Human induced pluripotent stem cells (iPSCs) can be derived from somatic cells through a reprogramming process driven by ectopic expression of a defined set of reprogramming factors: Oct4, Sox2, Klf4 and c-Myc. These hiPSCs share the properties of self-renewal and pluripotency with human ES cells, and can therefore be used as a renewable source for all differentiated cell types of the body. Human iPSCs can be generated from patients of virtually any genetic background.
Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. The VSV-G pseudotyped retrovirus has a wide range of targets including both mammalian and non-mammalian cells, and are usually silenced in ES cells. The retrovirus is commonly used in generating iPSCs because of its high reprogramming efficiency. The Human EZ-iPSC Generation Retrovirus Kit offers such opportunity to generate iPSCs from various tissues and cell types with an increased reprogramming efficiency of 0.05-0.5%. The success of iPSC generation and subsequent differentiation relies on the efficient and rapid silencing of exogenous genes. The exogenous genes delivered by retroviruses can be silenced faster than those from lentiviral-based vectors. This makes the retroviral kit a powerful and versatile tool for generating iPSCs from various tissues or cell types.
Kit contains sufficient reagents for 4 reprogramming experiments:
- GFP VSV-G retrovirus
- VSV-G retroviral cocktail (contains: human Oct4 retrovirus, human Sox2 retrovirus, human Klf4 retrovirus, and human c-myc retrovirus)
- Polybrene, 10 mg/mL
- CF1 MEF, irradiated
Progress of reprogramming human fibroblasts: