Products

Applied StemCell’s iPSC catalog includes a broad range of human iPS cell lines suitable for iPS cell culture applications such as disease modeling, differentiation into cellular lineages of choice and drug toxicity/ efficacy screening:

• Footprint-free (episomal and other methods) and retroviral iPSC lines
• Multiple donor tissues: PBMCs, fibroblasts, cord blood, adipocytes from healthy and diseased patients
• Also available, pluripotent stem cells from other species: mouse, pig, guinea pigs reprogrammed using episomal or retroviral reprogramming methods

We offer fully characterized progenitors and differentiated cell lines derived from integration-free iPSCs using feeder-free culture protocols: Neural stem cells (NSCs), Neurons (Dopaminergic and Cortical neurons), Astrocytes, and Cardiomyocytes.

The cell lines have been characterized for the expression of characteristic biomarkers using immunohistochemistry and for functional viability. Our isogenic panel of neural lineage cells also have been validated by whole genome profiling to ensure cellular integrity and quality.

These cell lines are ideal for various applications in biomedical and applied research:

• Disease Modeling
• Drug target discovery
• Neurotoxicity and cardiotoxicity drug screening
• Drug efficacy testing for new neurological drug candidates
• Cell-based therapeutic research

Applied StemCell, a leader in stem cell genome editing has engineered isogenic panels of human iPSCs for hard-to-model or hard-to-source neurological diseases. These extensively characterized, genome edited human iPSC lines have been shown to recapitulate abnormal neuronal phenotypes seen in patient-derived iPSC. 

• Isogenic genome edited iPSCs engineered from fully characterized control iPSC lines

• The genome edited iPSCs are characterized for pluripotency, differentiation capability, and functionality; and provide a fully encompassing variety of tissue models for various applications

• Three panels of isogenic genome-edited iPSC lines:

1. Neuronal disease-specific gene knockout lines

2. Lineage-specific reporter gene knock-in lines

3. Safe harbor locus reporter gene knock-in lines

Need high-quality reagents and media to maintain robust and undifferentiated stem cells? Applied StemCell has extensively tested and validated their stem cell culture product line for maintenance of iPSCs and ESCs for robust growth, healthy stem cell morphology, and promotion of pluripotency. Try our stem cell-validated products: treated and untreated mouse embryonic fibroblasts (MEF) cells, FBS, 3D matrix, serum- and feeder-free (SFFM) media, and cell culture supplements.

Tailor-make your own iPSCs using Applied StemCell’s Human iPSC Reprogramming Kits:

(1) EZ-iPSC Generation Kit (Retrovirus Vector) (ASK-3012) – Generate pluripotent cell lines from a broad range of tissues and cell types using our well- characterized high efficiency protocol with our specially optimized VSV-G retroviral cocktail.

(2) EZ-iPSC Generation Kit (Episomal Vector) (ASK-3013) – For integration-free and footprint-free pluripotent cells, try our high efficiency reprogramming kit which uses our proprietary oriP/EBNA-1 episomal vector. The episomal kit does not need small molecules but gives you the flexibility to use feeder-free and feeder-based media.

Create your own iPS cells, to suit your specific needs, at all stages of your pluripotent stem cell research.

Applied StemCell’s CRISPRCLEAR™ kits for cell line model generation are do-it-yourself kits for affordable and efficient cell line model generation. These kits are transfection-ready and are designed with our extensive expertise garnered from successfully completing >1000 CRISPR projects and offer an efficient alternative to generate your own cell line models. We use our proprietary designing strategy and gRNA validation techniques to ensure highly efficient gene modification in your cell line. The kits are manufactured using optimized protocols in our ISO:9001 QMS-certified US facility. A variety of kits are available for gene knockout, point mutation and knock-in models in human and mouse cell lines.

ASC’s proprietary TARGATT™ transgenic mouse model generation products offer an affordable do-it-yourself option to generate site-specific transgenic mice very efficiently and in as little as 3 months, in your own animal facility. You can purchase all required components to generate your transgenic "knock-in" mice right here from our ASC website: TARGATT™ attP mice, TARGATT™ plasmids, transgenic kits for site-specific knock-in of your transgene, and genotyping kits. The TARGATT™ products are designed based on our expertise in TARGATT™ Custom Mouse Model Generation Service and manufactured in our ISO:9001 QMS-certified facility.

Transgenic core facilities currently using the TARGATT™ system, include UCSF, NIH, MaxPlank, Harvard, Colombia and many more!

Use our TARGATT™ Master Cell Lines (HEK29T or CHO) and kits to generate site-specific knock-in cell lines efficiently (even large transgene). TARGATT™’s safe harbor locus-based gene integration overcomes challenges associated with random integration methods.

1. Higher knock-in efficiency compared to other gene integration technologies, with or without clonal selection

2. Fast and simple gene knock-in protocol

3. Single copy integration is compatible with gene amplification

4. No integration of bacterial backbone which might reduce gene expression

5. Uniform, high level gene expression

The TARGATT™ Master Cell Lines are ideal for large isogenic cell library construction and high-yield bioproduction studies.

Also available,  TARGATT™-mESC Master Cell Line and Custom TARGATT™ Site-Specific Knock-in Cell Line Service.

Applied StemCell has engineered isogenic panels of extensively characterized, genome edited human iPSC lines that have been shown to recapitulate abnormal neuronal phenotypes seen in patient-derived iPSC.

• Engineered iPSCs with knockout (KO) of genes associated with neurological disorders

• Isogenic panels of iPSCs from a well-characterized control/ master iPSC line

• Fully characterized KO iPSCs: pluripotency, differentiation, and functional phenotypes of differentiated neurons

• Overcomes problems with tissue sourcing from diseased patients and varied genetic background which makes results hard to interpret

Our engineered hiPSC lines are physiologically relevant cell lines to model Parkinson’s disease (PD), Alzheimer’s disease, ALS, Schizophrenia, and Autism. 

Applied StemCell has engineered two isogenic panels of reporter gene iPSC lines from a fully characterized parental control/ master line (ASE-9109). These reporter lines are ideal for quantitative investigation of intricate genetic and molecular mechanisms underlying lineage-specific development, disease pathology and drug interactions.

• Two Isogenic panels of “knock-in” (KI) reporter iPSCs engineered from fully characterized parental control iPSCs:

1. Neuronal Lineage Specific Reporter iPSCs

2. Safe Harbor Locus Reporter (Master) iPSCs

• Fully characterized reporter iPSCs: pluripotency, differentiation capability, and functionality

• Demonstrated use of these reporter lines for high throughput neurotoxicity screening of drug candidates

For custom reporter line generation, please contact us.

Applied StemCell offers a reliable and efficient alternative to traditional CRISPR/Cas9 protocols with our Cas9-Expressing Cell Lines. These cell lines have an integrated human codon optimized Cas9 that stably expresses Cas9 for DNA cleavage, for a smoother workflow with fewer transfections and subsequently a higher cell viability. Generate your own isogenic knockout, gene knock-in and gene tagging cell line models for consistent, reproducible results that are ideal for antibody validation, high throughput drug screening, and gRNA validation.

We have ready-to-use Cas9 expressing cells in some popular human and murine cell line models:

• Jurkat-Cas9
• K562-Cas9
• HEK293-Cas9
• mESC (mouse embryonic cell)-Cas9

Inquire for custom Cas9 expressing cell line model service in a cell line of your interest.

Applied StemCell has developed a genetically encoded synthetic luciferase bioluminescence system that encodes all of the components required for signal initiation and maintenance. This allows cells to continuously and autonomously produce a bioluminescent signal without the need for additional chemical or fluorescent substrate or stimulation, and without sample destruction.

• Decreased costs – no need to purchase luciferin

• Increased imaging flexibility – image any time

• Easy integration into automated systems – no treatment required for light production

• Image the same samples repeatedly without cellular destruction

• Reduced hands on time – simply plate and image

• Non-invasive in vivo tumor tracking in small animal models - image directly through tissue

MyEZ Gel™ CDX/PDX is a protein based solubilized base membrane synthesized from commonly used amino acids, and minerals. MyEZ Gel™ CDX/PDX is a powerful reagent to package and deliver cancer cells, primary cancer cells, or stem cells in vivo for xenograft animal models.

Advantages of MyEZ Gel™ CDX/PDX:

• No more icing: All operation and growth procedures are done at room temperature or 37°C in neutral pH.
• Easy procedure: cells are easily encapsulated and injected into a targeted location in vivo. Cells can grow out of the matrix to form a tumor and the matrix is degraded into amino acids and absorbed.
• Well-defined system: Gel matrix has defined components.
• Biocompatible: Gel matrix is highly biocompatible with biological environments and accurately reflects a cell microenvironment.

Primary cells undergo a finite number of cell divisions in culture, after which they enter a state of senescence and no longer divide. To circumvent replicative senescence, the Simian virus 40 (SV40) T antigen is introduced to primary cells. SV40 has been shown to be the most reliable agent for the immortalization of different cell types and the mechanism of the SV40 T antigen in cell immortalization is relatively well understood.

Applied StemCell’s Human Immortalized Fibroblasts (SV40) are derived from healthy skin tissue and retain the characteristics of the primary cells they came from. Our Human Immortalized Fibroblasts serve as a continuous source of cells that eliminates batch to batch variability, provides an avenue for experimental reproducibility, and are easy to maintain.

ASC’s PCR Mycoplasma Detection Kit identifies a conserved rRNA coding region in the mycoplasma genome, enabling it to detect majority of the mycoplasma species. This all-inclusive PCR Kit is designed for routine monitoring of mycoplasma-free cell lines and can be used in conjunction with a secondary detection method such as cell culture isolation, ELISA, etc.

• Highly sensitive PCR method to detect mycoplasma

• > 95% of commonly occurring mycoplasma species contaminating cell lines

• PCR Primer Mix includes an internal control to avoid false negatives and provide reliable results

• Straightforward, easy-to-use protocol enables routine daily testing of cell lines - just use supernatant in PCR reaction

• Significantly short protocol time provides you with results in a couple of hours

Primary cells undergo a finite number of cell divisions in culture, after which they enter a state of senescence and no longer divide. To circumvent replicative senescence, the Simian virus 40 (SV40) T antigen is introduced to primary cells. SV40 has been shown to be the most reliable agent for the immortalization of different cell types and the mechanism of the SV40 T antigen in cell immortalization is relatively well understood. Recent studies have also shown that SV40 T antigen can induce Telomerase activity in the infected cells.

Applied StemCell’s Immortalization Kits include an Immortalization Reagent which is a packaged lentivector constructs provided as frozen VSV-G pseudotyped viral particles and a Virus Transduction Enhancer which can increase transduction rate up to 10 fold.