CF-1 MEF Feeder Cells
Derived from CF-1 mouse embryos and used as feeder layers to support the growth of undifferentiated mouse or human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) .
MEF cells serve as feeder cells to support the growth of undifferentiated mouse or human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). MEF cells are isolated from mouse embryos and should be used at an early passage. Before use as feeder cells, MEF cells must be mitotically inactivated by γ-irradiation or mitomycin-C treatment.
CF-1 MEF feeder cells are derived from 13.5 day old mouse embryos and are most commonly used for ESC and iPSC culture.
All MEF cells are manufactured in the US and have an average viability of 95% after thawing. We offer both untreated cells for further expansion and treated cells that can be directly used as a feeder layer.
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CF-1 MEF Feeder Cells
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For more references, visit our reference page.
What is the maximum number of passages for healthy primary MEFs culture? (ASF-1201)
According to Qiu LQ, Lai WS, Stumpo DJ, Blackshear PJ. Mouse Embryonic Fibroblast Cell Culture and Stimulation. Bio-protocol. 2016 Jul;6(13). DOI: 10.21769/bioprotoc.1859. PMID: 28573158; PMCID: PMC5448408, it states, “The maximum number of passages for healthy primary MEFs is approximately 4, as cell growth is slowed beyond passages 4–5. We normally work with MEFs at passages 2–4 to get optimal cellular responses to stimuli.”
After thawing MEFs, cells become p3 and are only recommended to be expanded to p4. Thus, only 1-2 passages can be done before mitomycin C treatment.