Products

Applied StemCell Products

Applied StemCell Inc. (ASC) offers a large array of products for site specific transgenics, cell culture, and stem cell research. Our products encompass a wide spectrum, from: high quality transgenic kits for knock-in mice and knock-out mice generation, mouse embryonic fibroblast (MEF cells), feeder cells, growth factors, options for stem cell characterization and stem cell differentiation, as well an array of iPSC cells. See below for our full product list.

  • Stem Cell

    • iPSC & ESC Lines

      Applied StemCell’s iPSC catalog includes a broad range of iPS cell lines: footprint-free (episomal) and retroviral iPSC lines from different tissues (PBMCs, fibroblasts, cord blood, adipocytes) from healthy and diseased patients; iPSCs derived from mouse, pig, guinea pigs using episomal or retroviral reprogramming methods, and various mouse ESC lines that have been tested for successful germline transmission. These cells are ideal for all iPS cell culture applications such as disease modeling, differentiation into cellular lineages of choice and drug toxicity/efficacy screening.

    • iPSC Differentiated Cells

      We offer fully characterized iPSC-differentiated progenitors and differentiated cell lines derived from integration-free iPSCs using feeder-free culture protocols: Neural stem cells (NSCs), Neurons (Dopaminergic and Cortical neurons), Astrocytes, PBMCs and Cardiomyocytes.

    • Gene Edited iPSC Lines

      ASC’s gene edited iPS Cell Line catalog includes isogenic panels of gene edited lines from control iPSC line (ASE-9109) that are fully characterized and are ideal for disease modeling: (1) neuronal disease-specific isogenic knockout line; (2) lineage-specific reporter gene knock-in lines; (3) safe harbor locus reporter gene knock-in iPS lines.

    • iPSC Generation Kit

      Human EZ- iPSC Generation kits - Reprogram your somatic cells to iPSC with the Retroviral or the Episomal kit.

    • Stem Cell Culture System

      Applied StemCell also supports its induced pluripotent stem cell lines (iPSCs) and embryonic stem cells (ES cells, ESCs) pipeline with medium and supplements for optimal and robust stem cell culture. Our catalog includes media for culturing human and mouse iPSC/ ESCs, including ESC- qualified FBS and serum/ feeder-free (SFFM) medium.

    • Stem Cell Characterization

      Stem Cell Marker kits. Characterization of embryonic stem cells and induced pluripotent stem cells by pluripotency protein or RNA marker kits and stem cell gene arrays.

  • CRISPR/Cas9 Genome Editing

    • Cell Line Model Generation

      Applied StemCell Genome Editing

      Applied StemCell offers a large selection of gene-targeting products including TARGATT™ Site-specific Knock-in Cell Generation Kits and CRISPR Genome Editing Kits.

      Genome Editing Technologies Comparison:

        

    • Animal Model Generation

      Applied StemCell CRISPR Services

       In vivo-grade, injection-ready CRISPR kits for the generation of transgenic mice and rats. 

       

  • TARGATT™ Genome Editing

    • TARGATT™ Mouse Model Generation

      Applied StemCell TARGATT™ Products for Site-Specific Knock-in Mouse Generation

      Our proprietary site-specific DNA integration system, TARGATT™ lets you create site-specific transgenic mice in a more efficient and faster way compared to traditional methods. Generating a transgenic mouse (e.g. knock-in mouse) by conventional methods like pronuclear microinjection or lentiviral injection has several limitations, one of them being random insertion of the transgene. Random insertion of a transgene results in position effect where either the transgene is prone to silencing or endogenous gene expression is disrupted. Furthermore, random transgene insertion in the transgenic mouse usually happens in multiple copies, resulting in repeat induced silencing and genomic instability. The TARGATT™ technology uses PhiC31 integrase to insert any gene of interest into a specific docking-site that was pre-engineered into an intergenic and transcriptionally active genomic locus. Applied StemCell can create site-specific knock-in mice for you in as little as 3 months. Using our novel TARGATT™ system, a gene of interest can be inserted at a well-characterized, transcriptionally-active locus in the mouse genome with guaranteed transgene expression. Our scientists at Applied StemCell can create a TARGATT™ mouse for you, or you can purchase the animals and reagents to make your own.

      Fast! Reliable! Advantages of TARGATT™ Technology

      1. High integration efficiency mediated by PhiC31 integrase reduces time and cost.
      2. Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high expression levels of the transgene.
      3. Integration at intergenic region ensures that no internal genes are interrupted.
      4. Single copy gene integration eliminates repeat-induced gene silencing and genomic instability.
      5. Site-specific integration allows a precise comparison of the effects of the transgenes among different lines.

      Ask us for a list of core facilities currently using the TARGATT™ system, including UCSF, NIH, MaxPlank, Harvard, Colombia and many more!

    • TARGATT™ Knockin Master Cell Line

      Rapid generation of site-directed knock-in cell lines.

      Applied StemCell, Inc., offers a large selection of gene-targeting products including TARGATT™ Site-specific Knock-in Cell Line. The cell line kit includes a master cell line with a TARGATT™ docking site (attP) at a transcriptionally active locus (the H11 locus on chromosome 11), and vectors for TARGATT™ integrase and DNA integration. With this method, large DNA fragments can be inserted at the H11 locus at high efficiency with robust transgene expression.

  • Genetically Modified Cell Lines / Isogenic Cell Lines

    • Cas9 Expressing Cell Lines

      Cell Lines expressing the CRISPR Cas9 nuclease enable you to carry out CRISPR genome editing applications including cell based assays and drug discovery.  

       

  • Xenograft Model Research Tools

    • Substrate-free Autobioluminescent Cell Lines and Vectors

      Eliminate time consuming substrate injection with Luciferase. We can quickly modify your cell lines to express an autobioluminescent reporter and return them to your laboratory for in vivo imaging. 4 major cell lines (HCT116, HEK293, MCF7, T47D) engineered to be autonomously bioluminescent. Custom Cell Line Developement: we can also quickly modify your cell lines to express an autobioluminescent reporter and return them to your laboratory for in vivo imaging as well. 

       

    • MyEZ Gel™ CDX/PDX Models Injection Media

      MyEZGel CDX/PDX is a protein based solubilized base membrane synthesized from commonly used amino acids, and minerals.  The amino acids include Arginine, Aspartate, Glycine, Isoleucine, Leucine, Lysine, Phenylalanine, Proline, Serine, and Valine. MyEZGel CDX/PDX is compatible with various adhesion proteins and cell growth factors, including but not limited to laminin, collagens, VE-growth factor, fibroblast growth factor, and other growth factors. MyEZGel CDX/PDX is a powerful reagent to package and deliver cancer cells, primary cancer cells, or stem cells in vivo for xenograft animal models.

  • Primary Cells and Sample Prep