iPSC-derived Motor Neurons
Applied StemCell provides high quality, motor neuron precursors differentiated from well-characterized, integration-free, control human iPSC lines from multiple donors. Our proprietary differentiation protocol uses an efficient, integration-free, small molecule-based method along with an optimized motor neuron culture media (ASE-9701MM) to culture high-purity (≥90%) and robust mature motor neurons. These cells show distinct neurite outgrowth and express late-stage motor neuron precursor biomarkers (HB9, ChAT), and Tuj1 and MAP2 after maturation. These functionally viable, motor neurons are ideal as control lines to compare phenotype and functionality of patient-derived and genome edited iPSC-derived motor neurons, for co-culture model development, and for neurotoxicity and drug screening.
iPSC-derived Motor Neurons: ASE-9701 (From Control Male iPSC Line, ASE-9211) and ASE-9702 (From Control Female iPSC, ASE-9209)
Motor neurons were differentiated from control iPSC lines, ASE-9211 and ASE-9209 using proprietary, integration-free protocols.
Figure 1. Immunocytochemical staining images of the motor neuron derived from control "master" iPSC lines, ASE-9211 (top row) and ASE-9209 (bottom row) at 2 days post thaw. The iPSC-derived motor neurons were stained with antibodies for late-stage motor neuron precursor biomarker, HB9 (green), and neuronal biomarker, Tuj1 (red). DAPI (blue) was used as nucleus stain.
Figure 2. Immunocytochemical staining for mature motor neuron marker, ChAT (green) and neuronal marker, MAP2 (red) in motor neurons differentiated from control "master" iPSC lines, ASE-9211 (top row) and ASE-9209 (bottom row). DAPI (blue) was used for nucleus staining.