iPSC-Derived Motor Neurons
Applied StemCell (ASC) provides high-quality, motor neuron precursors differentiated from well-characterized, integration-free, control human iPSC lines from multiple donors. Our proprietary differentiation protocol uses an efficient, integration-free, small molecule-based method along with an optimized motor neuron culture media (ASE-9701MM) to culture high-purity (≥90%) and robust mature motor neurons. These cells show distinct neurite outgrowth and express late-stage motor neuron precursor biomarkers (HB9, Tuji), and ChAT and MAP2 after maturation. These functionally viable, motor neurons are ideal as control lines to compare the phenotype and functionality of patient-derived and genome-edited iPSC-derived motor neurons, for co-culture model development, and for neurotoxicity and drug screening.
- ≥90% Purity
- Express biomarkers HB9, ChAT, Tuj1, and MAP2
- GMP iPSC Products & Services >> Learn More
Do you have your own iPSC line? We also offer custom iPSC differentiation services. Our experts can differentiate your cells or one of our iPSC control lines into the motor neurons you need. Explore our complete iPSC platform or contact us today to learn more.
Products and Services
Viewing 1-2 of 2 products
Case Study 1
Characterization of iPSC-derived Motor Neurons: ASE-9701 (From Control Male iPSC Line, ASE-9211) and ASE-9702 (From Control Female iPSC, ASE-9209)
Motor neurons were differentiated from control iPSC lines, ASE-9211 and ASE-9209 using proprietary, integration-free protocols.
Figure 1. Immunocytochemical staining images of the motor neuron derived from control "master" iPSC lines, ASE-9211 (top row) and ASE-9209 (bottom row) at 2 days post thaw. The iPSC-derived motor neurons were stained with antibodies for late-stage motor neuron precursor biomarker, HB9 (green), and neuronal biomarker, Tuj1 (red). DAPI (blue) was used as nucleus stain.
Figure 2. Immunocytochemical staining for mature motor neuron marker, ChAT (green) and neuronal marker, MAP2 (red) in motor neurons differentiated from control "master" iPSC lines, ASE-9211 (top row) and ASE-9209 (bottom row). DAPI (blue) was used for nucleus staining.
How many days do I culture the cells until we reach a mature MN?
These high-purity (≥90%) cells show distinct neurite outgrowth in 2-7 days after thaw (Figure 1) and express late stage motor neuron precursor biomarkers, HB9 and ChAT at day 2 after thaw (Figure 2), and mature motor neuron biomarkers, Tuj1 and MAP2 at day 5 (Figure 3). The cells are provided as cryopreserved, late-stage precursors that mature in 5 days after recovery.
How many days can you culture these cells after they are in a mature MN state?
We recommend using the recovered motor neurons within 7-10 days after recovery. Prolonged culture will give rise to non-neuronal cell outgrowth.