Applied StemCell Products

Applied StemCell Inc. (ASC) offers a large array of products for site specific transgenics, cell culture, and stem cell research. Our products encompass a wide spectrum, from: high quality transgenic kits for knock-in mice and knock-out mice generation, mouse embryonic fibroblast (MEF cells), feeder cells, growth factors, options for stem cell characterization and stem cell differentiation, as well an array of iPSC cells. See below for our full product list.

  • Stem Cell

    • Induced Pluripotent Stem Cells (iPSCs)

      iPSC Products

      Applied StemCell’s induced pluripotent stem cell (iPSC) catalog includes well-characterized, human iPS cell lines suitable for iPSC culture applications. The high-quality iPSCs are ideal for genome editing, differentiation, and drug toxicity/efficacy screening projects:

      • Footprint-free iPSC cells (episomal and other integration-free methods) and retroviral-reprogrammed iPSCs
      • Characterized for pluripotency markers, karyotyping, and differentiation potential; more characterization options are available
      • Multiple donor tissues: PBMCs, fibroblasts, cord blood, adipocytes from healthy and diseased patients
      • Tested for CRISPR genome editing and differentiation potential into various cell lineages
      • Ideal as master iPSC cell lines to build engineered and/or panels of differentiated isogenic cell lineages

      Along with human iPSCs, we provide mouse embryonic stem cells (mESCs) and mouse iPSCs. Contact us today to learn more about our ready-to-use iPSC control lines.

      iPSCs & mESCs

      Normal, Human Induced Pluripotent Stem Cells (hiPSCs)

      Normal Human iPSCs

      High-quality human iPSC lines(hiPSCs) derived from male and female cord blood cells, fibroblasts, and PBMCs for genome editing and differentiation.

      Normal Human iPSCs

      GMP Grade iPSC Services & Products

      GMP Garde iPSC Services & Products

      New GMP Grade iPSC services are now available. Contact us today to learn more.

      GMP Grade iPSC Services & Products

      Engineered Induced Pluripotent Stem Cells (iPSCs)

      Genome Edited iPSCs

      Well-characterized, engineered iPSCs for in vitro models of several neurodegenerative diseases, including Parkinson’s Disease, Alzheimer’s Disease, ALS, Autism, and more.

      Genome Edited iPSCs

      Non-human Stem Cells & iPSCs; mESCs

      Non-human Stem Cells & iPSCs

      Mouse induced pluripotent stem cells and mouse embryonic stem cells.

      Non-human Stem Cells & iPSCs

    • iPSC Differentiated Cells

      We offer fully characterized progenitors and differentiated cell lines derived from integration-free iPSCs using feeder-free culture protocols: Neural stem cells (NSCs), Neurons (Dopaminergic and Cortical neurons), Astrocytes, Microglia, and Cardiomyocytes. The cell lines have been characterized for the expression of characteristic biomarkers using immunohistochemistry and for functional viability. 

      Ideal for various applications in biomedical and applied research:

      • Disease Modeling
      • Drug target discovery
      • Neurotoxicity and cardiotoxicity drug screening
      • Drug efficacy testing for new neurological drug candidates
      • Cell-based therapeutic research
    • Genome Edited iPSC Lines

      Applied StemCell offers research-ready, isogenic panel of genome edited human iPSCs and their differentiated cells from well-characterized control, parental iPSCs. These gene edited human iPSCs and differentiated neuronal lineage cell lines are ideal for hard-to-model or hard-to-source neurological diseases disease modeling, neurotoxicity screening, and drug discovery and screening studies. 

    • Stem Cell Culture: MEF cells

      Need high-quality reagents and media to maintain robust and undifferentiated stem cells? Applied StemCell has extensively tested and validated their stem cell culture product line for maintenance of iPSCs and ESCs for robust growth, healthy stem cell morphology, and promotion of pluripotency. Try our stem cell-validated treated and untreated mouse embryonic fibroblasts (MEF) cells.

      Stem Cell Culture: MEF Cells

      MEF Feeder Cells

      We manufacture CF-1, DR4, Neo-resistant, and SNL 76/7 (STO) MEF feeder cells as untreated cells or treated cells for direct use as a feeder layer.

      MEF Feeder Cells

    • iPSC Generation Kit

      Tailor-make your own iPSCs using Applied StemCell’s Human iPSC Reprogramming Kits:

      (1) EZ-iPSC Generation Kit (Retrovirus Vector) (ASK-3012) – Generate pluripotent cell lines from a broad range of tissues and cell types using our well- characterized high efficiency protocol with our specially optimized VSV-G retroviral cocktail.

      (2) EZ-iPSC Generation Kit (Episomal Vector) (ASK-3013) – For integration-free and footprint-free pluripotent cells, try our high efficiency reprogramming kit which uses our proprietary oriP/EBNA-1 episomal vector. The episomal kit does not need small molecules but gives you the flexibility to use feeder-free and feeder-based media.

      Create your own iPS cells, to suit your specific needs, at all stages of your pluripotent stem cell research.

    • iPSC 3D Culture

      Applied StemCell (ASC) now provides the tools you need for in vitro 3D induced pluripotent stem cell (iPSC) cultures. The MyEZGel™ iPSC Matrix kit allows the formation of a 3D microenvironment for iPSC growth. With the MyEZGel™ kit, your cells do not have to undergo acidic or chill conditions, and the cells can be easily harvested from the matrix.

      ASC also offers a 3D gel of our iPSC control line, ASE-9211. Our ASE-9211 “Master” iPSC line is being used as a benchmark material for developing measurements and standards for the rapidly evolving genetically engineered cells used in cell therapy research. Contact us today to learn more!


  • TARGATT™ Genome Editing

    • TARGATT™ Knock-in Master Cell Line and Knock-in Kit (HEK293, CHO, and iPSC)

      The TARGATT™ Master Cell Lines (HEK293, CHO, and iPSC) and transgene knock-in kits are an efficient way to generate stable, isogenic, knock-in cell lines and build large mammalian cell libraries, even large transgenes.

      • High knock-in efficiency: with enrichment (up to 90%) or without (up to 40%)
      • Site-specific: well-defined, active, intergenic safe harbor locus
      • Single copy integration
      • Uniform, high level gene expression
      • Unidirectional integration
      • Overcomes challenges posed by random integration

      The TARGATT™ Master Cell Lines are ideal for research in immunocompatible cell therapy & directed-differentiation (iPSCs); gene regulatory elements, protein evolution, antibody/ recombinant protein expression and high-yield bioproduction studies (HEK293 & CHO cells).

    • TARGATT™ Mouse Model Generation

      ASC’s proprietary TARGATT™ transgenic mouse model generation products offer an affordable do-it-yourself option to generate site-specific transgenic mice very efficiently and in as little as 3 months, in your own animal facility. You can purchase all required components to generate your transgenic "knock-in" mice right here from our ASC website:TARGATT™ Plasmids, TARGATT™ attP mice, and TARGATT™ Transgenic Kits for site-specific knock-in of your transgene, and genotyping kits. The TARGATT™ products are designed based on our expertise in TARGATT™ Custom Mouse Model Generation Service and manufactured in our ISO:9001 QMS-certified facility.

      Transgenic core facilities currently using the TARGATT™ system, include UCSF, NIH, MaxPlank, Harvard, Colombia and many more!

      TARGATT™ Mouse Model Generation Categories

      TARGATT™ Plasmids

      Our range of plasmids include various promoters and marker genes to clone your gene of interest for generating your TARGATT™ transgenic mouse models.

      TARGATT™ Plasmids

      TARGATT™ attP Mice

      TARGATT™ attP mice available at Applied StemCell, along with associated kits enable you to make site-specific transgenic mouse models in your lab.

      TARGATT™ attP Mice

      TARGATT™ Transgenic Kits

      The TARGATT™ Transgenic Kit contains the integrase mRNA and buffers to deliver the gene of interest into TARGATT™ attP mice.

      TARGATT™ Transgenic Kits

      TARGATT™ Genotyping Kit

      The TARGATT™ Mouse Genotyping Kit allows for convenient genotyping of your TARGATT™ transgenic knock-in mice via tail biopsy.

      TARGATT™ Genotyping Kit

    • TARGATT™ Cre-Rat

      These transgenic Cre rat models have been developed using either ASC's proprietary integrase-based TARGATT™ System or CRISPR/Cas9 genome editing technology. These Cre rat lines can enable the development of physiologically relevant human cardiovascular, neurological diseases and other disease specific models.

      These products were supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R44GM108071. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

      Applied StemCell, Inc. (ASC) announces that as of July 1, 2020, customers can order the Cre rat lines for research and industrial applications, through our partnering with the Rat Resource & Research Center (RRRC). For more questions regarding the Cre rat models, please visit the RRRC website at or contact the RRRC customer service email at

      These strains will be available as:



      Strain Name





















  • Genetically Modified Cell Lines / Isogenic Cell Lines

    • Neuronal Disease Specific Isogenic Knockout iPSCs

      Applied StemCell has engineered isogenic panels of extensively characterized, genome edited human iPSC lines that have been shown to recapitulate abnormal neuronal phenotypes seen in patient-derived iPSC.

      • Engineered iPSCs with knockout (KO) of genes associated with neurological disorders

      • Isogenic panels of iPSCs from a well-characterized control/ master iPSC line

      • Fully characterized KO iPSCs: pluripotency, differentiation, and functional phenotypes of differentiated neurons

      • Overcomes problems with tissue sourcing from diseased patients and varied genetic background which makes results hard to interpret

      Our engineered hiPSC lines are physiologically relevant cell lines to model Parkinson’s disease (PD), Alzheimer’s disease, ALS, Schizophrenia, and Autism. 

      Neuronal Disease-Specific Isogenic Knockout Lines: 

      Isogenic Knock-out Lines

      Associated Disease


      PARK2 -/-


      Biallelic KO

      PARK7 -/-


      Biallelic KO

      PINK1 -/-


      Biallelic KO

      LRRK2 -/-


      Biallelic KO

      Park2-/-; Park7-/-


      Biallelic KO

      Park2-/-; Pink1-/-


      Biallelic KO

      APOE -/-

      Alzheimer's disease

      Biallelic KO

      SOD1 -/-


      Biallelic KO

      DISC1 -/-


      Biallelic KO

      CNTNAP2 -/-


      Biallelic KO

      BDNF -/-


      Biallelic KO


    • Reporter iPSC Lines (Isogenic)

      Applied StemCell has engineered two isogenic panels of reporter gene iPSC lines from a fully characterized parental control/ master line (ASE-9109). These reporter lines are ideal for quantitative investigation of intricate genetic and molecular mechanisms underlying lineage-specific development, disease pathology and drug interactions.

      • Two Isogenic panels of “knock-in” (KI) reporter iPSCs engineered from fully characterized parental control iPSCs:

        1. Neuronal Lineage Specific Reporter iPSCs

        2. Safe Harbor Locus Reporter (Master) iPSCs

      • Fully characterized reporter iPSCs: pluripotency, differentiation capability, and functionality

      • Demonstrated use of these reporter lines for high throughput neurotoxicity screening of drug candidates

      For custom reporter line generation, please contact us.

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!