Applied StemCell Products

Applied StemCell Inc. (ASC) offers a large array of products for site specific transgenics, cell culture, and stem cell research. Our products encompass a wide spectrum, from: high quality transgenic kits for knock-in mice and knock-out mice generation, mouse embryonic fibroblast (MEF cells), feeder cells, growth factors, options for stem cell characterization and stem cell differentiation, as well an array of iPSC cells. See below for our full product list.

  • Stem Cell

    • iPSC Cells

      Applied StemCell’s iPSC catalog includes well-characterized, human iPS cell lines suitable for iPS cell culture applications such as genome editing for disease modeling, for differentiation into cellular lineages of choice and drug toxicity/ efficacy screening:

      • Footprint-free iPSC cells (episomal and other integration-free methods) and retroviral-reprogrammed iPSCs
      • Characterized for pluripotency markers, karyotyping and differentiation potential; more characterization options are available
      • Multiple donor tissues: PBMCs, fibroblasts, cord blood, adipocytes from healthy and diseased patients
      • Tested for CRISPR genome editing and differentiation potential into various cell lineages
      • Ideal as master iPSC cell lines to build engineered and/or panels of differentiated isogenic cell lineages
      iPSC & ESC Lines Categories

      iPSC Lines, Human, Normal

      High quality human iPSC lines (hiPSCs) derived from male and female cord blood cells, fibroblasts, and PBMCs, for genome editing and differentiation.

      iPSC Lines, Human, Normal

      iPSC Cells, Human, Disease

      Patient iPSC lines derived from various somatic cells of patients with Parkinson’s disease and Diabetes (II).

      iPSC Cells, Human, Disease

    • iPSC Differentiated Cells

      We offer fully characterized progenitors and differentiated cell lines derived from integration-free iPSCs using feeder-free culture protocols: Neural stem cells (NSCs), Neurons (Dopaminergic and Cortical neurons), Astrocytes, and Cardiomyocytes.

      The cell lines have been characterized for the expression of characteristic biomarkers using immunohistochemistry and for functional viability. Our isogenic panel of neural lineage cells also have been validated by whole genome profiling to ensure cellular integrity and quality.

      These cell lines are ideal for various applications in biomedical and applied research:

      • Disease Modeling
      • Drug target discovery
      • Neurotoxicity and cardiotoxicity drug screening
      • Drug efficacy testing for new neurological drug candidates
      • Cell-based therapeutic research
      iPSC Differentiated Cells Categories

      Neural Stem Cell,
      Neurons & Astrocytes

      Our Ready-to-us, fully characterized Neural Stem Cells (NSCs) at low passage and neural lineage progenitors are derived from control human iPSCs.

      Neural Stem Cell,
      Neurons & Astrocytes

      iPSC Differentiated

      Ready-to-use, beating iPSC-differentiated human cardiomyocytes differentiated are ideal for functional assessments in cardiac safety drug screening.

      iPSC Differentiated

    • Genome Edited iPSC Lines

      Applied StemCell, a leader in stem cell genome editing has engineered isogenic panels of human iPSCs for hard-to-model or hard-to-source neurological diseases. These extensively characterized, genome edited human iPSC lines have been shown to recapitulate abnormal neuronal phenotypes seen in patient-derived iPSC. 

    • Stem Cell Culture: MEF cells, FBS

      Need high-quality reagents and media to maintain robust and undifferentiated stem cells? Applied StemCell has extensively tested and validated their stem cell culture product line for maintenance of iPSCs and ESCs for robust growth, healthy stem cell morphology, and promotion of pluripotency. Try our stem cell-validated products: treated and untreated mouse embryonic fibroblasts (MEF) cells, FBS.

      Stem Cell Culture: MEF Cells, FBS Categories

      MEF Feeder Cells

      We manufacture CF-1, DR4, and Neo-resistant MEF feeder cells as untreated cells or treated cells for direct use as a feeder layer.

      MEF Feeder Cells

      ESC-Grade FBS

      ESC-Sure™ Fetal Bovine Serum (PBS) is pre-validated for iPSC/ ESC culture to ensure robust cell growth and promotion of pluripotency.

      Stem Cell Grade FBS

    • iPSC Generation Kit

      Tailor-make your own iPSCs using Applied StemCell’s Human iPSC Reprogramming Kits:

      (1) EZ-iPSC Generation Kit (Retrovirus Vector) (ASK-3012) – Generate pluripotent cell lines from a broad range of tissues and cell types using our well- characterized high efficiency protocol with our specially optimized VSV-G retroviral cocktail.

      (2) EZ-iPSC Generation Kit (Episomal Vector) (ASK-3013) – For integration-free and footprint-free pluripotent cells, try our high efficiency reprogramming kit which uses our proprietary oriP/EBNA-1 episomal vector. The episomal kit does not need small molecules but gives you the flexibility to use feeder-free and feeder-based media.

      Create your own iPS cells, to suit your specific needs, at all stages of your pluripotent stem cell research.

  • TARGATT™ Genome Editing

    • TARGATT™ Mouse Model Generation

      ASC’s proprietary TARGATT™ transgenic mouse model generation products offer an affordable do-it-yourself option to generate site-specific transgenic mice very efficiently and in as little as 3 months, in your own animal facility. You can purchase all required components to generate your transgenic "knock-in" mice right here from our ASC website:TARGATT™ Plasmids, TARGATT™ attP mice, and TARGATT™ Transgenic Kits for site-specific knock-in of your transgene, and genotyping kits. The TARGATT™ products are designed based on our expertise in TARGATT™ Custom Mouse Model Generation Service and manufactured in our ISO:9001 QMS-certified facility.

      Transgenic core facilities currently using the TARGATT™ system, include UCSF, NIH, MaxPlank, Harvard, Colombia and many more!

      TARGATT™ Mouse Model Generation Categories

      TARGATT™ Plasmids

      Our range of plasmids include various promoters and marker genes to clone your gene of interest for generating your TARGATT™ transgenic mouse models.

      TARGATT™ Plasmids

      TARGATT™ attP Mice

      TARGATT™ attP mice available at Applied StemCell, along with associated kits enable you to make site-specific transgenic mouse models in your lab.

      TARGATT™ attP Mice

      TARGATT™ Transgenic Kits

      The TARGATT™ Transgenic Kit contains the integrase mRNA and buffers to deliver the gene of interest into TARGATT™ attP mice.

      TARGATT™ Transgenic Kits

      TARGATT™ Genotyping Kit

      The TARGATT™ Mouse Genotyping Kit allows for convenient genotyping of your TARGATT™ transgenic knock-in mice via tail biopsy.

      TARGATT™ Genotyping Kit

    • TARGATT™ Knock-in Master Cell Line

      Use our TARGATT™ Master Cell Lines (HEK293, CHO, and iPSC) and kits to generate site-specific knock-in cell lines efficiently (even large transgene). TARGATT™’s safe harbor locus-based gene integration overcomes challenges associated with random integration methods.

      • Higher knock-in efficiency compared to other knock-in technologies, with or without clonal selection
      • Single copy integration is compatible with gene amplification
      • Uniform, high level gene expression
      • Fast and simple gene knock-in protocol

      The TARGATT™ Master Cell Lines are ideal for large isogenic cell library construction for antibody and recombinant protein expression and high-yield bioproduction studies.

  • Genetically Modified Cell Lines / Isogenic Cell Lines

    • Neuronal Disease Specific Isogenic Knockout iPSCs

      Applied StemCell has engineered isogenic panels of extensively characterized, genome edited human iPSC lines that have been shown to recapitulate abnormal neuronal phenotypes seen in patient-derived iPSC.

      • Engineered iPSCs with knockout (KO) of genes associated with neurological disorders

      • Isogenic panels of iPSCs from a well-characterized control/ master iPSC line

      • Fully characterized KO iPSCs: pluripotency, differentiation, and functional phenotypes of differentiated neurons

      • Overcomes problems with tissue sourcing from diseased patients and varied genetic background which makes results hard to interpret

      Our engineered hiPSC lines are physiologically relevant cell lines to model Parkinson’s disease (PD), Alzheimer’s disease, ALS, Schizophrenia, and Autism. 

    • Reporter iPSC Lines (Isogenic)

      Applied StemCell has engineered two isogenic panels of reporter gene iPSC lines from a fully characterized parental control/ master line (ASE-9109). These reporter lines are ideal for quantitative investigation of intricate genetic and molecular mechanisms underlying lineage-specific development, disease pathology and drug interactions.

      • Two Isogenic panels of “knock-in” (KI) reporter iPSCs engineered from fully characterized parental control iPSCs:

        1. Neuronal Lineage Specific Reporter iPSCs

        2. Safe Harbor Locus Reporter (Master) iPSCs

      • Fully characterized reporter iPSCs: pluripotency, differentiation capability, and functionality

      • Demonstrated use of these reporter lines for high throughput neurotoxicity screening of drug candidates

      For custom reporter line generation, please contact us.

    • Isogenic Lines

      Isogenic panel of clinically relevant cancer mutation cell lines! Using our expertise garnered as a leading CRISPR service provider, ASC has engineered 250+ isogenic, CRISPR cell lines with cancer mutations based on the COSMIC database, for use as clinically relevant in vitro (cell line) models for cancer research, drug discovery and screening applications.

      • 271 Isogenic cell lines with largest mutation panel in market
      • 40+ clinically actionable genes in MAPK Pathway and more (Ex. EGFR, ALK,
      • Isogenically paired wild-type and mutant cell lines
      • Footprint-free genome editing using CRISPR/Cas9; no genomic scars
      • Knockout and knock-in based on COSMIC database
      • Mutation are: clonally homogeneous, genetically validated and 100% homozygous
  • Cancer Research Tools

    • Substrate-free Autobioluminescent Cell Lines and Vectors

      Applied StemCell has developed a genetically encoded synthetic luciferase bioluminescence system that encodes all of the components required for signal initiation and maintenance. This allows cells to continuously and autonomously produce a bioluminescent signal without the need for additional chemical or fluorescent substrate or stimulation, and without sample destruction.

      • Decreased costs – no need to purchase luciferin

      • Increased imaging flexibility – image any time

      • Easy integration into automated systems – no treatment required for light production

      • Image the same samples repeatedly without cellular destruction

      • Reduced hands on time – simply plate and image

      • Non-invasive in vivo tumor tracking in small animal models - image directly through tissue

  • Sample Prep

    • Human Fibroblast Immortalization Kit

      Primary cells undergo a finite number of cell divisions in culture, after which they enter a state of senescence and no longer divide. To circumvent replicative senescence, the Simian virus 40 (SV40) T antigen is introduced to primary cells. The Simian virus 40 (SV40) is used as a reliable agent for the immortalization of different cell types and the mechanism of the SV40 T antigen in cell immortalization is well understood. Recent studies have also shown that SV40 T antigen can induce Telomerase activity in the infected cells.

      Applied StemCell’s Human Fibroblast Immortalization Kits include an Immortalization Reagent (packaged lentivector constructs provided as frozen VSV-G pseudotyped viral particles) and a Virus Transduction Enhancer which can increase transduction rate up to 10-fold.

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!