Stem Cell Research and Development (R&D) Studies
Applied StemCell (ASC) offers end-to-end induced pluripotent stem cell (iPSC)-based services from reprogramming to differentiation. iPSCs are a powerful tool that can be incorporated into your basic research, drug discovery, drug screening, preclinical cell regeneration projects, and more. ASC first established its iPSC platform 12 years ago and has continuously worked to optimize its protocols and procedures. ASC's comprehensive iPSC platform offers you affordable and effective solutions for your iPSC-related projects so you can focus on research.
We can generate your iPSCs from human (e.g., PBMCs, fibroblasts, etc.) or non-human samples using highly optimized feeder-free protocols. Furthermore, ASC's scientists are seasoned genome editing and differentiation experts that can work with you to generate the iPSC disease in vitro models that fit your project requirements. Unlike other iPSC service providing companies, ASC's iPSC platform includes its proprietary TARGATT™ gene-editing technology which allows you to benefit from fast and site-specific, stable integration of large DNA fragments at transcriptionally active safe harbor loci (H11 or ASC2). During your free consultation, our expert scientists can help you select the appropriate services and technologies and design the most efficient project outline so you save time, money, and effort!
iPSC Generation Services |
iPSC Genome Editing (CRISPR) |
TARGATT™ Genome Editing |
iPSC Differentiation Service |
In addition, ASC offers extended preclinical research and development services, including drug screening of your therapeutic candidates in iPSC-derived cell lines, as well as characterization and assay development for cell regenerative and cell-based therapy projects.
iPSC Characterization Service |
Drug Toxicity and Efficacy Testing |
Cell-Based Assays |
Custom In Vivo Assay Services |
ASC Advantages
- Free Consultation
- Affordable Pricing
- Detailed Project Proposals designed by our expert scientists
- Personalized experimental/project outlines
- Project schedule
- Comprehensive Final Reports
- Survey Notes
- Raw Data
- Calculated/Integrated Results
- Raw and Integrated Figures
- Fast timelines
Contact us today to schedule a free consultation!
Cell-Based Assays
ASC offers a diverse set of iPSC-derived cell-based assays that can be used to screen your early-stage drug candidates:
|
Screening |
Types of Assays |
Estimated Timeline |
|
Cytotoxicity & Cell Viability Assays |
MTT/ MTS cell proliferation assay LDH, Necrosis and Apoptosis assays Luciferase (bioluminescence) expression cAMP level measurement |
4-6 weeks |
|
Mitochondrial Toxicity Testing |
Enzyme activity Volume fraction detection |
2-4 weeks |
|
Functional Assays |
Calcium influx/ imaging Electrophysiology: Multielectrode array (MEA) analysis and Patch clamp recording |
8-12 weeks |
|
Quantitative Gene Expression |
qPCR RNA-seq using NGS (next generation sequencing) |
2-8 weeks |
|
Morphology |
Neurite growth assay Biomarker screening |
2-4 weeks |
|
Custom Assays |
iPSC generation; characterization; gene editing; differentiation Custom assay development |
Based on project requirements |
Looking for something else? Please contact us with your project requirements.
