iPSC Lines, Human, Normal
Applied StemCell offers normal human iPSCs reprogrammed from healthy human tissues (cord blood, fibroblasts, PBMCs) using footprint-free methods. All induced pluripotent stem cell (iPSC) lines were derived using morphological selection criteria, and without the use of fluorescent marker or drug selection. The cells express pluripotency markers OCT4, SOX2, SSEA4, and others, and demonstrate strong endogenous alkaline phosphatase activity. Our normal human iPSCs can be used for disease modeling, genome editing service, neural lineage differentiation, and neurotoxicity screening applications.
Neural Stem Cell (NSC) Differentiation from Control iPSC, ASE-9109
Figure 1. Neural stem cells (NSC) differentiated from control human iPSC ASE-9109 express NSC markers: SOX1 (red) and NESTIN (green). DAPI (blue): nuclear counterstain.
Motor Neuron Differentiation from Applied StemCell's Master iPSC Line, ASE-9211
Control iPSC line, ASE-9211 was used for differentiation into motor neurons using proprietary, integration-free protocols.
Figure 2. Immunocytochemical staining for motor neuron marker, ChAT (green) and neuronal marker, MAP2 i(blue) n motor neurons differentiated from control "master" iPSC line, ASE-9211 at 7 days post thaw. DAPI (blue) was used for nucleus staining.
The male iPSC ASE-9109 was generated from fibroblasts obtained from a healthy, male neonate using retroviral reprogramming methods.
ASE-9109: Normal iPSC differentiation to cardiomyocytes
- Kavyasudha C., Macrin D., ArulJothi K.N., Joseph J.P., Harishankar M.K., Devi A. (2018) Clinical Applications of Induced Pluripotent Stem Cells – Stato Attuale. In: Advances in Experimental Medicine and Biology. Springer, New York, NY. https://doi.org/10.1007/5584_2018_173.
- Lin, Y., Linask, K. L., Mallon, B., Johnson, K., Klein, M., Beers, J., ... & Zou, J. (2017). Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin‐Free Medium, Enabling Consistent Manufacture of Cardiomyocytes. Stem cells translational medicine, 6(2), 527-538.
ASE-9028: Sporadic Parkinson’s disease line
- Hsieh, C. H., Shaltouki, A., Gonzalez, A. E., da Cruz, A. B., Burbulla, L. F., Lawrence, E. S., ... & Wang, X. (2016). Functional impairment in Miro degradation and mitophagy is a shared feature in familial and sporadic Parkinson’s disease. Cell Stem Cell, 19(6), 709-724.
- Daily, N. J., et al. (2017). High-Throughput Phenotyping of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Neurons Using Electric Field Stimulation and High-Speed Fluorescence Imaging. ASSAY and Drug Development Technologies. 15(4): 178-188. https://doi.org/10.1089/adt.2017.781
- Daily, N. J., Santos, R., Vecchi, J., Kemanli, P., & Wakatsuki, T. (2017). Calcium transient assays for compound screening with human iPSC-derived cardiomyocytes: Evaluating new tools. Journal of evolving stem cell research, 1(2), 1.
- Daily, N. J., et al. (2015). Journal of Bioengineering & Biomedical Science, 2015.