Induced Pluripotent Stem Cells (iPSCs) are popular because their pluripotency allows them to differentiate into all three germ layers with the potential to differentiate into all cell types within the body, but unlike ESCs, they are not encumbered with the ethical dispute associated with the sourcing of ESCs. The true potential of stem cell technology lies in the directed terminal differentiation of iPSCs into specific somatic cells which function like primary cells but without the sourcing issue and passaging limitations associated with them. The directed differentiation of iPSCs to glial cells (microglia, astrocytes, and oligodendrocytes) and neuronal lineage cells (neural stem cells and neurons) is of special importance for neurobiology and related disorders, considering the dearth of clinically relevant in vitro models available for research, drug screening, and development, as well as the lack of effective therapy to reverse neuronal damage.
Differentiate your iPSCs into microglia, the resident immune cells of the central nervous system (CNS) for an efficient way to generate in vitro models of neurodevelopment, neuroinflammation, and neurological disorders such as Parkinson’s disease and Alzheimer’s disease. iPSC-derived microglial cells recapitulate the phenotypes and functional properties of primary microglial cells without the sourcing problems associated with them.
Advantages of ASC's Comprehensive iPSC Microglia Differentiation Standard & Custom Services:
- Optimized differentiation protocols
- Receive robust, mature microglia with the morphology of primary microglia
- Cells express key microglial specific markers: IBA1, TMEM119, P2RY12, CX3CR1, TREM2; other markers available upon request
- Differentiate from your healthy, disease or engineered iPSCs
- Control “Master” iPSCs and iPSC Generation are available for deriving your control microglial lines
- Fast turnaround time (2 months)
- GMP iPSC Differentiation Services Available >> Learn More
Optional! Add-on our downstream, cell line validation and phenotype assessment assays for a complete and comprehensive cell line package.
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Case Study 1
iPSC-derived Microglia from Control iPSC Line ASE-9211
Figure 1. Recovery of cryopreserved iPSC-derived microglia (iMGLs). Cryopreserved iMGLs differentiated from Applied StemCell’s control iPSC line, ASE-9211 were recovered in microglia culture media on plates pre-coated with a proprietary microglia-specific coating matrix. The cells were fixed the next day and stained with microglial-specific markers, P2RY12+, TMEM119, IBA1, CX3CR1, TREM2 (top row). Bottom row shows the co-localization of the biomarkers with the nuclear counterstain, DAPI.
Will the iPSC-derived microglia provided be precursors or mature cells?
The cells will be provided as cryopreserved, lineage-committed cells microglial-like cells. Each vial of cells will have requested amount of cell based on cell viability after thawing.
What is the purity of the microglia?
The cells will have >90% purity, confirmed by immunocytochemistry for microglial-specific markers, TMEM119 and P2RY12
What are the quality control criteria you would provide?
In addition to immunocytochemistry for microglial markers, the cells will be tested for sterility, mycoplasma and cell viability before shipment.
What is your differentiation protocol based upon?
Our proprietary differentiation protocol has been optimized using a cytokine-based method.
Do you provide media and protocols along with the microglia?
Yes. We provide 100 mL of media and microglia thawing and culture maintenance protocol along with the cells. The media and protocol have been optimized for microglia differentiated using ASC’s proprietary protocols.
Can the differentiated microglia be passaged?
No. The differentiated microglia are similar to primary cells. They cannot be passaged after maturation.
How long can your differentiated microglia be maintained in culture?
The microglia provided can be maintained in culture for 1-2 weeks post-thaw. They cannot be passaged.