• PBMC and HSC CD34+ Humanized Mice
    • On-demand Production
    • Ideal for In Vivo Efficacy, Safety & Toxicity Studies
    PBMC and HSC CD34+ Humanized Mice

Humanized PBMC and CD34+ Mouse Models

Are you looking to further your preclinical studies and begin your in vivo efficacy, safety, and toxicity experiments? Applied StemCell now supplies research-ready humanized PBMC and CD34+ reconstruction mouse models ideal for infectious disease, gene therapy, hematopoiesis, drug target, and immunogenicity studies. Our humanized models are generated using our M-NSG immunodeficient mice through the injection of healthy human donor peripheral blood mononuclear cells (PBMCs) or CD34+ hematopoietic stem cells (HSCs). After a few weeks of reconstruction following the injection of PBMCs or CD34+ HSCs, the mice develop a human-like immune system and are ready to be shipped. Place your order today and claim your free t-shirt!

  • Affordable
  • Created in the U.S.A.
  • Worldwide shipping
  • In vivo efficacy, safety, and toxicity testing services with PBMC or CD34 humanized mice are available – Inquire or visit our in vivo assay page

Customizable Services! If you would like our team to produce a PBMC or CD34 humanized mouse model with your PBMCs or CD34+ HSCs, we can inject your cells into our NSG mice to produce the model you require for your research. If you need PBMCs, CD34+ HSCs, or immunodeficient mice for your own lab, we also provide tissue collection/cell isolation services, off-the-shelf cell products, and M-NSG mice.  Contact us today to learn more.

Human Immune System Reconstruction Mouse Model Generation

 

CD34+ Reconstruction

PBMC Reconstruction

1. 4-weeks-old M-NSG female mice are irradiated with 1Gy 12h

1. Ficoll density gradient centrifugation is used to purify human peripheral blood mononuclear cells (PBMCs) from cord blood

2. 1E5 human CD34+ hematopoietic stem cells derived from umbilical cord blood are injected into the mice

2. The 5E6 Human PBMCs are injected into the M-NSG mice

3. 16 weeks after the transplantation of human CD34+ HSC (hematopoietic stem cells) into the M-NSG mice, the human HSCs develop a relatively well-developed human-derived immune system

3. After 3 weeks of reconstruction, a relatively well-developed human-derived immune system develops

PBMC vs. CD34+ Models

Hu-PBMC Reconstruction Model

Hu-CD34+ (Hu-HSC) Reconstruction Model

  • Easy to establish
  • Efficient T cell engraftment
  • Recall response of human cell donor
  • Poor myeloid cell engraftment
  • Lethal xenogeneic GvHD

 

  • Development of multiple hematopoietic lineages
  • Primary immune response
  • Human T cell restricted to murine MHC

 

Learn More & Place Your Order

PBMC and Primary Cell Product Ordering

Ordering/Request Information

  • Request Information Form  
  • Call 1-866-497-4180
  • Email: info@appliedstemcell.com
  • Contact Us
  • Self-Checkout

Ordering/ Request Information

Shipping & Delivery

  • Worldwide Shipping 

Shipping & Delivery

PBMC & CD34+ Cell Isolation

  • Collection
    • HIPPA & IRB Adherence
    • On-demand, customized sample collection
  • Processing
    • Optimized protocols

Custom Procurement

Custom In Vivo Assays

  • Efficacy
  • Persistence & Exhaustion
  • Safety & Toxicity

Custom In Vivo Assays

 

 

 

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