Oligodendrocytes Differentiation Service
Oligodendrocytes Differentiation Service from iPSCs:
Two Milestones/Project: Milestone 1 (item 1-4): OPC-OLIG2+ differentiation.
Milestone 2 (item 5-7): OPC-O4+ differentiation.
Standard Deliverables:
- 5-6 million OPC-OLIG2+ cells, cryopreserved, 1 million cells/vial. 1 vial will be recovered for markers staining; 3-4 vials will be recovered for O4+ cells differentiation. 1 frozen vial can be delivered.
- 2-3 million mixed population cells containing OPC-O4 cells, frozen or live cultures.
- Reports: OPC-OLIG2+: Pax6 and OLIG2 staining; freeze-thaw recovery test, mycoplasma; OPC-O4+: O4 staining, and freeze-thaw testing result.
Oligodendrocytes Differentiation Service from iPSCs:
Figure. Immunocytochemistry for oligodendrocyte expression marker, GalC (red) and nuclear marker (DAPI).
Two Milestones/Project: Milestone 1 (item 1-4): OPC-OLIG2+ differentiation.
Milestone 2 (item 5-7): OPC-O4+ differentiation.
Standard Deliverables:
- 5-6 million OPC-OLIG2+ cells, cryopreserved, 1 million cells/vial. 1 vial will be recovered for markers staining; 3-4 vials will be recovered for O4+ cells differentiation. 1 frozen vial can be delivered.
- 2-3 million mixed population cells containing OPC-O4 cells, frozen or live cultures.
- Reports: OPC-OLIG2+: Pax6 and OLIG2 staining; freeze-thaw recovery test, mycoplasma; OPC-O4+: O4 staining, and freeze-thaw testing result.
Oligodendrocyte Progenitor Cell (OPCs) Differentiation Service
Project Description:
- iPSC Differentiation to Precursor Oligodendrocyte Progenitor Cells (OPCs) and Mature OPCs
Turnaround Time: 4-5 months
Timeline (May Vary):
- iPSC Recovery & Expansion
- Differentiation to Precursor OPCs
- Further Differentiation to Mature OPCs
- Antibody Staining (Flow Cytometry is Available if Inquired)
QC Data: Antibody Staining
Figure 1: Antibody Staining of the Precursor Oligodendrocyte Progenitor Cells (OPCs). The precursor OPCs, differentiated from Applied StemCell’s control iPSC line, ASE-9211 were stained with the precursor OPC marker O4. Image: O4 (Green)
Figure 2: Antibody Staining of the Mature Oligodendrocyte Progenitor Cells (OPCs). The mature OPCs were stained with the OPC marker MBP. Top Image: MBP (Red); Bottom Image: MBP (Red) and DAPI (Blue)
