Products

Applied StemCell Products

Applied StemCell Inc. (ASC) offers a large array of products for site specific transgenics, cell culture, and stem cell research. Our products encompass a wide spectrum, from: high quality transgenic kits for knock-in mice and knock-out mice generation, mouse embryonic fibroblast (MEF cells), feeder cells, growth factors, options for stem cell characterization and stem cell differentiation, as well an array of iPSC cells. See below for our full product list.

  • Stem Cell

    • iPSC Cells


      Applied StemCell’s iPSC catalog includes well-characterized, human iPS cell lines suitable for iPS cell culture applications such as genome editing for disease modeling, for differentiation into cellular lineages of choice and drug toxicity/ efficacy screening:

      • Footprint-free iPSC cells (episomal and other integration-free methods) and retroviral-reprogrammed iPSCs
      • Characterized for pluripotency markers, karyotyping and differentiation potential; more characterization options are available
      • Multiple donor tissues: PBMCs, fibroblasts, cord blood, adipocytes from healthy and diseased patients
      • Tested for CRISPR genome editing and differentiation potential into various cell lineages
      • Ideal as master iPSC cell lines to build engineered and/or panels of differentiated isogenic cell lineages
      iPSC & ESC Lines Categories

      iPSC Lines, Human, Normal

      High quality human iPSC lines (hiPSCs) derived from male and female cord blood cells, fibroblasts, and PBMCs, for genome editing and differentiation.

      iPSC Lines, Human, Normal

      iPSC Cells, Human, Disease

      Patient iPSC lines derived from various somatic cells of patients with Parkinson’s disease and Diabetes (II).

      iPSC Cells, Human, Disease

    • iPSC Differentiated Cells


      We offer fully characterized progenitors and differentiated cell lines derived from integration-free iPSCs using feeder-free culture protocols: Neural stem cells (NSCs), Neurons (Dopaminergic and Cortical neurons), Astrocytes, and Cardiomyocytes.

      The cell lines have been characterized for the expression of characteristic biomarkers using immunohistochemistry and for functional viability. Our isogenic panel of neural lineage cells also have been validated by whole genome profiling to ensure cellular integrity and quality.

      These cell lines are ideal for various applications in biomedical and applied research:

      • Disease Modeling
      • Drug target discovery
      • Neurotoxicity and cardiotoxicity drug screening
      • Drug efficacy testing for new neurological drug candidates
      • Cell-based therapeutic research
      iPSC Differentiated Cells Categories

      Neural Stem Cell,
      Neurons & Astrocytes

      Our Ready-to-us, fully characterized Neural Stem Cells (NSCs) at low passage and neural lineage progenitors are derived from control human iPSCs.

      Neural Stem Cell,
      Neurons & Astrocytes

      iPSC Differentiated
      Cardiomyocytes

      Ready-to-use, beating iPSC-differentiated human cardiomyocytes differentiated are ideal for functional assessments in cardiac safety drug screening.

      iPSC Differentiated
      Cardiomyocytes

    • Genome Edited iPSC Lines


      Applied StemCell, a leader in stem cell genome editing has engineered isogenic panels of human iPSCs for hard-to-model or hard-to-source neurological diseases. These extensively characterized, genome edited human iPSC lines have been shown to recapitulate abnormal neuronal phenotypes seen in patient-derived iPSC. 

    • Stem Cell Culture: MEF cells, FBS


      Need high-quality reagents and media to maintain robust and undifferentiated stem cells? Applied StemCell has extensively tested and validated their stem cell culture product line for maintenance of iPSCs and ESCs for robust growth, healthy stem cell morphology, and promotion of pluripotency. Try our stem cell-validated products: treated and untreated mouse embryonic fibroblasts (MEF) cells, FBS.

      Stem Cell Culture: MEF Cells, FBS Categories

      MEF Feeder Cells

      We manufacture CF-1, DR4, Neo-resistant, and SNL 76/7 (STO) MEF feeder cells as untreated cells or treated cells for direct use as a feeder layer.

      MEF Feeder Cells

      ESC-Grade FBS

      ESC-Sure™ Fetal Bovine Serum (PBS) is pre-validated for iPSC/ ESC culture to ensure robust cell growth and promotion of pluripotency.

      Stem Cell Grade FBS

    • iPSC Generation Kit


      Tailor-make your own iPSCs using Applied StemCell’s Human iPSC Reprogramming Kits:

      (1) EZ-iPSC Generation Kit (Retrovirus Vector) (ASK-3012) – Generate pluripotent cell lines from a broad range of tissues and cell types using our well- characterized high efficiency protocol with our specially optimized VSV-G retroviral cocktail.

      (2) EZ-iPSC Generation Kit (Episomal Vector) (ASK-3013) – For integration-free and footprint-free pluripotent cells, try our high efficiency reprogramming kit which uses our proprietary oriP/EBNA-1 episomal vector. The episomal kit does not need small molecules but gives you the flexibility to use feeder-free and feeder-based media.

      Create your own iPS cells, to suit your specific needs, at all stages of your pluripotent stem cell research.

  • TARGATT™ Genome Editing

    • TARGATT™ Knock-in Master Cell Line and Knock-in Kit (HEK293, CHO, and iPSC)


      The TARGATT™ Master Cell Lines (HEK293, CHO, and iPSC) and transgene knock-in kits are an efficient way to generate stable, isogenic, knock-in cell lines and build large mammalian cell libraries, even large transgenes.

      • High knock-in efficiency: with enrichment (up to 90%) or without (up to 40%)
      • Site-specific: well-defined, active, intergenic safe harbor locus
      • Single copy integration
      • Uniform, high level gene expression
      • Unidirectional integration
      • Overcomes challenges posed by random integration

      The TARGATT™ Master Cell Lines are ideal for research in immunocompatible cell therapy & directed-differentiation (iPSCs); gene regulatory elements, protein evolution, antibody/ recombinant protein expression and high-yield bioproduction studies (HEK293 & CHO cells).

    • TARGATT™ Mouse Model Generation


      ASC’s proprietary TARGATT™ transgenic mouse model generation products offer an affordable do-it-yourself option to generate site-specific transgenic mice very efficiently and in as little as 3 months, in your own animal facility. You can purchase all required components to generate your transgenic "knock-in" mice right here from our ASC website:TARGATT™ Plasmids, TARGATT™ attP mice, and TARGATT™ Transgenic Kits for site-specific knock-in of your transgene, and genotyping kits. The TARGATT™ products are designed based on our expertise in TARGATT™ Custom Mouse Model Generation Service and manufactured in our ISO:9001 QMS-certified facility.

      Transgenic core facilities currently using the TARGATT™ system, include UCSF, NIH, MaxPlank, Harvard, Colombia and many more!

      TARGATT™ Mouse Model Generation Categories

      TARGATT™ Plasmids

      Our range of plasmids include various promoters and marker genes to clone your gene of interest for generating your TARGATT™ transgenic mouse models.

      TARGATT™ Plasmids

      TARGATT™ attP Mice

      TARGATT™ attP mice available at Applied StemCell, along with associated kits enable you to make site-specific transgenic mouse models in your lab.

      TARGATT™ attP Mice

      TARGATT™ Transgenic Kits

      The TARGATT™ Transgenic Kit contains the integrase mRNA and buffers to deliver the gene of interest into TARGATT™ attP mice.

      TARGATT™ Transgenic Kits

      TARGATT™ Genotyping Kit

      The TARGATT™ Mouse Genotyping Kit allows for convenient genotyping of your TARGATT™ transgenic knock-in mice via tail biopsy.

      TARGATT™ Genotyping Kit

    • TARGATT™ Cre-Rat


      These transgenic Cre-rat models enable the development of physiologically relevant human cardiovascular or neurological diseases using either ASC's proprietary integrase-based TARGATT™ System or CRISPR/cas9 genome editing technology.   

      These products were supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R44GM108071. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health

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  • Genetically Modified Cell Lines / Isogenic Cell Lines

    • Neuronal Disease Specific Isogenic Knockout iPSCs


      Applied StemCell has engineered isogenic panels of extensively characterized, genome edited human iPSC lines that have been shown to recapitulate abnormal neuronal phenotypes seen in patient-derived iPSC.

      • Engineered iPSCs with knockout (KO) of genes associated with neurological disorders

      • Isogenic panels of iPSCs from a well-characterized control/ master iPSC line

      • Fully characterized KO iPSCs: pluripotency, differentiation, and functional phenotypes of differentiated neurons

      • Overcomes problems with tissue sourcing from diseased patients and varied genetic background which makes results hard to interpret

      Our engineered hiPSC lines are physiologically relevant cell lines to model Parkinson’s disease (PD), Alzheimer’s disease, ALS, Schizophrenia, and Autism. 

    • Reporter iPSC Lines (Isogenic)


      Applied StemCell has engineered two isogenic panels of reporter gene iPSC lines from a fully characterized parental control/ master line (ASE-9109). These reporter lines are ideal for quantitative investigation of intricate genetic and molecular mechanisms underlying lineage-specific development, disease pathology and drug interactions.

      • Two Isogenic panels of “knock-in” (KI) reporter iPSCs engineered from fully characterized parental control iPSCs:

        1. Neuronal Lineage Specific Reporter iPSCs

        2. Safe Harbor Locus Reporter (Master) iPSCs

      • Fully characterized reporter iPSCs: pluripotency, differentiation capability, and functionality

      • Demonstrated use of these reporter lines for high throughput neurotoxicity screening of drug candidates

      For custom reporter line generation, please contact us.

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!