• iPSC & ESC Lines

iPSC Lines, Human, Normal

Applied StemCell offers normal human iPSCs reprogrammed from healthy human tissues (cord blood, fibroblasts, PBMCs) using footprint-free methods. All induced pluripotent stem cell (iPSC) lines were derived using morphological selection criteria, and without the use of fluorescent marker or drug selection. The cells express pluripotency markers OCT4, SOX2, SSEA4, and others, and demonstrate strong endogenous alkaline phosphatase activity. Our normal human iPSCs can be used for disease modeling, genome editing serviceneural lineage differentiation, and neurotoxicity screening applications.

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Application Notes

Differentiation to Neural Lineage

appnote-ase-9109-to-NSC

Figure 1. Neural stem cells (NSC) differentiated from control human iPSC ASE-9109 express NSC markers: SOX1 (red) and NESTIN (green). DAPI (blue): nuclear counterstain.

Pluripotency Marker Analysis

webpage-ASE-9209-IHC-marker

Figure 2. Expression of pluripotency markers. ASC 9209 iPS cell line expresses common iPSC biomarkers (OCT4, SOX2, SSEA4, TRA-1-60,  and  TRA-1-81).  The corresponding DAPI staining is below each  marker staining image.  All images were taken at 10x magnification.

FAQs
What is donor information for the ASE-9101 (iPSC from fibroblast, Male, Retrovirus)?
Publications

ASE-9109: Normal iPSC differentiation to cardiomyocytes

  • Kavyasudha C., Macrin D., ArulJothi K.N., Joseph J.P., Harishankar M.K., Devi A. (2018) Clinical Applications of Induced Pluripotent Stem Cells – Stato Attuale. In: Advances in Experimental Medicine and BiologySpringer, New York, NYhttps://doi.org/10.1007/5584_2018_173.
  • Lin, Y., Linask, K. L., Mallon, B., Johnson, K., Klein, M., Beers, J., ... & Zou, J. (2017). Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin‐Free Medium, Enabling Consistent Manufacture of Cardiomyocytes. Stem cells translational medicine6(2), 527-538.

ASE-9028: Sporadic Parkinson’s disease line

  • Hsieh, C. H., Shaltouki, A., Gonzalez, A. E., da Cruz, A. B., Burbulla, L. F., Lawrence, E. S., ... & Wang, X. (2016). Functional impairment in Miro degradation and mitophagy is a shared feature in familial and sporadic Parkinson’s disease. Cell Stem Cell19(6), 709-724.

iPSC-derived cardiomyocyte

  • Daily, N. J., et al. (2017). High-Throughput Phenotyping of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Neurons Using Electric Field Stimulation and High-Speed Fluorescence Imaging. ASSAY and Drug Development Technologies. 15(4): 178-188. https://doi.org/10.1089/adt.2017.781
  • Daily, N. J., Santos, R., Vecchi, J., Kemanli, P., & Wakatsuki, T. (2017). Calcium transient assays for compound screening with human iPSC-derived cardiomyocytes: Evaluating new tools. Journal of evolving stem cell research, 1(2), 1.
  • Daily, N. J., et al. (2015). Journal of Bioengineering & Biomedical Science, 2015.
Have Questions?

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