• FFPE Block Service
    • Custom Protocols & Fast turnaround time
    • Worldwide Shipping
    FFPE Block Service

Custom FFPE Service

Looking for a fast and affordable custom FFPE block service? Applied StemCell can generate the FFPE block you need with your cell line. Our scientists can produce any number of FFPE blocks you need, and they can even make duplicate blocks of the same cell line if needed. If you have specific cell fixation requirements (e.g., cell fixant temperature, fixation time, etc.), simply let our team know and we will work with you to obtain your desired final product. Contact us today to learn more.  

Service Overview

Once we receive at least 1 X 108 of your cells, our scientist can move forward and prepare your FFPE block. If needed, we can culture the cells for you. Our scientists then follow our optimized protocols to fix the cells using buffered formalin (24 hours, typically) and embed your cells into a paraffin block. If requested (Optional), we can prepare FFPE slides or scrolls from the freshly made block we prepared for you. 

Advantages

  • Turnaround time: 7 working days
  • We can culture your cell line
  • Optional Available Services
    • Custom FFPE Processing of Your Cell Lines
      • Slides: Single or Multi-Spot Capabilities
      • Scrolls
    • H&E Staining of Slides
    • Additional QC Testing

Standard Price *price may vary based on the starting materials

  • Cell Pellet Manufacturing From Culture: from a standard cell line (HEK293, RKO) = $1,450.00 USD/block
  • Sectioning:
    • $12.00 USD/section for the 1st and 2nd section
    • 3 sections or more: $8.00 USD/section

FAQs
What starting material do I need to provide?
What is the turnaround time for a Custom FFPE block?
How many cells do you need to prepare a FFPE block?
How do you perform Quality Control (QC) on the FFPE Block?
How do you fix the cells before preparing a FFPE Block?
Products and Services
Technical Details

How to obtain a Custom FFPE Block quote:

 1) Choose a Cell Lines: 

  1. Your own cell line
  2. Our TARGATT™ Line
  3. Lenti OX Lines Offered 

Custom FFPE Block Service

 Optional FFPE Services:

  • Slides:  Custom FFPE slides are the perfect solution to your IHC and ISH application needs.  We offer both single-spotting and custom multi-spotting services. (see images below)
  • Scrolls: Custom FFPE scrolls can be used as reference materials for in vitro diagnostics development or other applications.

FFPE Slide Options:  Single or Multi-Spot Capability available

 Custom FFPE Slides

Typical FFPE preparation protocol we recommend for custom FFPE projects:

  •  Fixation: 10% formaldehyde/1xPBS for 24 hours at 24-26°C
  • Section thickness: 5 µm
  • Cell Density:  >75%
  • Slide Type: SuperFrosttm Plus
Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!

Google