeling, drug target discovery, and other biomedical and applied research.
iPSC-Derived Photoreceptor Cells
Photoreceptor cells, rods and cones, can be found in the retina and are sensitive to low and high levels of light, respectively. Rods and cones take light and generate signals that are sent to the brain where that information can be processed as part of the first steps in vision. Applied StemCell (ASC) provides off-the-shelf, high-purity iPSC-derived photoreceptor cells that express markers, CRX, NR2E3, Tuj1, Rhodopsin, and PDE6a.
iPSC-Derived Retinal Pigment Epithelium Cells
Retinal Pigment Epithelium (RPE) cells form a single layer in the retina that functions as a selective barrier. RPE can also absorb scattered light and participates in phagocytosis. ASC supplies retinal pigment epithelium (RPE) cells that express markers, PMEL1, MITF, ZO-1, and RPE65.
|Retinal Pigment Epithelium||Photoreceptor Cells|
Do you have your own iPSC you would like to differentiate? ASC can use your iPSCs or an ASC Control iPSC Line to produce the photoreceptor or RPE cells you need. We can even develop any assay you may need for your research.
Products and Services
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Case Study 1
Characterization of iPSC-derived ASE-9715 Photoreceptor Cells
Figure 1. Immunostaining of Mature Photoreceptors derived from human iPSCs for photoreceptor biomarkers. Photoreceptor precursors (ASE-9715), derived from Applied StemCell’s control iPSC line, ASE9211 can be further differentiated in photoreceptor maturation media in 1-2 weeks. The mature photoreceptors were verified by antibody staining with photoreceptor markers, Tuji, Rhodopsin, and PDE6a.
Case Study 2
Characterization of iPSC-derived ASE-9710 RPE Cells
Figure 2. Immunostaining of ASE-9710 iPSC-derived Retinal Pigment Epithelium Cells for retinal pigment
epithelium biomarkers. Cryopreserved RPE cells, differentiated from Applied StemCell’s control iPSC line, ASE9211 were recovered in RPE culture media. The cells were stained with RPE markers, PMEL1, MITF, ZO-1 and
What other characterizations have you done on iPSC-RPE (e.g., tight junction measurement, Zo-1 staining, POS phagocytosis, ABCA4 measurement, VEGF and PEDF secretion, retinoid profiling, microvilli at the apical side)?
We did ZO-1 staining to stain the tight junction. We have not tried the others mentioned. These can be tested upon your request.
As specified in the ASE-9710 datasheet, our cells were observed very differently from the picture shown on the datasheet.
It takes a while for the cells to show the typical morphology and form tight junctions, depending on the starting density. Keep feeding the cells for 1-2 more weeks AFTER they are 100% confluent.
Have the scientists done iPSC-RGC cells or other photoreceptor cells? Which type and what characterization?
We did Rod cells and it was pretty successful. We used CRX and NR2E3 to characterize precursors and Rhodopsin and PDE6a to characterize mature cells. We have not done others, but we can try RGC.
Photoreceptor Cells: Which percentage are ROD cells? I see a RHO staining but few cells are RHO positive? What cell type are the other cells?
We do not know what the other cell type is without staining. We do see about 90% of the cells labeled by RHO.
RPE: How many passages can be done in these cells? What is their doubling time? Do you have contamination of other cell types and if yes, in which proportion?
The most we did was two passages, and doubling time is about 7 days. Our protocol generates a near homogenous population of RPE (>90%).
Could we proliferate the photoreceptor cells ?
Are the cells named Photoreceptor Cells (ASE-9715) photoreceptor precursors cells, and media from Applied StemCell (Photoreceptor Maturation Basal Media) differentiate the cell to maturated photoreceptor?
Yes. We only provide Photoreceptor precursors, and Photoreceptor precursors can be further differentiated into matured photoreceptors using media provided with the kit (Photoreceptor Maturation Basal Media + Photoreceptor Maturation Media supplement).
Is there maintenance media for maturated photoreceptors?
The same media provided in the kit can be used for the maintenance of maturated photoreceptors.
Can the maturated photoreceptors be passaged or stocked in cryovials?
No, maturated photoreceptors cannot be passaged nor cryopreserved.
RPE: If we decide to try to grow some cells out a passage or two should we just use trypsin to release them or do you recommend some other reagent for detachment?
We use TrypLE. Trypsin and any other reagents like Accutase should work as well.
RPE: What are the recommended cell densities at different well sizes?
It depends on the downstream assays. Denser density (100,000-150,000 cells/cm2) is recommended to have cells pigmentated and form tight junctions sooner.
In the data sheet it says RPE can be passaged, how many passages are possible?
The most we have done is two passages. Our protocol generates a near homogenous population of RPE (>90%).
Maturity of RPE, how long it take to get pigmented cells?
It depends on the seeding density. Once it is 100% confluent, it may take 2-3 more weeks for pigmentation.
Is there just one media or you recommend different media for RPE maturation?
You only need the one media provided in the kit for 1 mil to be cultured for 7 days.
Any other details you could please share on the RPE?
We have completed a few RPE projects, some of which included iPSC genome editing.