Products

Applied StemCell’s iPSC catalog includes a broad range of iPS cell lines: footprint-free (episomal) and retroviral iPSC lines from different tissues (PBMCs, fibroblasts, cord blood, adipocytes) from healthy and diseased patients; iPSCs derived from mouse, pig, guinea pigs using episomal or retroviral reprogramming methods, and various mouse ESC lines that have been tested for successful germline transmission. These cells are ideal for all iPS cell culture applications such as disease modeling, differentiation into cellular lineages of choice and drug toxicity/efficacy screening.

We offer fully characterized iPSC-differentiated progenitors and differentiated cell lines derived from integration-free iPSCs using feeder-free culture protocols: Neural stem cells (NSCs), Neurons (Dopaminergic and Cortical neurons), Astrocytes, PBMCs and Cardiomyocytes.

ASC’s gene edited iPS Cell Line catalog includes isogenic panels of gene edited lines from control iPSC line (ASE-9109) that are fully characterized and are ideal for disease modeling: (1) neuronal disease-specific isogenic knockout line; (2) lineage-specific reporter gene knock-in lines; (3) safe harbor locus reporter gene knock-in iPS lines.

Human EZ- iPSC Generation kits - Reprogram your somatic cells to iPSC with the Retroviral or the Episomal kit.

Use Applied StemCell's stem cell validated mouse embryonic fibroblasts (MEF) cells, FBS, 3D media, serum/ feeder-free (SFFM) medium, and supplements, for optimal and robust growth of your stem cell (iPSCs and ESCs) lines. 

Stem Cell Marker kits. Characterization of embryonic stem cells and induced pluripotent stem cells by pluripotency protein or RNA marker kits and stem cell gene arrays.

The CRISPRCLEAR™ kits for cell line model generation are a do-it-yourself kits for affordable and efficient cell line model generation. A variety of kits are available for gene knockout, point mutation and knock-in models in human and mouse cell lines.

In vivo-grade, injection-ready CRISPR kits for the generation of transgenic mice and rats. 

ASC’s proprietary TARGATT™ transgenic mouse model generation products offer an affordable do-it-yourself option to generate site-specific transgenic mice very efficiently and in as little as 3 months, in your own animal facility. You can purchase all required components to generate your transgenic "knock-in" mice right here from our ASC website: TARGATT™ mice, TARGATT™ plasmids, transgenic kits for site-specific knock-in of your transgene, and genotyping kits. The TARGATT™ products are manufactured in our ISO:9001 quality management system certified facility.

Transgenic core facilities currently using the TARGATT™ system, including UCSF, NIH, MaxPlank, Harvard, Colombia and many more!

Rapid generation of site-directed knock-in cell lines.

Applied StemCell, Inc., offers a large selection of gene-targeting products including TARGATT™ Site-specific Knock-in Cell Line. The cell line kit includes a master cell line with a TARGATT™ docking site (attP) at a transcriptionally active locus (the Hipp11 locus on chromosome 11; H11), and vectors for TARGATT™ integrase and DNA integration. With this method, large DNA fragments can be inserted at the H11 locus at high efficiency with robust transgene expression.

Cell Lines expressing the CRISPR Cas9 nuclease enable you to carry out CRISPR genome editing applications including cell based assays and drug discovery.  

ASC’s Gene Edited iPS Cell Line catalog includes isogenic panels of reporter knock-in iPSC lines from a fully characterized control/ master iPSC (ASE-9109). These reporter iPSC lines are fully characterized and are ideal for studying neurological mechanisms and drug testing: (1) Neuronal lineage-specific reporter gene knock-in lines; (2) Safe harbor locus reporter gene knock-in iPS lines.

Isogenic Gene knockout (KO) iPSCs engineered from control iPSC line (ASE-9109), that are fully characterized and are ideal for neurological disease modeling.

Eliminate time consuming substrate injection with Luciferase. We can quickly modify your cell lines to express an autobioluminescent reporter and return them to your laboratory for in vivo imaging. 4 major cell lines (HCT116, HEK293, MCF7, T47D) engineered to be autonomously bioluminescent. Custom Cell Line Developement: we can also quickly modify your cell lines to express an autobioluminescent reporter and return them to your laboratory for in vivo imaging as well. 

MyEZGel CDX/PDX is a protein based solubilized base membrane synthesized from commonly used amino acids, and minerals.  The amino acids include Arginine, Aspartate, Glycine, Isoleucine, Leucine, Lysine, Phenylalanine, Proline, Serine, and Valine. MyEZGel CDX/PDX is compatible with various adhesion proteins and cell growth factors, including but not limited to laminin, collagens, VE-growth factor, fibroblast growth factor, and other growth factors. MyEZGel CDX/PDX is a powerful reagent to package and deliver cancer cells, primary cancer cells, or stem cells in vivo for xenograft animal models.

Human Immortalized Fibroblasts (SV40).

Routine monitoring of mycoplasma is an essential and neccessary quality control procedure for all cell culture labs. ASC's PCR Mycoplasma Detection Kit is a very affordable, sensitive and straightforward kit for monitoring and ensuring your cell lines are mycoplasma-free.