DR4 MEF Feeder Cells

Derived from DR4 mouse embryos and are resistant to Neomycin, Hygromycin, Puromycin and 6-thioguanine.

MEF cells serve as feeder cells to support the growth of undifferentiated mouse or human embryonic stem Cells (ESCs) and induced pluripotent stem cells (iPSCs). MEF cells are isolated from mouse embryos and should be used at an early passage. Before use as feeder cells, MEF cells must be mitotically inactivated by γ-irradiation or mitomycin-C treatment.

DR4 MEF feeder cells are are derived from 13.5 day old mouse embryos that are genetically engineered to contain resistance genes to neomycin, hygromycin, puromycin and 6-thioguanin and are optimized for the culture of ES/iPS cells under drug selection.

All MEF cells are manufactured in the US and have an average viability of 95% after thawing. We offer both untreated cells for further expansion and treated cells that can be directly used as a feeder layer.

Also be sure to see our CF1 MEF Feeder Cells, Neo-resistant MEF Feeder Cells, and SNL 76/7 (STO Cell Line) MEF Feeder Cells.

DR4

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FAQs
Do the vials of DR4 MEFs (ASF-1015) contain cells from pooled embryos or is each vial from a single embryo?
How many passages can the DR4 MEFs, ASF-1001/ASF-1002 (Untreated P2) be passaged for?
How many vials of cells can you get from the untreated DR4 MEFs, ASF-1001/ASF-1002 (Untreated P2)?
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DR4 MEF Feeder Cells

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