Custom In Vivo Assay Services

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As a long-standing leader in genome editing and stem cell technologies, Applied StemCell also offers fully customizable solutions platforms for downstream assays in animal models. We are NIH Office of Laboratory Animal Welfare (OLAW) Assured and have DEA licenses for Schedule I & II-V drugs.

Our state-of-the-art vivarium is equipped with automated cages & devices for behavioral assessments, sample collection and in vivo measurements (ECG, EEG) of the animals.  We can meet the unique needs of our clients by customizing projects piecemeal to fit any requirement/stage of your research pipeline such as:

  • Designing and engineering research animal disease models
  • Adoptive transfers/transplantation
  • In vivo behavioral analyses
  • Functional screening using electrophysiology, EEG & EMG
  • Drug efficacy & toxicity screening

We have an expert team of scientists with several years of experience in various fields in biomedical research to design a comprehensive project plan perfect for your research. Our project portfolio also comes with dedicated project managers to manage scheduling, communication, and completion of your projects.

Below is a selected list of assays available for your in vivo assessments:

1. Disease Model Generation:

  • Genetically modified mouse and rat models
  • Adoptive cell transfer, teratomas
  • Surgically/ drug induced models

2. In Vivo Assays:

  • Behavioral assessments: cognition & locomotor activity
  • Automated in vivo measurements: pharmacokinetics, ECG, EEG, EMG
  • In vivo pharmacokinetics
3. In Vitro/ Postmortem Assays:
  • Electrophysiology: neuronal & cardiac assays; patch-clamp, MEA
  • Tissue collection and end-of-study analyses: western blots, immunohistochemistry, RT-PCR

Don’t see an assay/ measurement? Please contact us to discuss your requirements with one of our technical experts.

Technical Details

Technical Details

1. Cardiac Ion Channel Safety Screening Using Manual Patch Recording

Screening for potential cardiotoxicity of novel drug candidates that modulate key ion channels. Utilizing our expertise in patch-clamp electrophysiology, drugs can be screened against an array of ion channels including recombinant human ether-a-go-go (hERG), Nav1.5, Cav1.2, and human iPSC-derived cardiomyocytes.

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Figure 1. (A) Several ion channels involved in the cardiac action potential are implicated in QT interval prolongation. (B-D) Example recordings showing drug inhibition of human Nav1.5, Cav1.2 and hERG currents. Black traces represent control currents and red traces show currents in the presence of representative inhibitory drugs.

2. Functional Screening Assays to Test Neurological Endpoints Using Patch Clamp Recording

Electrophysiological Recordings in Rodent Brain Slices for disease model validation and drug efficacy/safety. Standard electrophysiological recordings of acutely prepared brain slices are used to measure changes in membrane potential, cell excitability, spike firing and synaptic neurotransmission, including long-term potentiation/ depression (LTP/LTD).

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Figure 2.  Mice were euthanized and the brains were quickly isolated and placed into ice-cold artificial cerebrospinal fluid (ACSF) continuously bubbled with 5% CO2/95% O2. The ACSF is composed of (in mM) NaCl 124.0, KCl 2.5, KH2PO4 1.2, CaCl2 2.4, MgSO4 1.3, NaHCO3 26.0 and glucose 10.0 (pH 7.4). Slices (260 μm thick) containing hippocampus and/or thalamus were prepared using a vibratome (Leica) and incubated at room temperature in continuously oxygenated ACSF for at least 1 h before recordings at room temperature. Slices were continuously perfused with ACSF bubbled with 5% CO2/95% O2 at a flow rate of 1 mL/min from an elevated reservoir. Recordings were made using an Axon 700B patch clamp amplifier, Axonpatch 1D for extracellular recordings, or a 64-channel multi-electrode array (MEA-64, Multi-Channel Systems).

 

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