• iPSC lines
    • Well characterized control lines
    • Male/ female
    iPSC lines

iPSC Lines, Human, Normal

Applied StemCell offers well-characterized, normal human induced pluripotent stem cell (iPSC) that have been reprogrammed from healthy human tissues (cord blood, fibroblasts) using footprint-free methods. All iPSC lines are carefully selected for morphology of undifferentiated cells, and express pluripotency markers (OCT4, SOX2, SSEA4, and others) and strong endogenous alkaline phosphatase activity. Our normal human iPSCs have proven genome editing and differentiation capacity, and are ideal for disease modeling, drug discovery/screening and neurotoxicity screening applications.

Our “master” iPSC line, ASE-9211 is being used by the NIST genome editing consortium as benchmark material to establish measurements and standards for characterizing genome editing outputs.



iPSC Lines
Episomal iPS Cells (iPSCs from Fibroblast, African American, Male, Neonatal, Episomal); NIST Line *Academic Price 
Episomal iPS Cells (iPSCs from Fibroblasts, Caucasian, Female, Age 47, Episomal) *Academic Price
Episomal iPS Cells (iPSCs from Cord Blood Cells CD34+, Caucasian, Male, Episomal); Master Lines for Neural Differentiation *Academic Price
Episomal iPS Cells (iPSCs from Cord Blood Cells CD34+, Caucasian, Female, Episomal); Master Lines for Neural Differentiation *Academic Price
Inquire iPS Cells (iPSCs from PBMC, Caucasian, Male, Age 21) * Academic Price


cGMP Grade iPS Cells (ASE-9250)

Applied StemCell’s cGMP Grade iPSC Line was generated from male, CD34+ cord blood using an episomal reprogramming method. Master cell bank or working cell bank available.

Also, contact us today to learn more about Applied StemCell's iPS Cells Research Grade (iPSCs from CD34+ Cord Blood, Male).

What is donor information for the ASE-9101 (iPSC from fibroblast, Male, Retrovirus)?
What was the reprogramming method and reprogramming factors used for the generating the control iPSC line ASE-9109?
What is the donor information for the control iPSC line, ASE-9109?
What is the donor information for the control iPSC, ASE-9110?
ASE-9211: Is it possible to thaw them on a Vitronectin XF ECM, or on an hESC-qualified Geltrex basement matrix?
Products and Services
Application Notes

Neural Stem Cell (NSC) Differentiation from Control iPSC, ASE-9109


Figure 1. Neural stem cells (NSC) differentiated from control human iPSC ASE-9109 express NSC markers: SOX1 (red) and NESTIN (green). DAPI (blue): nuclear counterstain.

Motor Neuron Differentiation from Applied StemCell's Master iPSC Line, ASE-9211

Control iPSC line, ASE-9211 was used for differentiation into motor neurons using proprietary, integration-free protocols. 


Figure 2. Immunocytochemical staining for motor neuron marker, ChAT (green) and neuronal marker, MAP2 i(blue) n motor neurons differentiated from control "master" iPSC line, ASE-9211 at 7 days post thaw. DAPI (blue) was used for nucleus staining.


Control iPSC Lines:

  • Tanaka, H., Homma, H., Fujita, K., Kondo, K., Yamada, S., Jin, X., ... & Atsuta, N. (2020). YAP-dependent necrosis occurs in early stages of Alzheimer’s disease and regulates mouse model pathology. Nature Communications, 11(1), 1-22
  • Su, S., Guntur, A. R., Nguyen, D. C., Fakory, S. S., Doucette, C. C., Leech, C., ... & Sims-Lucas, S. (2018). A renewable source of human beige adipocytes for development of therapies to treat metabolic syndrome. Cell reports25(11), 3215-3228.
  • Lizarraga, S. B., Maguire, A. M., Ma, L., van Dyck, L. I., Wu, Q., Nagda, D., ... & Cowen, M. H. (2018). Human neurons from Christianson syndrome iPSCs reveal allele-specific responses to rescue strategies. bioRxiv, 444232.
  • Tanaka, H., Kondo, K., Chen, X., Homma, H., Tagawa, K., Kerever, A., ... & Fujita, K. (2018). The intellectual disability gene PQBP1 rescues Alzheimer’s disease pathology. Molecular Psychiatry, 1.
  • Kavyasudha C., Macrin D., ArulJothi K.N., Joseph J.P., Harishankar M.K., Devi A. (2018) Clinical Applications of Induced Pluripotent Stem Cells – Stato Attuale. In: Advances in Experimental Medicine and Biology. Springer, New York, NY. https://doi.org/10.1007/5584_2018_173.
  • Lin, Y., Linask, K. L., Mallon, B., Johnson, K., Klein, M., Beers, J., ... & Zou, J. (2017). Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin‐Free Medium, Enabling Consistent Manufacture of Cardiomyocytes. Stem cells translational medicine6(2), 527-538.
Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!