What other characterizations have you done on iPSC-RPE (e.g., tight junction measurement, Zo-1 staining, POS phagocytosis, ABCA4 measurement, VEGF and PEDF secretion, retinoid profiling, microvilli at the apical side)?
We did ZO-1 staining to stain the tight junction. We have not tried the others mentioned. These can be tested upon your request.
Have the scientists done iPSC-RGC cells or other photoreceptor cells? Which type and what characterization?
We did Rod cells and it was pretty successful. We used CRX and NR2E3 to characterize precursors and Rhodopsin and PDE6a to characterize mature cells. We have not done others, but we can try RGC.
Photoreceptor Cells: Which percentage are ROD cells? I see a RHO staining but few cells are RHO positive? What cell type are the other cells?
We do not know what the other cell type is without staining. We do see about 90% of the cells labeled by RHO.
RPE: How many passages can be done in these cells? What is their doubling time? Do you have contamination of other cell types and if yes, in which proportion?
The most we did was two passages, and doubling time is about 7 days. Our protocol generates a near homogenous population of RPE (>90%).
Could we proliferate the photoreceptor cells ?
Are the cells named Photoreceptor Cells (ASE-9715) photoreceptor precursors cells, and media from Applied StemCell (Photoreceptor Maturation Basal Media) differentiate the cell to maturated photoreceptor?
Yes. We only provide Photoreceptor precursors, and Photoreceptor precursors can be further differentiated into matured photoreceptors using media provided with the kit (Photoreceptor Maturation Basal Media + Photoreceptor Maturation Media supplement).
Is there maintenance media for maturated photoreceptors?
The same media provided in the kit can be used for the maintenance of maturated photoreceptors.
Can the maturated photoreceptors be passaged or stocked in cryovials?
No, maturated photoreceptors cannot be passaged nor cryopreserved.
"iPSC-Derived Human Photoreceptor Kit (African-American, Male Line) *10 kit minimum", does this mean I have to buy 10 vials at one time?
Yes, 10 vials (kits) need to be ordered. Production time is about 8 weeks.