• For your clinical trials
    • Pre-IND filing consultation
    • Tissue engineering and regenerative medicine
      For your clinical trials

    Stem Cell Research and Development (R&D) Studies

    Applied StemCell (ASC) offers end-to-end induced pluripotent stem cell (iPSC)-based services from reprogramming to differentiation. iPSCs are a powerful tool that can be incorporated into your basic research, drug discovery, drug screening, preclinical cell regeneration projects, and more. ASC first established its iPSC platform 12 years ago and has continuously worked to optimize its protocols and procedures. ASC's comprehensive iPSC platform offers you affordable and effective solutions for your iPSC-related projects so you can focus on research.

    We can generate your iPSCs from human (e.g., PBMCs, fibroblasts, etc.) or non-human samples using highly optimized feeder-free protocols. Furthermore, ASC's scientists are seasoned genome editing and differentiation experts that can work with you to generate the iPSC disease in vitro models that fit your project requirements. Unlike other iPSC service providing companies, ASC's iPSC platform includes its proprietary TARGATT™ gene-editing technology which allows you to benefit from fast and site-specific, stable integration of large DNA fragments at transcriptionally active safe harbor loci (H11 or ASC2). During your free consultation, our expert scientists can help you select the appropriate services and technologies and design the most efficient project outline so you save time, money, and effort!

    iPSC Generation Services

    iPSC Genome Editing (CRISPR)

    TARGATT™ Genome Editing

    iPSC Differentiation Service

    In addition, ASC offers extended preclinical research and development services, including drug screening of your therapeutic candidates in iPSC-derived cell lines, as well as characterization and assay development for cell regenerative and cell-based therapy projects.

    iPSC Characterization Service

    Drug Toxicity and Efficacy Testing

    Cell-Based Assays

    Custom In Vivo Assay Services

    ASC Advantages

    • Free Consultation
    • Affordable Pricing
    • Detailed Project Proposals designed by our expert scientists 
      • Personalized experimental/project outlines
      • Project schedule
    • Comprehensive Final Reports
      • Survey Notes
      • Raw Data 
      • Calculated/Integrated Results
      • Raw and Integrated Figures
    • Fast timelines

    Contact us today to schedule a free consultation!

    Cell-Based Assays

    ASC offers a diverse set of iPSC-derived cell-based assays that can be used to screen your early-stage drug candidates:

    Screening

    Types of Assays

    Estimated Timeline

    Cytotoxicity & Cell Viability Assays

    MTT/ MTS cell proliferation assay

    LDH, Necrosis and Apoptosis assays

    Luciferase (bioluminescence) expression

    cAMP level measurement

    4-6 weeks

    Mitochondrial Toxicity Testing

    Enzyme activity

    Volume fraction detection

    2-4 weeks

    Functional Assays

    Calcium influx/ imaging

    Electrophysiology: Multielectrode array (MEA) analysis and Patch clamp recording

    8-12 weeks

    Quantitative Gene Expression

    qPCR

    RNA-seq using NGS (next generation sequencing)

    2-8 weeks

    Morphology

    Neurite growth assay

    Biomarker screening

    2-4 weeks

    Custom Assays

    iPSC generation; characterization; gene editing; differentiation

    Custom assay development

    Based on project requirements

    Looking for something else? Please contact us with your project requirements.

    Have Questions?

    An Applied StemCell technical expert is happy to help, contact us today!

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