• iPSC Oligodendrocytes
    • Drug Discovery
    • CNS disease modeling
    iPSC Oligodendrocytes

Oligodendrocytes Differentiation Service

Oligodendrocytes Differentiation Service from iPSCs:

Two Milestones/Project: Milestone 1 (item 1-4): OPC-OLIG2+ differentiation.

Milestone 2 (item 5-7): OPC-O4+ differentiation.

Standard Deliverables:

  • 5-6 million OPC-OLIG2+ cells, cryopreserved, 1 million cells/vial. 1 vial will be recovered for markers staining; 3-4 vials will be recovered for O4+ cells differentiation. 1 frozen vial can be delivered.
  • 2-3 million mixed population cells containing OPC-O4 cells, frozen or live cultures.
  • Reports: OPC-OLIG2+: Pax6 and OLIG2 staining; freeze-thaw recovery test, mycoplasma; OPC-O4+: O4 staining, and freeze-thaw testing result.
Products and Services
Technical Details

Oligodendrocytes Differentiation Service from iPSCs:

Oligodendrocytes Differentiation Service from iPSCs

 Figure. Immunocytochemistry for oligodendrocyte expression marker, GalC (red) and nuclear marker (DAPI).

Two Milestones/Project: Milestone 1 (item 1-4): OPC-OLIG2+ differentiation.

Milestone 2 (item 5-7): OPC-O4+ differentiation.

Standard Deliverables:

  • 5-6 million OPC-OLIG2+ cells, cryopreserved, 1 million cells/vial. 1 vial will be recovered for markers staining; 3-4 vials will be recovered for O4+ cells differentiation. 1 frozen vial can be delivered.
  • 2-3 million mixed population cells containing OPC-O4 cells, frozen or live cultures.
  • Reports: OPC-OLIG2+: Pax6 and OLIG2 staining; freeze-thaw recovery test, mycoplasma; OPC-O4+: O4 staining, and freeze-thaw testing result.
Case Studies

Oligodendrocyte Progenitor Cell (OPCs) Differentiation Service

Project Description:

  • iPSC Differentiation to Precursor Oligodendrocyte Progenitor Cells (OPCs) and Mature OPCs

Turnaround Time: 4-5 months

Timeline (May Vary):

  1. iPSC Recovery & Expansion
  2. Differentiation to Precursor OPCs
  3. Further Differentiation to Mature OPCs
  4. Antibody Staining (Flow Cytometry is Available if Inquired)

QC Data: Antibody Staining

Figure 1: Antibody Staining of the Precursor Oligodendrocyte Progenitor Cells (OPCs). The precursor OPCs, differentiated from Applied StemCell’s control iPSC line, ASE-9211 were stained with the precursor OPC marker O4. Image: O4 (Green)

Figure 2: Antibody Staining of the Mature Oligodendrocyte Progenitor Cells (OPCs). The mature OPCs were stained with the OPC marker MBP. Top Image: MBP (Red); Bottom Image: MBP (Red) and DAPI (Blue)

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