DR4 MEF Feeder Cells
Derived from DR4 mouse embryos and are resistant to Neomycin, Hygromycin, Puromycin and 6-thioguanine.
MEF cells serve as feeder cells to support the growth of undifferentiated mouse or human embryonic stem Cells (ESCs) and induced pluripotent stem cells (iPSCs). MEF cells are isolated from mouse embryos and should be used at an early passage. Before use as feeder cells, MEF cells must be mitotically inactivated by γ-irradiation or mitomycin-C treatment.
DR4 MEF feeder cells are are derived from 13.5 day old mouse embryos that are genetically engineered to contain resistance genes to neomycin, hygromycin, puromycin and 6-thioguanin and are optimized for the culture of ES/iPS cells under drug selection.
All MEF cells are manufactured in the US and have an average viability of 95% after thawing. We offer both untreated cells for further expansion and treated cells that can be directly used as a feeder layer.
The DR4 MEFs are derived from pooled embryos.
The DR4 MEFs stop growing after P4. We recommend to use these untreated DR4 MEFs only up to P3.
The doubling time for the untreated DR4 MEF cells is 3 days when split in a ratio of 1:3 until passage 3.
DR4 MEF Feeder Cells
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