TARGATT™ Master iPSCs and Knockin Kit for Site-specific Transgene Knockin
The TARGATT™ Master iPSC line is engineered from our well-characterized control human iPSC line, ASE-9211 (a NIST iPSC). The master cell line carries an “attP” integrase recognition landing pad in the H11 safe harbor locus. When used in conjunction with an “attB” containing donor plasmid and integrase expression, this master cell line enables site-specific integration of transgene (up to 20kb) with very high efficiency.
- Higher efficiency than other comparable technologies
- Site-specific: well-defined, transcriptionally active safe harbor locus
- Unidirectional integration
- Single copy integration
- A valuable tool for generating reporter knock-in lines, for directed differentiation and gene overexpression in iPSCs.
Products and Services
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When and what plasmid map sequence information is provided for the AST-1600 kit?
The plasmid map, partial sequence will be disclosed upon purchase, so that you can construct a cloning vector, though we are not disclosing the landing pad sequences, nor the entire plasmid sequence.
What is the recommended selection strategy for the AST-1600 kit?
Selection strategy using hygromycin and ganciclovir (recommended): The ganciclovir (GCV) treatment (negative selection) and hygromycin treatment (positive selection) can remove the non-transfected cells and off-target cells as well as enrich for cells with transgene integrated into the H11 locus.
How much integrase is included in the AST-1600 kit?
15 µg of the AST-3071: TARGATT™ CMV-integrase Plasmid
Can we purchase additional integrase?
Yes, additional integrase is available for purchase.