PBMC and HSC CD34+ Humanized Mice
- On-demand Production
- Ideal for In Vivo Efficacy, Safety & Toxicity Studies

Humanized PBMC and CD34+ Mouse Models

Are you looking to further your preclinical studies and begin your in vivo efficacy, safety, and toxicity experiments? Applied StemCell now supplies research-ready humanized PBMC and CD34+ reconstruction mouse models ideal for infectious disease, gene therapy, hematopoiesis, drug target, and immunogenicity studies. Our humanized models are generated using our M-NSG immunodeficient mice through the injection of healthy human donor peripheral blood mononuclear cells (PBMCs) or CD34+ hematopoietic stem cells (HSCs). After a few weeks of reconstruction following the injection of PBMCs or CD34+ HSCs, the mice develop a human-like immune system and are ready to be shipped. Place your order today and claim your free t-shirt!

Affordable
Created in the U.S.A.
Worldwide shipping
In vivo efficacy, safety, and toxicity testing services with PBMC or CD34 humanized mice are available – Inquire or visit our in vivo assay page

Customizable Services! If you would like our team to produce a PBMC or CD34 humanized mouse model with your PBMCs or CD34+ HSCs, we can inject your cells into our NSG mice to produce the model you require for your research. If you need PBMCs, CD34+ HSCs, or immunodeficient mice for your own lab, we also provide tissue collection/cell isolation services, off-the-shelf cell products, and M-NSG mice. Contact us today to learn more.

Human Immune System Reconstruction Mouse Model Generation

CD34+ Reconstruction	PBMC Reconstruction
1. 4-weeks-old M-NSG female mice are irradiated with 1Gy 12h	1. Ficoll density gradient centrifugation is used to purify human peripheral blood mononuclear cells (PBMCs) from cord blood
2. 1E5 human CD34⁺ hematopoietic stem cells derived from umbilical cord blood are injected into the mice	2. The 5E6 Human PBMCs are injected into the M-NSG mice
3. 16 weeks after the transplantation of human CD34+ HSC (hematopoietic stem cells) into the M-NSG mice, the human HSCs develop a relatively well-developed human-derived immune system	3. After 3 weeks of reconstruction, a relatively well-developed human-derived immune system develops