Products

Applied StemCell Products

Applied StemCell Inc. (ASC) offers a large array of products for site specific transgenics, cell culture, and stem cell research. Our products encompass a wide spectrum, from: high quality transgenic kits for knock-in mice and knock-out mice generation, mouse embryonic fibroblast (MEF cells), feeder cells, growth factors, options for stem cell characterization and stem cell differentiation, as well an array of iPSC cells. See below for our full product list.

  • Stem Cell

    • iPSC & ESC Lines

      Applied StemCell’s iPSC catalog includes a broad range of iPS cell lines: footprint-free (episomal) and retroviral iPSC lines from different tissues (PBMCs, fibroblasts, cord blood, adipocytes) from healthy and diseased patients; iPSCs derived from mouse, pig, guinea pigs using episomal or retroviral reprogramming methods, and various mouse ESC lines that have been tested for successful germline transmission. These cells are ideal for all iPS cell culture applications such as disease modeling, differentiation into cellular lineages of choice and drug toxicity/efficacy screening.

    • iPSC Differentiated Cells

      We offer fully characterized iPSC-differentiated progenitors and differentiated cell lines derived from integration-free iPSCs using feeder-free culture protocols: Neural stem cells (NSCs), Neurons (Dopaminergic and Cortical neurons), Astrocytes, PBMCs and Cardiomyocytes.

    • Gene Edited iPSC Lines

      ASC’s gene edited iPS Cell Line catalog includes isogenic panels of gene edited lines from control iPSC line (ASE-9109) that are fully characterized and are ideal for disease modeling: (1) neuronal disease-specific isogenic knockout line; (2) lineage-specific reporter gene knock-in lines; (3) safe harbor locus reporter gene knock-in iPS lines.

    • iPSC Generation Kit

      Human EZ- iPSC Generation kits - Reprogram your somatic cells to iPSC with the Retroviral or the Episomal kit.

    • Stem Cell Culture : MEF cells, FBS, Medium

      Use Applied StemCell's stem cell validated mouse embryonic fibroblasts (MEF) cells, FBS, serum/ feeder-free (SFFM) medium, and supplements, for optimal and robust growth of your stem cell (iPSCs and ESCs) lines. 

    • Stem Cell Characterization

      Stem Cell Marker kits. Characterization of embryonic stem cells and induced pluripotent stem cells by pluripotency protein or RNA marker kits and stem cell gene arrays.

  • CRISPR/Cas9 Genome Editing

    • Cell Line Model Generation

      The CRISPRCLEAR™ kits for cell line model generation are a do-it-yourself kits for affordable and efficient cell line model generation. A variety of kits are available for gene knockout, point mutation and knock-in models in human and mouse cell lines.

    • Animal Model Generation

      In vivo-grade, injection-ready CRISPR kits for the generation of transgenic mice and rats. 

       

  • TARGATT™ Genome Editing

    • TARGATT™ Mouse Model Generation

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      ASC’s proprietary TARGATT™ transgenic mouse model generation products offer an affordable do-it-yourself option to generate site-specific transgenic mice very efficiently and in as little as 3 months, in your own animal facility. You can purchase all required components to generate your transgenic "knock-in" mice right here from our ASC website: TARGATT™ mice, TARGATT™ plasmids, transgenic kits for site-specific knock-in of your transgene, and genotyping kits. The TARGATT™ products are manufactured in our ISO:9001 quality management system certified facility.

      Transgenic core facilities currently using the TARGATT™ system, including UCSF, NIH, MaxPlank, Harvard, Colombia and many more!

    • TARGATT™ Knockin Master Cell Line

      Rapid generation of site-directed knock-in cell lines.

      Applied StemCell, Inc., offers a large selection of gene-targeting products including TARGATT™ Site-specific Knock-in Cell Line. The cell line kit includes a master cell line with a TARGATT™ docking site (attP) at a transcriptionally active locus (the Hipp11 locus on chromosome 11; H11), and vectors for TARGATT integrase and DNA integration. With this method, large DNA fragments can be inserted at the H11 locus at high efficiency with robust transgene expression.

  • Genetically Modified Cell Lines / Isogenic Cell Lines

    • Cas9 Expressing Cell Lines

      Cell Lines expressing the CRISPR Cas9 nuclease enable you to carry out CRISPR genome editing applications including cell based assays and drug discovery.  

       

  • Xenograft Model Research Tools

    • Substrate-free Autobioluminescent Cell Lines and Vectors

      Eliminate time consuming substrate injection with Luciferase. We can quickly modify your cell lines to express an autobioluminescent reporter and return them to your laboratory for in vivo imaging. 4 major cell lines (HCT116, HEK293, MCF7, T47D) engineered to be autonomously bioluminescent. Custom Cell Line Developement: we can also quickly modify your cell lines to express an autobioluminescent reporter and return them to your laboratory for in vivo imaging as well. 

       

    • MyEZ Gel™ CDX/PDX Models Injection Media

      MyEZGel CDX/PDX is a protein based solubilized base membrane synthesized from commonly used amino acids, and minerals.  The amino acids include Arginine, Aspartate, Glycine, Isoleucine, Leucine, Lysine, Phenylalanine, Proline, Serine, and Valine. MyEZGel CDX/PDX is compatible with various adhesion proteins and cell growth factors, including but not limited to laminin, collagens, VE-growth factor, fibroblast growth factor, and other growth factors. MyEZGel CDX/PDX is a powerful reagent to package and deliver cancer cells, primary cancer cells, or stem cells in vivo for xenograft animal models.

  • Sample Prep

    • Human Immortalized Fibroblasts

      Human Immortalized Fibroblasts (SV40).

    • PCR Mycoplasma Detection Kit

      Routine monitoring of mycoplasma is an essential and neccessary quality control procedure for all cell culture labs. ASC's PCR Mycoplasma Detection Kit is a very affordable, sensitive and straightforward kit for monitoring and ensuring your cell lines are mycoplasma-free.

    • Immortalization Kit