Newsletter
Humanized PBMC and HSC CD34+ Mouse Models
Human peripheral blood mononuclear cell-engrafted M-NSG mice are perfect models for short-term experiments requiring mature T cells, including immuno-oncology, and GVHD research.
Are you looking to further your preclinical studies and begin your in vivo efficacy, safety, and toxicity experiments? Applied StemCell now supplies research-ready humanized PBMC and CD34+ reconstruction mouse models ideal for infectious disease, gene therapy, hematopoiesis, drug target, and immunogenicity studies. Our humanized models are generated using our M-NSG mice through the injection of healthy human donor peripheral blood mononuclear cells (PBMCs) or CD34+ hematopoietic stem cells (HSCs). After a few weeks of reconstruction following the injection of PBMCs or CD34+ HSCs, the mice develop a human-like immune system and are ready to be shipped.
ASC offers ready-to-use Hu-PBMC and Hu-CD34+ Models that can be shipped directly from our US, AAALAC accredited facility.
- Affordable
- Created in the U.S.A.
- In vivo efficacy, safety, and toxicity testing services with PBMC or CD34 humanized mice are available – Inquire or visit our in vivo assay page
Products and Services
Technical Details
Human Immune System Reconstruction Mouse Model Generation
PBMC vs. CD34+ Models
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PBMC Reconstruction |
CD34+ Reconstruction |
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1. Ficoll density gradient centrifugation is used to purify human peripheral blood mononuclear cells (PBMCs) from cord blood |
1. 4-weeks-old M-NSG female mice are irradiated with 1Gy 12h |
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2. The 5E6 Human PBMCs are injected into the M-NSG mice |
2. 1E5 human CD34+ hematopoietic stem cells derived from umbilical cord blood are injected into the mice |
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3. After 3 weeks of reconstruction, a relatively well-developed human-derived immune system develops |
3. 16 weeks after the transplantation of human CD34+ HSC (hematopoietic stem cells) into the M-NSG mice, the human HSCs develop a relatively well-developed human-derived immune system |
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Hu-PBMC Reconstruction Model |
Hu-CD34+ (Hu-HSC) Reconstruction Model |
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Application Notes
Field Of Application
PBMC Humanized Mouse
- Immuno-oncology study
- GvHD study
- Hematological disease study
- Infectious disease study
- Gene therapy
- Study on drug targets without cross reactions
- Immunogenicity assay
CD34 HSC Humanized Mice
- Immuno-oncology study
- Hematological disease study
- Infectious disease study
- Gene therapy
- Study on drug targets without cross reactions
- Immunogenicity assay
Support Materials
Webinar
Webinar - Humanized M-NSG PBMC and CD34+ Mouse Models (2023)
FAQs
After the injection, how long will it take to deliver domestically in the US?
Where can I order and how can I know the schedule?
How much is the shipping fees, if I order 20 female mice today?
What is the strain you inject the PBMC or CD34+?
Do you have health certificate, example of CDA?
What is the timeline of PBMC injection vs. shipping?
What is the optimal age range for the M-NSG mice to be injected with PBMCs?
How many PBMCs are injected into the M-NSG mice?
Can I request a specific lot of cells or donors to be used for my order?
Is there a minimum number of HuPBMC-NSG mice and CD34+ mice for my order?
When should the HuPBMC-NSG mice be placed on study?
Can I try mice for my evaluation?