• High Quality MEFs
    • Untreated or Irradiated CF1
    • Maintains robust pluripotent stem cells
    High Quality MEFs

CF-1 MEF Feeder Cells

Derived from CF-1 mouse embryos and used as feeder layers to support the growth of undifferentiated mouse or human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) .

MEF cells serve as feeder cells to support the growth of undifferentiated mouse or human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). MEF cells are isolated from mouse embryos and should be used at an early passage. Before use as feeder cells, MEF cells must be mitotically inactivated by γ-irradiation or mitomycin-C treatment.

CF-1 MEF feeder cells are derived from 13.5 day old mouse embryos and are most commonly used for ESC and iPSC culture.

All MEF cells are manufactured in the US and have an average viability of 95% after thawing. We offer both untreated cells for further expansion and treated cells that can be directly used as a feeder layer.

Also be sure to see our DR4 MEF Feeder Cells, Neo-resistant MEF Feeder Cells, and SNL 76/7 (STO Cell Line) MEF Feeder Cells.



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CF-1 MEF Feeder Cells

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  • Kiamehr, M., Viiri, L. E., Vihervaara, T., Koistinen, K. M., Hilvo, M., Ekroos, K., ... & Aalto-Setälä, K. (2017). Lipidomic profiling of patient-specific induced pluripotent stem cell-derived hepatocyte-like cells. Disease Models & Mechanisms, dmm-030841.
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For more references, visit our reference page.

What is the maximum number of passages for healthy primary MEFs culture? (ASF-1201)